Andrew Ward, PhD
Professor
Department of Integrative Structural and Computational Biology
Research Focus
Investigating membrane proteins and viruses using integrative structural biology techniques.
Membrane protein transporters mediate the passage of a wide variety of molecules into and out of the cell. The molecular details underlying the function of most transporters are still unknown. Thus, my group is interested in studying novel membrane proteins using x-ray crystallography, electron microscopy, and other biophysical techniques. Membrane proteins expressed on the surface of enveloped viruses mediate viral entry into the cell by recognizing cell surface receptors and initiating endocytosis or membrane fusion. Recently, broadly neutralizing antibodies targeting these surface spikes have been shown to provide protection against viruses such as HIV and influenza. We are trying to better understand how these antibodies bind to and neutralize viruses. Using single particle electron microscopy and electron tomography we are able to visualize these complexes and whole viruses, providing information about the mechanism of inhibition and aiding in the design of better immunogens for eliciting a broadly neutralizing antibody response in humans.
Select Publications
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Guenaga, J.; Garces, F.; de Val, N.; Stanfield, Robyn L.; Dubrovskaya, V.; Higgins, Brett W.; Carrette, B.; Ward, Andrew B.; Wilson, Ian A.; Wyatt, Richard T. Glycine substitution at helix-to-coil transitions facilitates the structural determination of a stabilized subtype C HIV envelope glycoprotein. Immunity 2017, 46, 792-+.
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Pejchal, Robert; Doores, Katie J.; Walker, Laura M.; Khayat, Reza; Stanfield, Robyn L.; Ramos, Alejandra; Crispin, Max; Katpally, Umesh; Marozsan, Andre; Cupo, Albert; Liu, Yan; Mcbride, Ryan; Ito, Yukishige; Sanders, Rogier W.; Ogohara, Cassandra; Paulson, James C.; Feizi, Ten; Scanlan, Christopher N.; Moore, John P.; Olson, William C.; Ward, Andrew B.; Poignard, Pascal; Schief, William R.; Burton, Dennis R.; Wilson, Ian A.; Wong, Chi-huey H.; Wang, Sheng-kai K.; Maloveste, Sebastien M.; Julien, Jean-philippe P.; Huang, Po-ssu S.; Depetris, Rafael S. A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield.. Science (New York, N.Y.) 2011, 334, 1097-103.
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Bianchi, M.; Turner, H. L.; Nogal, Bartek; Cottrell, Christopher A.; Oyen, D.; Pauthner, Matthias G.; Bastidas, R.; Nedellec, R.; McCoy, L. E.; Wilson, Ian A.; Burton, Dennis R.; Ward, Andrew B.; Hangartner, Lars Electron-microscopy-based epitope mapping defines specificities of polyclonal antibodies elicited during HIV-1 BG505 envelope trimer immunization. Immunity 2018, 49, 288-300.
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Julien, J. P.; Lee, Jeong Hyun; Cupo, A.; Murin, Charles D.; Derking, R.; Hoffenberg, S.; Caulfield, M. J.; King, C. R.; Marozsan, A. J.; Klasse, P. J.; Sanders, R. W.; Moore, J. P.; Wilson, Ian A.; Ward, Andrew B. Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9. Proceedings of the National Academy of Sciences of the United States of America 2013, 110, 213-218.
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Zhang, L.; Irimia, A.; He, L.; Landais, Elise; Rantalainen, K.; Leaman, D. P.; Vollbrecht, T.; Stano, A.; Sands, D. I.; Kim, A. S.; Poignard, Pascal; Burton, Dennis R.; Murrell, B.; Ward, Andrew B.; Zhu, Jiang; Wilson, Ian A.; Zwick, Michael B.; Miiro, G.; Serwanga, J.; Pozniak, A.; McPhee, D.; Manigart, O.; Mwananyanda, L.; Karita, E.; Inwoley, A.; Jaoko, W.; DeHovitz, J.; Bekker, L. G.; Pitisuttithum, P.; Paris, R.; Allen, S. An MPER antibody neutralizes HIV-1 using germline features shared among donors. Nature Communications 2019, 10.
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Dubrovskaya, V.; Guenaga, J.; de Val, N.; Wilson, R.; Feng, Y.; Movsesyan, A.; Hedestam, G. B.; Ward, Andrew B.; Wyatt, Richard T. Targeted N-glycan deletion at the receptor-binding site retains HIV Env NFL trimer integrity and accelerates the elicited antibody response. PLoS Pathogens 2017, 13.
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Impagliazzo, A.; Milder, F.; Kuipers, H.; Wagner, M. V.; Zhu, X.; Hoffman, R. M.; van Meersbergen, R.; Huizingh, J.; Wanningen, P.; Verspuij, J.; de Man, M.; Ding, Z.; Apetri, A.; Kukrer, B.; Sneekes-Vriese, E.; Tomkiewicz, D.; Laursen, N. S.; Lee, Peter S.; Zakrzewska, A.; Dekking, L.; Tolboom, J.; Tettero, L.; van Meerten, S.; Yu, W.; Koudstaal, W.; Goudsmit, J.; Ward, Andrew B.; Meijberg, W.; Wilson, Ian A.; Radosevic, K. A stable trimeric influenza hemagglutinin stem as a broadly protective immunogen. Science 2015, 349, 1301-1306.
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Kong, Leopold; Giang, Erick; Nieusma, Travis; Kadam, Rameshwar U.; Cogburn, Kristin E.; Hua, Yuanzi; Dai, Xiaoping; Stanfield, Robyn L.; Burton, Dennis R.; Ward, Andrew B.; Wilson, Ian A.; Law, Mansun Hepatitis C virus E2 envelope glycoprotein core structure.. Science (New York, N.Y.) 2013, 342, 1090-4.
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Dreyfus, Cyrille; Laursen, Nick S.; Kwaks, Ted; Zuijdgeest, David; Khayat, Reza; Ekiert, Damian C.; Lee, Jeong H.; Metlagel, Zoltan; Bujny, Miriam V.; Jongeneelen, Mandy; Lamrani, Mohammed; Korse, Hans J.; Geelen, Eric; Sahin, Ozcan; Sieuwerts, Martijn; Brakenhoff, Just P.; Vogels, Ronald; Li, Olive T.; Poon, Leo L.; Peiris, Malik; Koudstaal, Wouter; Ward, Andrew B.; Wilson, Ian A.; Goudsmit, Jaap; Friesen, Robert H.; Vlugt, Remko V. Highly conserved protective epitopes on influenza B viruses.. Science (New York, N.Y.) 2012, 337, 1343-8.
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Thornburg, N. J.; Nannemann, D. P.; Blum, D. L.; Belser, J. A.; Tumpey, T. M.; Deshpande, S.; Fritz, G. A.; Sapparapu, G.; Krause, J. C.; Lee, Jeong Hyun; Ward, Andrew B.; Lee, D. E.; Li, S.; Winarski, K. L.; Spiller, B. W.; Meiler, J.; Crowe, J. E. Human antibodies that neutralize respiratory droplet transmissible H5N1 influenza viruses. Journal of Clinical Investigation 2013, 123, 4405-4409.
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Ward, Andrew B.; Sali, A.; Wilson, Ian A. Biochemistry. Integrative structural biology. Science 2013, 339, 913-915.
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Grover, Rajesh K.; Zhu, Xueyong; Nieusma, Travis; Boreo, Isabel; Macleod, Amanda S.; Mark, Adam; Niessen, Sherry; Kim, Helen J.; Kong, Leopold; Assad-garcia, Nacyra; Kwon, Keehwan; Chesi, Marta; Smider, Vaughn V.; Salomon, Daniel R.; Jelinek, Diane F.; Kyle, Robert A.; Pyles, Richard B.; Glass, John I.; Ward, Andrew B.; Wilson, Ian A.; Lerner, Richard A.; Jones, Teresa M.; Boero, Isabel A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.. Science (New York, N.Y.) 2014, 343, 656-661.
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Lee, S. C.; Bennett, B. C.; Hong, W. X.; Fu, Y.; Baker, K. A.; Marcoux, J.; Robinson, C. V.; Ward, Andrew B.; Halpert, J. R.; Stevens, Raymond C.; Stout, Charles D.; Yeager, Mark J.; Zhang, Qinghai Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins. Proceedings of the National Academy of Sciences of the United States of America 2013, 110, E1203-11.
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