Scripps Research Logo

The Wüthrich Laboratory

Current Research Projects

Current research of the Wüthrich Team includes all aspects of biomolecular NMR spectroscopy in solution and structural interpretation of NMR data. In addition to NMR equipment at 1H frequencies of 600, 700 and 800 MHz, with attachments for micro-coil experiments and 19F-detection, the team runs a molecular biology/biochemistry laboratory for functional and biophysical studies of proteins, and for the preparation of isotope-labeled proteins for NMR studies. The main focus of our research is on the use of NMR within the framework of the Joint Center for Structural Genomics (JCSG www.jcsg.org), the JCSG Center for Innovative Membrane Protein Technologies (JCIMPT-Complexes http://www.jcimpt.scripps.edu/) and the GPCR-Network (http://www.gpcr.scripps.edu/). As part of these protein structure initiatives, we develop and apply NMR methods for efficient serial screening of soluble and membrane protein targets for their suitability for NMR or crystal structure determination, using microcoil NMR equipment combined with microexpression of proteins, and for NMR structure determination of selected proteins and their complexes with small-molecule ligands or other biological macromolecules.  A special focus is on the use of solution NMR techniques for studies of dynamics and function of GPCRs.