Gabriel Ozorowski, PhD

Institute Investigator

Department of Integrative Structural and Computational Biology

Research Focus

Visualizing antibody responses to viral vaccines or infection using electron microscopy

My research focuses on understanding the atomic level interactions between antibodies induced by vaccination or natural infection, and viral glycoproteins. Cryo-EM methods are used to characterize isolated, recombinant monoclonal antibodies, with a focus on HIV vaccine research. In addition, I use electron microscopy to image and epitope map serum polyclonal antibodies in complex with vaccine immunogens, a technique named EMPEM. Combining high-resolution cryo-EM with EMPEM (cryoEMPEM), we can understand the specific amino acids of the antigen contacted by polyclonal antibodies and also predict possible sequences of the antibodies themselves, accelerating vaccine design and antibody discovery.

Education

Professional Experience

Awards & Professional Activities

Select Publications

Steichen, Jon M.; Phung, Ivy; Salcedo, Eugenia; Ozorowski, Gabriel; Willis, Jordan R.; Baboo, Sabyasachi; Liguori, Alessia; Cottrell, Christopher A.; Torres, Jonathan L.; Madden, Patrick J.; Ma, Krystal M.; Sutton, Henry J.; Lee, Jeong Hyu H.; Kalyuzhniy, Oleksandr; Allen, Joel D.; Rodriguez, Oscar L.; Adachi, Yumiko; Mullen, Tina-Mari M.; Georgeson, Erik; Kubitz, Michael; Burns, Alison; Barman, Shawn; Mopuri, Rohini; Metz, Amanda; Altheide, Tasha K.; Diedrich, Jolene K.; Saha, Swati; Shields, Kaitlyn; Schultze, Steven E.; Smith, Melissa L.; Schiffner, Torben; Burton, Dennis R.; Watson, Corey T.; Bosinger, Steven E.; Crispin, Max; Yates, John R.; Paulson, James C.; Ward, Andrew B.; Sok, Devin; Crotty, Shane; Schief, William R. Vaccine priming of rare HIV broadly neutralizing antibody precursors in nonhuman primates. 2024, 384, eadj8321.
Hahn, William O.; Parks, K Rachae R.; Shen, Mingchao; Ozorowski, Gabriel; Janes, Holly; Ballweber-Fleming, Lamar; Woodward Davis, Amanda S.; Duplessis, Chris; Tomai, Mark; Dey, Antu K.; Sagawa, Zachary K.; De Rosa, Stephen C.; Seese, Aaron; Kallur Siddaramaiah, Latha; Stamatatos, Leonidas; Lee, Wen-Hsi H.; Sewall, Leigh M.; Karlinsey, Dalton; Turner, Hannah L.; Rubin, Vanessa; Furth, Sarah; MacPhee, Kellie; Duff, Michael; Corey, Lawrence; Keefer, Michael C.; Edupuganti, Srilatha; Frank, Ian; Maenza, Janine; Baden, Lindsey R.; Hyrien, Ollivier; Sanders, Rogier W.; Moore, John P.; Ward, Andrew B.; Tomaras, Georgia D.; Montefiori, David C.; Rouphael, Nadine; McElrath, M Julian J. Use of 3M-052-AF with Alum adjuvant in HIV trimer vaccine induces human autologous neutralizing antibodies. 2024, 221.
Antanasijevic, Aleksandar; Bowman, Charles A.; Kirchdoerfer, Robert N.; Cottrell, Christopher A.; Ozorowski, Gabriel; Upadhyay, Amit A.; Cirelli, Kimberly M.; Carnathan, Diane G.; Enemuo, Chiamaka A.; Sewall, Leigh M.; Nogal, Bartek; Zhao, Fangzhu; Groschel, Bettina; Schief, William R.; Sok, Devin; Silvestri, Guido; Crotty, Shane; Bosinger, Steven E.; Ward, Andrew B. From structure to sequence: Antibody discovery using cryoEM. 2022, 8, eabk2039.
Ozorowski, Gabriel; Torres, Jonathan L.; Santos-Martins, Diogo; Forli, Stefano; Ward, Andrew B. A Strain-Specific Inhibitor of Receptor-Bound HIV-1 Targets a Pocket near the Fusion Peptide. 2020, 33, 108428.
Torres, Jonathan L.; Ozorowski, Gabriel; Andreano, Emanuele; Liu, Hejun; Copps, Jeffrey; Piccini, Giulia; Donnici, Lorena; Conti, Matteo; Planchais, Cyril; Planas, Delphine; Manganaro, Noemi; Pantano, Elisa; Paciello, Ida; Pileri, Piero; Bruel, Timothée; Montomoli, Emanuele; Mouquet, Hugo; Schwartz, Olivier; Sala, Claudia; De Francesco, Raffaele; Wilson, Ian A.; Rappuoli, Rino; Ward, Andrew B. Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody. 2022, 119, e2120976119.
Ozorowski, Gabriel; Pallesen, Jesper; de Val, Natalia; Lyumkis, Dmitry; Cottrell, Christopher A.; Torres, Jonathan L.; Copps, Jeffrey; Stanfield, Robyn L.; Cupo, Albert; Pugach, Pavel; Moore, John P.; Wilson, Ian A.; Ward, Andrew B. Open and closed structures reveal allostery and pliability in the HIV-1 envelope spike. 2017, 547, 360-363.