Scripps Research Logo

The Wüthrich Laboratory

Current Research Projects

Current research of the Wüthrich Team includes all aspects of biomolecular NMR spectroscopy in solution and structural interpretation of NMR data. In addition to NMR equipment at 1H frequencies of 700 and 800 MHz, with peripheral hardware for micro-coil experiments and high-sensitivity 19F-detection, the team operates a molecular biology/biochemistry laboratory for functional and biophysical studies of proteins, in particular for investigations of protein-protein, protein-nucleic acid and protein-lipid interactions, and for the preparation of isotope-labeled proteins in supramolecular complexes for NMR studies. Present applications of solution NMR techniques include studies of opioid receptors and other GPCRs, where a special focus is on studies of function-related local polymorphisms and the dynamic processes governing transitions between locally different conformations in inactive and activate-like states of GPCRs. Other projects are focused on investigations of intramolecular rate processes in globular proteins in solution, in particular dynamic processes manifested by "ring flips" of phenylalanine and tyrosine.