Flow Cytometry Core

Astrios (A)

• Model: MoFlo Astrios EQ
• Software: Summit 6.3
• Function: Cell Sorter
• Location: Immunology & Microbial Science, Room R12
• Optics:
  • 3 detectors for a 355nm Ultraviolet laser
  • 4 detectors for a 405nm Violet laser
  • 9 detectors for a 488nm Blue laser
  • 5 detectors for a 561nm Green-Yellow laser
  • 4 detectors for a 640nm Red laser
• Detector layout: view charts here
• Nozzles:
  • 70 µm – Up to 72 million cells per hour
  • 100 µm – Up to 36 million cells per hour
• Sorting:
  • 1 – 6 sort streams
  • 1.5 ml, 5 ml, 15 ml, 50 ml tubes

Multiwell plates (up to 1536-well)

FluoroFinder: click here to view

Astrios (B)

• Model: MoFlo Astrios EQ
• Software: Summit 6.3
• Function: Cell Sorter
• Location: Immunology & Microbial Science, Room R12
• Optics:
  • 3 detectors for a 405nm Violet laser
  • 9 detectors for a 488nm Blue laser
  • 4 detectors for a 561nm Green-Yellow laser
  • 4 detectors for a 640nm Red laser
• Detector Layout: view charts here
• Nozzles:
  • 70 µm – Up to 72 million cells per hour
  • 100 µm – Up to 36 million cells per hour
• Sorting:
  • 1 – 6 sort streams
  • 1.5 ml, 5 ml, 15 ml, 50 ml tubes
• Multiwell plates (up to 1536-well)
• FluoroFinder: click here to view

Aurora A & B

• Model: Aurora
• Software: SpectroFlo
• Function: Spectral Analyzer
• Location: Immunology & Microbial Science, Room R12
• Optics: Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser enabling more efficient spectrum capture in the 365-829 nm range. No filter changes required for any fluorochrome excited by the 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers.
  • Utilizes
    • 16 detectors from 355nm Violet laser
    • 16 detectors from 405nm Violet laser
    • 14 detectors from 488nm Violet laser
    • 10 detectors from 561nm Violet laser
    • 8 detectors from 640nm Violet laser
• Detector Layout: view charts here

Propel Bigfoot

• Model: Bigfoot
• Software: Sasquatch Software (SQS)
• Function: Cell Sorting (Spectral and conventional)
• Location: Immunology & Microbial Science, Room R12
• Optics: Excitation Lasers
  • 349nm 405nm 445nm 488nm 532nm 561nm 594nm 640nm 785nm
• Optics: Detectors
  • 60 parameters including FSC and SSC
• Detector Layout:
  • 349nm – 12 Parameters
  • 405nm – 12 Parameter
  • 488nm/532nm – 7 Parameters
  • 561nm/594 – 12 Parameters
  • 640nm – 4 Parameters
  • 445nm – 4 Parameters
  • 785nm – 3 Parameters
  • 55 Total Fluorescent Parameters
• Nozzles: 50, 70, 100, 120, and 150µm ceramic nozzle tips with adjustable pressure settings
• Sorting: Up to 6-way sorting into tubes; configurable tube holders include 1.5mL, 5mL, 15mL, and 50mL adapters. Multi-way micro-well plate sorting up to 1536 wells. Temperature control (4-37°C) for all media types.
• Brochure: click here to view

NovoCyte A & B

• Model: NovoCyte 3000 with NovoSampler Pro
• Software: BD FACS Diva 6
• Function: Analyzer
• Location: Immunology & Microbial Science, Room R12
• Optics:
  • 6 detectors for a 405nm Violet laser
  • 7 detectors for a 488nm Blue laser
  • 2 detectors for a 640nm Red laser
• Detector Layout: view charts
• Fluoro Finder: click here to view
• Brochure: click here to view

NovoCyte Quanteon A/Agilent/Analyzer

• Model: Quanteon 4025 with NovoSampler Q
• Software: NovoExpress
• Function: Analyzer
• Location: Immunology & Microbial Science, Room R12
• Optics:
  • Excitation Lasers: 488nm, 640nm, 405nm, 561nm
  • Detection Parameters: 25 fluorescence + 2 scatter
• Detector Layout: [view chart here]
• Quanteon: [click here to view]

Sony MA900 (A & B)

• Model: MA900
• Software: MA900 Software
• Function: Cell Sorter
• Location: Immunology & Microbial Science, Room R12
• Optics: Excitation Lasers: 488 nm, 638 nm, 405 nm, 561 nm
• Detection Parameters: 12 fluorescence + 2 scatter
• Detector Layout: view charts here
• Nozzles:
  • 70 μm – Up to 50 million cells per hour
  • 100 μm – Up to 30 million cells per hour
• Sorting:
  • 1-4 Sort Streams
  • 0.5-mL, 1.5-mL, 5-mL and 15-mL tubes
  • 96 multiwell plates, PCR plates
• Brochure: click here to view

Yeti

• Model: ZE5
• Software: Everest
• Function: Analyzer
• Location: Immunology & Microbial Science, Room R12
• Optics:
  • 5 detectors for a 355nm Ultraviolet laser
  • 7 detectors for a 405nm Violet laser
  • 5 detectors for a 488nm Blue laser
  • 7 detectors for a 561nm Green-Yellow laser
  • 4 detectors for a 640nm Red laser
• Detector Layout: view graphs here
• Brochure: click here to view

FACSDiscover S8 Cell Sorter

• Model: FACSDiscover S8 Cell Sorter
• Software: FACSChorus
• Function: Cell Sorting (spectral and imaging)
• Location: Immunology & Microbial Science, Room R12
• Optics: Excitation Lasers
  • 349nm 405nm 488nm 561nm 637nm
• Detectors
  • 78 fluorescence APD detectors
• Detector Layout:
  • Fluorescence:
    • 349 nm – 22 UV detectors, 365-860nm
    • 405 nm – 20 Violet detectors, 410-860nm
    • 488 nm – 16 Blue detectors, 495-860 nm
    • 561m – 12 Yellow-green detectors, 570-860nm
    • 637nm – 8 Red detectors, 645-860nm
  • Imaging 488nm:
    • FL1: LP505: 534/46
    • FL2: LP570: 600/60
    • FL3: LP675: 788/225
• Nozzles:
  • 85µm, 35psi, 57KHz
  • 100µm, 20psi, 35KHz
  • 130µm, 7psi, 15KHz
• Sorting:
  • 85µm Up to 6-way sorting into 1.5 & 5ml tubes 100 & 130 2-way sorting in 1.5 & 5 ml tubes
  • Plate sorting up to 384 well plates
• Brochure: click here to view

• Request Access to Flow Cytometery
  • [Internal Scripps User Access Request]
  • [Infinity User Guide]
  • External User Access: email [email protected]

• [Policies & Guidelines]

• [Analyzer Cleanup]– All users are responsible for properly cleaning and/or shutting down the analyzers when they finish. Click the link for details.

• [Sort Buffer Recipe]– Use this recipe as a starting point for your sorting buffer.

• [Sort Optimization]– Here are recommendations for cell preparation for sorting.

• [Current Protocols in Cytometry] – Scripps Research Network or valid login only

• [Cytometry Protocols] – Purdue University Cytometry Laboratories

• [FluoroFinder] – Use this tool to develop fluorescent panels and source antibodies.

• [Fluorescence Spectra Analyzer] – BioLegend

• [Spectra Viewer] – Chroma Technology Corp

• BD Biosciences
  • [Introduction to Flow Cytometry Web-Based Training] 
  • [Multicolor Flow Cytometry Resources] 
  • [Multicolor Flow Cytometry Tools] 
  • [Multicolor Flow Cytometry Webinars] 
• Life Technologies
  • [Flow Cytometry Resource Center] 
• J. Paul Robinson 
  • [Introduction to Cytometry I] 
  • [Introduction to Cytometry II] 
• Molecular Probes
  • [Flow Cytometry Webinars] 
• FlowJo
  • [Cytometry Data Analysis Tutorials] 
• Michael G. Ormerod
  • [Flow Cytometry – A Basic Introduction] 
• Howard M. Shapiro
  • [Practical Flow Cytometry] 

• [ISAC – International Society for Advancement of Cytometry]

• [Southern California Flow Cytometry Association]

• [Purdue Cytometry Discussion List]

Aurora

• 5 Laser Aurora User Manual

• 5L Fluorochrome Information Summary

• 4L Fluorochrome Information Summary

LSR

• BD FACSDiva v6_LSR_Quick Ref Guide

• BD FACSDiva Software Basic Experiment Guide

• BD FACSDiva_Software_v_8.0_manual

• BD LSR II User Guide

NovoCyte

• NovoCyte_Quick_Start_Guide

• NovoExpress-Software-Guide

• NovoCyte Operator Guide

Sony MA900

• MA900_Operators_Guide

ZE5

• ZE5 BioRad Cell Analyzer and Everest Software User Manual V3.1
• ZE5 Quick Start Guide V3.1