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The Catz Laboratory


Selected Publications

for complete list of publications please refer to

Jennifer L. Johnson,  Mahalakshmi Ramadass, Jing He, Steve Brown,  William Kiosses,  Jinzhong Zhang, Nikolaos Biris, Evripidis Gavathiotis, Hugh Rosen and Sergio D. Catz. Identification of Nexinhibs, small-molecule inhibitors of neutrophil exocytosis and inflammation. Druggability of the small GTPase Rab27a. 2016, J. Biol. Chem. 2016 Oct 4. pii: jbc.M116.741884[Epub ahead of print]

Angela R M Kurz, Monika Pruenster, Ina Rohwedder, Mahalakshmi Ramadass, Kerstin Schäfer, Ute Harrison, Gabriel Gouveia, Claudia Nussbaum, Roland Immler, Johannes R Wiessner, Andreas Margraf, Dae-Sik Lim, Barbara Walzog, Steffen Dietzel, Markus Moser, Christoph Klein, Dietmar Vestweber, Rainer Haas, Sergio D. Catz and Markus Sperandio. MST1-dependent vesicle trafficking regulates neutrophil transmigration through the basement membrane. Journal of Clinical Investigation, 2016, Oct 4. pii: 87043. doi: 10.1172/JCI87043

Mahalakshmi Ramadass, Jennifer L. Johnson, and Sergio D. Catz. Rab27a regulates GM-CSF-dependent priming of neutrophil exocytosis. Journal of Leukocyte Biology, 2016, Oct 12. pii: jlb.3AB0416-   189RR. [Epub ahead of print]

Mahalakshmi Ramadass and Sergio D. Catz. Molecular Mechanisms Regulating Secretory Organelles and Endosomes in Neutrophils and their Implications for Inflammation. 2016, Immunological Reviews,      273(1):249-65

Laura Rita Rega; Elena Polishchuk; Sandro Montefusco;Gennaro Napolitano; Giulia Tozzi; Jinzhong Zhang; Francesco Bellomo; Anna Taranta; Anna Pastore; Roman Polishchuk; Fiorella Piemonte; Diego        Luis Medina; Sergio D. Catz; Olivier Devuyst; Andrea Ballabio; Francesco Emma. Activation of the transcription factor EB promotes clearance of lysosomal cystine and rescues abnormalities of the  lysosomal compartment in cystinotic cells. 2016, Kidney International, 89(4):862-73.

Jennifer L. Johnson, Jing He, Mahalakshmi Ramadass, Kersi Pestonjamasp, William Kiosses,  Jinzhong Zhang and Sergio D. Catz. Munc13-4 is a Rab11-binding protein that regulates Rab11-positive vesicle  trafficking and docking at the plasma membrane. 2016, Journal of Biological Chemistry, 291(7):3423-38

Jing He, Jennifer L. Johnson, Jlenia Monfregola, Mahalakshmi Ramadass, Kersi Pestonjamasp and Sergio D. Catz. Munc13-4 interacts with syntaxin 7, regulates late endosomal  maturation, endosomal  signaling and TLR9-initiated cellular responses. 2016, Molecular Biology of the Cell. 27(3):572-87.

Gennaro Napolitano, Jennifer L. Johnson, Celine J Rocca, Jlenia Monfregola, Stephanie Cherqui and Sergio D. Catz. Deficiency of cystinosin causes impaired chaperone-mediated autophagy independent of      lysosomal overload. EMBO Molecular Medicine 2015, 7(2):158-74. Catz SD. The role of Rab27a in the regulation of neutrophil function. 2014 Cell Microbiol. 2014, 16(9):1301-10 Jun 25. doi:    10.1111/cmi.12328.

Tomoyuki Sasaki, Jie Qi, Peter E. Bayliss, Jennifer L. Johnson, Sergio D. Catz, Matthew Gill, Kailiang Jia, Daniel J. Klionsky, and Shuji Kishi. Aberrant Autolysosomal Regulation Is Linked to The Induction of    Embryonic Senescence Differentially through Beclin 1 and p53 in Vertebrate Spns1 Deficiency. 2013 Plos Genetics, PLoS Genet. 2014 Jun 26;10(6):e1004409. doi: 10.1371

Johnson JL, Napolitano G, Monfregola J, Rocca CJ, Cherqui S and Catz SD. Upregulation of the Rab27a-dependent trafficking mechanism improves lysosomal transport, alleviates endoplasmic reticulum stress and reduces cystine content in cystinotic cells. Mol Cell Biol. 2013. 33:2950-62

Catz SD. Regulation of vesicular trafficking and leukocyte function by Rab27 GTPases and their effectors. Journal of Leukocyte Biology. 2013 Epub ahead of print February 1, 2013 - doi:10.1189/jlb.1112600

Singh RK, Mizuno K, Wasmeier C, Wavre-Shapton ST, Recchi C, Catz SD, Futter C, Tolmachova T, Hume AN, Seabra MC. Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion. FEBS J. 2012 Nov 29. doi: 10.1111/febs.12081.

Monfregola J, Johnson JL, Meijler MM, Napolitano G, Catz SD. Munc13-4 protein regulates the oxidative response and is essential for phagosomal maturation and bacterial killing in neutrophils. J Biol Chem. 2012. 287(53):44603-44618 PMID: 23115246

Johnson JL, Monfregola J, Napolitano G, Kiosses WB, Catz SD. Vesicular trafficking through cortical actin during exocytosis is regulated by the Rab27a effector JFC1/Slp1 and the RhoA-GTPase-activating protein Gem-interacting protein. Mol. Biol. Cell. 2012. 23(10):1902-16.

Johnson JL, Hong H, Monfregola J, Catz SD.  Increased survival and reduced neutrophil infiltration of the liver in Rab27a- but not Munc13-4-deficient mice in LPS-induced systemic inflammation. Infection & Immunity, 2011; Sep;79(9):3607-18

Johnson JL, Hong H, Monfregola J, Kiosses WB, and Catz SD. MUNC13-4 restricts motility of RAB27A-expressing vesicles to facilitate lipopolysaccharide-induced priming of exocytosis in neutrophils. J. Biol. Chem., 2011 286(7):5647-56

Johnson JL, Brzezinska AA, Tolmachova T, Munafo DB, Ellis BA, Seabra MC, Hong H, Catz SD. Rab27a and Rab27b Regulate Neutrophil Azurophilic Granule Exocytosis and NADPH oxidase Activity by Independent Mechanisms. Traffic, 2010; 11, 533-547

Daniela B. Munafo, Jennifer L. Johnson, Agnieszka A. Brzezinska, Beverly A. Ellis, Malcolm R. Wood and Catz SD. DNase I inhibits a late phase of reactive oxygen species production in neutrophils. J Innate Immunity 2009; 1:527–542

Agnieszka A. Brzezinska, Jennifer L. Johnson, Daniela B. Munafo, Beverly A. Ellis and Catz.S.D Signaling mechanisms for Toll-like receptor-activated neutrophil exocytosis: key roles for IRAK-4 and PI3-kinase but not TRIF. Immunology, 2009; 127(3):386-97  

Agnieszka A. Brzezinska, Jennifer L. Johnson, Daniela B. Munafo, Karine Crozat, Bruce Beutler, William B. Kiosses, Beverly A. Ellis and Catz S.D The Rab27a effectors JFC1/Slp1 and Munc13-4 regulate exocytosis of neutrophil granules. Traffic, 2008; 9: 2151–2164

Catz S.D. Characterization of Rab27a and JFC1 as constituents of the secretory machinery of prostate-specific antigen in prostate carcinoma cells. Methods in Enzymology, 2008; 438: 25-40

Pacquelet S, Johnson JL, Ellis BA, Brzezinska AA, Lane WS, Munafo DB and Catz S.D. (2007) Cross-talk between IRAK-4 and the NADPH oxidase. Biochemical Journal. 403(3):451-61.

Munafó DB, Johnson JL, Ellis BA, Rutschmann S, Beutler B and Catz S.D. (2007) Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytes. Biochemical Journal. 402(2):229-39

J.L. Johnson, B.A. Ellis, D. Noack, M.C. Seabra and Catz, S.D. (2005). The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphatase. Biochemical Journal. 391: 699–710.

J. L. Johnson, S. Pacquelet, W.S. Lane, B. Eam and Catz, S.D. (2005) Akt Regulates the Subcellular Localization of the Rab27a-Binding Protein JFC1 by Phosphorylation. Traffic 6(8):667-81.

Catz, S.D., Johnson, J.L. & Babior, B.M. (2002) The C2A domain of JFC1 binds to 3’-phosphorylated phosphoinositides and directs plasma membrane association in living cells. Proc.Natl.Acad.Sci.U.S.A. 99(18):11652-11657.

Catz, S.D., Babior, B.M. & Johnson, J.L. (2002) JFC1 is transcriptionally activated by nuclear factor kB and up-regulated by tumor necrosis factor a in prostate carcinoma cells. Biochemical Journal, 367:791-9.

Catz, S.D. & Johnson, J.L. (2001) Transcriptional regulation of bcl-2 by nuclear factor kappaB and its significance in prostate cancer. Oncogene, 20, 7342-7351.