The Rosen Laboratory

PUBLICATIONS

1. Sarkisyan G, Cahalan S, Gonzalez-Cabrera P, Leaf N, Rosen H. (2012) Real-time differential labeling of blood, interstitium and lymphatic and single-field analysis of vasculature dynamics in vivo. Am J Physiol Cell Physiol. 2012 Feb 22. [Epub ahead of print] PMID: 22357735

2. Hanson M, Roth C, Jo E, et al. (2012) Crystal Structure of a Lipid G-protein Coupled Receptor. Science 335(6070):851-5. PMID: 22344443

Click here to view the NIH press release

3. Guerrero M, Urbano M, Zhao J, et al. Discovery, design and synthesis of novel potent and selective sphingosine-1-phosphate 4 receptor (S1P(4)-R) agonists. Bioorg Med Chem Lett. 2012 Jan 1;22(1):537-42.

4. Walsh KB, Teijaro JR, Wilker PR, et al. Suppression of cytokine storm with a sphingosine analog provides protection against pathogenic influenza virus. Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):12018-23.

5. Walsh KB, Teijaro JR, Rosen H, Oldstone MB. Quelling the storm: utilization of sphingosine-1-phosphate receptor signaling to ameliorate influenza virus-induced cytokine storm. Immunol Res. 2011 Oct;51(1):15-25.

6. Urbano M., M. Guerrero, J. Zhao, S. Velaparthi, M. T. Schaeffer, S. Brown, H. Rosen and E. Roberts (2011) SAR analysis of innovative selective small molecule antagonists of sphingosine-1-phosphate 4 (S1P) receptor. Bioorg Med Chem Lett. 21(18):5470-4

7. Urbano M, Guerrero M, Velaparthi S, Crisp M, Chase P, Hodder P, Schaeffer MT, Brown S, Rosen H, Roberts E. (2011) Discovery, synthesis and SAR analysis of novel selective small molecule S1P(4)-R agonists based on a (2Z,5Z)-5-((pyrrol-3-yl)methylene)-3-alkyl-2-(alkylimino)thiazolidin-4-one chemotype. Bioorg Med Chem Lett. 2011 Sep 20. [Epub ahead of print] PMID: 21982495

8. Walsh KB, Teijaro JR, Rosen H, Oldstone MB. (2011) Quelling the storm: utilization of sphingosine-1-phosphate receptor signaling to ameliorate influenza virus-induced cytokine storm. Immunol Res. 2011 Oct;51(1):15-25.PMID: 21901448

9. Tsuboi K, Bachovchin DA, Speers AE, Spicer TP, Fernandez-Vega V, Hodder P, Rosen H, Cravatt BF. (2011) Potent and selective inhibitors of glutathione s-transferase omega 1 that impair cancer drug resistance. J Am Chem Soc. 2011 Oct 19;133(41):16605-16. Epub 2011 Sep 27. PMID: 21899313

10. John R. Teijaro, Kevin B. Walsh, Stuart Cahalan, Daniel M. Fremgen, Edward Roberts, Fiona Scott, Esther Martinborough, Robert Peach, Michael B.A. Oldstone, Hugh Rosen. (2011) Endothelial Cells Are Central Orchestrators of Cytokine Amplification during Influenza Virus Infection. Cell 146(6);980-991.

Click here to view the interview with co-authors and Principal Investigators Michael B.A. Oldstone and Hugh Rosen
Click here to read the Leading Edge Preview written by Akiko Iwasaki and Ruslan Medzhitov titled "A New Shield for a Cytokine Storm"

11. Urbano M, Guerrero M, Zhao J, Velaparthi S, Schaeffer MT, Brown S, Rosen H, Roberts E. SAR analysis of innovative selective small molecule antagonists of sphingosine-1-phosphate 4 (S1P(4)) receptor. Bioorg Med Chem Lett. 2011 Sep 15;21(18):5470-4. Epub2011 Jul 13. PubMed PMID: 21783362; PubMed Central PMCID: PMC3159836.

12. Walsh KB, Teijaro JR, Rosen H, Oldstone MB. Quelling the storm: utilization of sphingosine-1-phosphate receptor signaling to ameliorate influenza virus-induced cytokine storm. Immunol Res. 2011 Sep 8. [Epub ahead of print] PubMed PMID:21901448.

13. Tsuboi K, Bachovchin DA, Speers AE, Spicer TP, Fernandez-Vega V, Hodder P,Rosen H, Cravatt BF. Potent and Selective Inhibitors of Glutathione S-transferaseOmega 1 that Impair Cancer Drug Resistance. J Am Chem Soc. 2011 Sep 7. [Epubahead of print]PubMed PMID: 21899313.

14. Lone AM, Bachovchin DA, Westwood DB, Speers AE, Spicer TP, Fernandez-Vega V,Chase P, Hodder PS, Rosen H, Cravatt BF,Saghatelian A. A substrate-free activity-based protein profiling screen for the discovery of selective PREPL inhibitors. J Am Chem Soc. 2011 Aug 3;133(30):11665-74. Epub 2011 Jul 12. PubMed PMID: 21692504; PubMed Central PMCID: PMC3145007.

15. Bachovchin DA, Zuhl AM, Speers AE, Wolfe MR, Weerapana E, Brown SJ, Rosen H, Cravatt BF. Discovery and optimization ofsulfonyl acrylonitriles as selective, covalent inhibitors of protein phosphatase methylesterase-1. J Med Chem. 2011 Jul 28;54(14):5229-36.Epub 2011 Jun 30. PubMed PMID: 21639134; PubMed Central PMCID: PMC3144155.

16. Walsh KB, Teijaro JR, Wilker PR, Jatzek A, Fremgen DM, Das SC, Watanabe T, Hatta M, Shinya K, Suresh M, Kawaoka Y, Rosen H, Oldstone MB. Suppression of cytokine storm with a sphingosine analog provides protection against pathogenic influenza virus. ProcNatl Acad Sci U S A. 2011 Jul 19;108(29):12018-23. Epub 2011 Jun 29. PubMed PMID: 21715659; PubMed Central PMCID: PMC3142000.

17. Guerrero M, Urbano M, Velaparthi S, Zhao J, Schaeffer MT, Brown S, Rosen H, Roberts E. Discovery, design and synthesis of the first reported potent and selective sphingosine-1-phosphate 4 (S1P4) receptor antagonists. Bioorg Med Chem Lett. 2011 Jun 15;21(12):3632-6. Epub 2011 Apr 28. PubMed PMID: 21570287; PubMed Central PMCID: PMC3107912.

18. Cahalan SM, Gonzalez-Cabrera PJ, Sarkisyan G, Nguyen N, Schaeffer MT, Huang L, Yeager A, Clemons B, Scott F, Rosen H. Actions of a picomolar short-acting S1P₁ agonist in S1P₁-eGFP knock-in mice. Nat Chem Biol. 2011 May;7(5):254-6. Epub 2011 Mar 27.PubMed PMID: 21445057.

19. Bachovchin DA, Mohr JT, Speers AE, Wang C, Berlin JM, Spicer TP, Fernandez-Vega V, Chase P, Hodder PS, Schürer SC, Nomura DK, Rosen H, Fu GC, Cravatt BF. Academic cross-fertilization by public screening yields a remarkable class of protein phosphatasemethylesterase-1 inhibitors. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6811-6. Epub 2011 Mar 11. PubMed PMID: 21398589;PubMed Central PMCID: PMC3084096.

20. Chou TF, Brown SJ, Minond D, Nordin BE, Li K, Jones AC, Chase P, Porubsky PR, Stoltz BM, Schoenen FJ, Patricelli MP, Hodder P, Rosen H, Deshaies RJ. Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4834-9. Epub 2011 Mar 7. PubMed PMID: 21383145; PubMed Central PMCID: PMC3064330.

21. Chou TF, Brown SJ, Minond D, Nordin BE, Li K, Jones AC, Chase P, Porubsky PR, Stoltz BM, Schoenen FJ, Patricelli MP, Hodder P, Rosen H,Deshaies RJ. (2011) Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc Natl AcadSci U S A. 2011 Mar 22;108(12):4834-9. Epub 2011 Mar 7. PMID: 21383145

22. Marsolais D, Yagi S, Kago T, Leaf N, Rosen H. (2011) Modulation of chemokines and allergic airway inflammation by selective local S1P1 agonism in lung. Mol Pharmacol. 79(1):61-8. PMID: 20935081

23. Knuckley B, Jones JE, Bachovchin DA, Slack J, Causey CP, Brown SJ, Rosen H, Cravatt BF, Thompson PR. (2010) A fluopol-ABPP HTS assay to identify PAD inhibitors. Chem Commun (Camb). 46(38):7175-7. PMID: 20740228

24. Gianni D, Taulet N, Zhang H, Dermardirossian C, Kister J, Martinez L, Roush WR, Brown SJ, Bokoch GM, Rosen H. (2010) A Novel and Specific NADPH Oxidase-1 (Nox1) Small-Molecule Inhibitor Blocks the Formation of Functional Invadopodia in Human Colon Cancer Cells. ACS Chem Biol. 5(10):981-93. PMID: 20715845

25. Bachovchin DA, Wolfe MR, Masuda K, Brown SJ, Spicer TP, Fernandez-Vega V, Chase P, Hodder PS, Rosen H, Cravatt BF. (2010) Oxime esters as selective, covalent inhibitors of the serine hydrolase retinoblastoma-binding protein 9 (RBBP9). Bioorg Med Chem Lett. 20(7):2254-8. Epub 2010 Feb 6. PMID: 20207142

26. Walsh KB, Marsolais D, Welch MJ, Rosen H, Oldstone MB. (2010) Treatment with a sphingosine analog does not alter the outcome of a persistent virus infection. Virology. 2010 Feb 20;397(2):260-9. Epub 2009 Dec 3. PMID: 19962171

27. Avni, D., M. Goldsmith, O. Ernst, R. Mashiach, T. Tuntland, M. Meijler, N. Gray, H. Rosen, and T. Zor. (2009) Modulation of TNFα, IL-10 and IL-12p40 levels by a ceramide-1-phosphate analog, PCERA-1, in vivo and ex vivo in primary macrophages. Immunol. Lett.

28. Goldsmith, M., D. Avni, G. Levy-Rimler, R. Mashiach, O. Ernst, M. Levi, B. Webb, M. Meijler, N. Gray, H. Rosen, and T. Zor. (2009) A ceramide-1-phosphate analogue, PCERA-1, simultaneously suppresses tumour necrosis factor-alpha and induces interleukin-10 production in activated macrophages. Immunology 127:103-115. PMID: 18793216

29. Cahalan, S, Rosen, H (2009) S1P1 signaling just keeps going and going and going… Nature Chem. Biol. 5(6):377-8

30. Bachovchin, D et al (2009) Identification of selective inhibitors of uncharacterized enzymes by high-throughput screening with fluorescent activity-based probes. 27:387-94. PMID: 19329999

31. Marsolais D, Rosen H (2009) Chemical modulators of S1P receptors as barrier-oriented therapeutic molecules. Nature Rev Drug Disc. 8(4):297-307 PMID: 19300460

32. Marsolais D, Hahm B, Walsh KB, Edelmann KH, McGavern D, Hatta Y, Kawaoka Y, Rosen H, Oldstone MBA. (2009) A critical role for the sphingosine analog AAL-R in dampening the cytokine response during influenza virus infection. PNAS 106(5):1560-5. PMID: 19164548

33. Rosen H and Don A. (2009) A lipid binding domain in sphingosine kinase 2. Biochemical and Biophysical Research Communications 380(1):87-92. PMID: 19168031

34. Niessen F, Furlan-Freguia C, Fernández JA, Mosnier LO, Castellino FJ, Weiler H, Rosen H, Griffin JH, Ruf W (2009) Endogenous EPCR/aPC-PAR1 Signaling prevents Inflammation-induced Vascular Leakage and Lethality. Blood 113(12):2859-66 PMID: 19141861

35. Fazilleau N, McHeyzer-Williams LJ, Rosen H, McHeyzer-Williams MG. (2009) The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding. Nature Immunology 10(4):375-84 PMID: 19252493

36. Nakai R, Salisbury CM, Rosen H, Cravatt BF. (2009) Ranking the selectivity of PubChem screening hits by activity-based protein profiling: MMP13 as a case study. Bioorg Med Chem 17(3):1101-8.. PMID: 18364257

37. Rosen H, Gonzalez-Cabrera PJ, Sanna MG, Brown S. (2008) Sphingosine 1-phosphate receptor signaling. Annual Rev Biochem 78:743-68

38. Gonzalez-Cabrera PJ, Jo E, Sanna MG, Brown S, Leaf N, Marsolais D, Schaeffer MT, Chapman J, Cameron M, Guerrero M, Roberts E, Rosen H. (2008) Full pharmacological efficacy of a novel S1P1 agonist that does not require S1P-like head-group interactions. Mol Pharm (75(5):1308-18)

39. Schurer SC, Brown SJ, Gonzalez-Cabrera P, Schaeffer MT, Chapman J, Chase P, Spicer T, Hodder P, Rosen H. (2008) Ligand-binding pocket shape differences between S1P₁ and S1P₃ determine efficiency of chemical probe identification by uHTS. Amer Chem Soc Chem Biol 3(8):486-498

40. Marsolais D, Hahm B, Edelmann KH, Walsh KB, Guerrero M, Hatta Y, Kawaoka Y, Roberts E, Oldstone MBA, Rosen H. (2008) Local not systemic modulation of dendritic cell S1P receptors in lung blunts virus-specific immune responses to influenza. Mol Pharm 74(3):896-903

41. Rosen H, Gonzalez-Cabrera P, Marsolais D, Cahalan S, Don AS, Sanna MG. (2008) Modulating tone: the overture of S1P receptor immunotherapeutics. Immunol Rev 223: 221-235

42. Nakai R, Salisbury CM, Rosen H, Cravatt BF. (2008) Ranking the selectivity of PubChem screening hits by activity-based protein profiling: MMP13 as a case study. Bioorg Med Chem 17(3):1101-8

43. Don AS, Rosen H. (2008) A fluorescent plate reader assay for ceramide kinase. Anal Biochem 375(2):265-71

44. Niessen F, Schaffner F, Furlan-Freguia C, Pawlinski R, Bhattacharjee G, Chun J, Derian CK, Andrade-Gordon P, Rosen H, Ruf W (2008) Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammation. Nature (In Press)

45. Tsukada YT, Sanna MG, Rosen H, Gottlieb RA (2007) S1P1-Selective agonist SEW2871 exacerbates reperfusion arrhythmias. J Cardiovasc Pharmacol50(6):660-669

46. Lee H-K, Brown SJ, Rosen H, Tobias PS (2007) Application of â-Lactamase Enzyme Complementation to the High-Throughput Screening of Toll-Like Receptor Signaling Inhibitors. Am Soc Pharm Exp Ther 72(4):868-75

47. Don AS, Martinez-Lamenca C, Webb WR, Proia RL, Roberts E, Rosen H (2007) Essential requirement for sphingosine kinase 2 in a sphingolipidapoptosis pathway activated by FTY720 analogs. J Biol Chem 282(21):15833-42

48. Gonzalez-Cabrera P, Hla T, Rosen H (2007) Mapping pathways downstream of sphingosine 1-phosphate subtype 1 by differential chemical perturbation and proteomics. J Biol Chem 282(10):7254-64

49. Rosen H, Sanna MG, Cahalan S, Gonzalez-Cabrera P (2007) Tipping the gatekeeper: S1P regulation of stromal barrier function. Trends Immunol. 28(3):102-7

50. Chun, J. and H. Rosen (2006). "Lysophospholipid receptors as potential drug targets in tissue transplantation and autoimmune diseases." Curr. Pharm. Des. 12(2):161-71.

51. Sanna MG, Wang SK, Gonzalez-Cabrera P, Don A, Marsolais D, Jo E, Cheng WC, Wong CH, Rosen H. (2006) Enhancement of capillary leakage and restoration of lymphocyte egress by a chiral S1P1 antagonist in vivo. Nat. Chem. Biol. 2(8):434-441

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NATURE CHEMICAL BIOLOGY NEWS & VIEWS: Schultz C: Lipid-induced phenotypes
NIMH Review: New Tool Can Boost or Block the Body’s Protective Inner Barriers

52. Alfonso C, McHeyzer-Williams MG, Rosen H. (2006) CD69 down-modulation and inhibition of thymic egress by short and long-term selective chemical agonism of S1P1 receptors. Eur J Immunol 36(1):149-59

53. Wei SH, Rosen H, Matheu MP, et al. (2005) Sphingosine 1-phosphate type 1 receptor agonism inhibits transendothelial migration of medullary T cells to lymphatic sinuses. Nat Immunol 6:1228-35

NATURE IMMUNOLOGY NEWS & VIEWS: Ley K, Morris M: Signals for lymphocyte egress

54. Rosen H, Goetzl EJ. (2005) Sphingosine 1-phosphate and its receptors: an autocrine and paracrine network. Nat Rev Immunol. 5(7):560-570

55. Jo E, Sanna MG, Gonzalez-Cabrera PJ, Thangada S, Tigy G, Osborne DA, Hla T, Parrill AL, Rosen H. (2005) S1P1-selective in vivo-active agonist from high throughput screening: Off-the-shelf chemical probes of receptor interactions, signaling and fate. Chem Biol 12(6):703-715

TSRI News & Views: July 4, 2005 Scientists Discover New Key to Pulmonary Edema in Respiratory Distress Syndrome

56. Martinez X, Kreuwel HTC, Redmond WL, Trenney R, Hunter K, Rosen H, Sarvetnick N, Wicker LS, Sherman LA. (2005) CD8+ T cell tolerance in NOD mice is restored by insulin dependent diabetes (Idd) resistance alleles. J. Exp. Med. 175(3):1677-1685

57. Rosen H. (2005) Chemical approaches to the lysophospholipid receptors. Prostaglandins & Other Lipid Mediators. 77(1-4):179-184

58. Gong Q, Ou Q, Ye S, Lee WP, Cornelius J, Diehl L, Lin WY, Hu Z, Lu Y, Chen Y, Wu Y, Meng YG, Gribling P, Lin Z, Nguyen K, Tran T, Zhang Y, Rosen H, Martin F, Chan AC. (2005) Importance of cellular microenvironment and circulatory dynamics in B cell immunotherapy. J. Imm. 174(2):817-26

59. Goetzl E.J., Rosen H. (2004) Regulation of immunity by lysosphingolipids and their G protein-coupled receptors. J Clin Invest. 2004 Dec;114(11):1531-7

60. Yan L, Hale JJ, Lynch CL, Budhu R, Gentry A, Mills SG, Hajdu R, Keohane CA, Rosenbach MJ, Milligan JA, Shei GJ, Chrebet G, Bergstrom J, Card D, Rosen H, Mandala SM. Design and synthesis of conformationally constrained 3-(N-alkylamino)propylphosphonic acids as potent agonists of sphingosine-1-phosphate (S1P) receptors. Bioorg Med Chem Lett. 2004 Oct 4;14(19):4861-6.

61. Hale JJ, Yan L, Neway WE, Hajdu R, Bergstrom JD, Milligan JA, Shei GJ, Chrebet GL, Thornton RA, Card D, Rosenbach M, Rosen H, Mandala S. Synthesis, stereochemical determination and biochemical characterization of the enantiomeric phosphate esters of the novel immunosuppressive agent FTY720. Bioorg. Med. Chem. 2004 Aug 6; 12:4803-7

62. Hale, J., Doherty, G., Toth, L., Mills, S., Hajdu, R., Keohane, C., Rosenbach, M., Milligan, J., Shei, G., Chrebet, G., Bergstrom, J., Card, D., Forrest, M., Sun, S., West, S., Xie, H., Nomura, N, Rosen, H., Mandala, S. (2004) Selecting against S1P3 enhances the acute cardiovascular tolerability of 3-(N-benzyl)aminopropylphosphonic acid S1P receptor agonists. Bio. Med. Chem. Ltrs. (in press)

63. Hale, J., Doherty, G., Toth, L., Li, Z., Mills, S., Hajdu, R., Keohane, C., Rosenbach, M., Milligan, J., Shei, G., Chrebet, G., Bergstrom, J., Card, D., Rosen, H., Mandala, S. (2004) The discovery of 3-(N-alkyl)aminopropylphosphonic acids as potent S1P receptor agonists. Bio. Med. Chem. Ltrs. (in press)

64. Hale, J., Neway, W., Mills, S., Hajdu, R., Keohane, C., Rosenbach, M., Milligan, J., Shei, G., Chrebet, G., Bergstrom, J., Card, D., Koo, G., Koprak, S., Jackson, J., Rosen, H., Mandala, S. (2004) Potent S1P receptor agonists replicate the pharmacologic actions of the novel immune modulator FTY720. Bio. Med. Chem. Ltrs. 14:3351-3355

65. Forrest, M., Sun, S.-Y., Hajdu, R., Bergstrom, J., Card, D., Doherty, G., Hale, J., Keohane, C., Meyers, C., Milligan, J., Mills, S., Nomura, N., Rosen, H.,Rosenbach, M., Shei, G.-J., Singer, I., Tain, M., West, S., White, V., Xie, J., Proia, R., Mandala, S. (2004) Immune cell regulation and cardiovascular effects of sphingosine 1-phosphate receptor agonists in rodents are mediated via distinct receptor sub-types. J. Pharmacol. Exp. Therap. 309:758

66. Sanna, G., Liao, J., Jo, E., Alfonso, C., Ahn, M., Peterson, M., Webb, B., Lefebvre, S., Chun, J., Gray, N., Rosen, H. (2004) Spingosine 1-phosphate (S1P) receptor subtypes S1P1 and S1P3, respectively, regulate lymphocyte recirculation and heart rate. J. Biol. Chem. 279:13839-13848

67. Rosen, H., Alfonso, C., Surh, C.D., McHeyzer-Williams, M.G. (2003) Rapid induction of medullary thymocyte phenotypic maturation and inhibition ofthymic egress at low nanomolar concentrations of sphingosine 1-phosphate receptor agonist. PNAS 100:10907-10912.

68. Rosen, H., Sanna, G., Alfonso, C. (2003) Egress: a receptor-regulated step in lymphocyte trafficking. Imm. Rev. 195:160-177.

69. Rosen, H., Kozarich, J.W. (2003) Next-generation therapeutics. Balancing on the uneasy cusp: integrating linear and parallel approaches to new generation therapeutics. Curr. Opin. Chem. Biology 7:443-445.

70. Rosen, H., Liao, J. (2003) Sphingosine 1-phosphate pathway therapeutics: a lipid ligand-receptor paradigm. Curr. Opin. Chem. Bio. 7:461-468.

71. J. Xie, N. Nomura, E. Quackenbush, M. Forrest and H. Rosen (2003) Sphingosine-1-Phosphate Receptor Agonists Impair the Efficiency of the Local Immune Response by Altering Trafficking of Naïve and Antigen-Activated CD4+ T Cells. J. Immunol. 170: 3662-3670.

72. Parent, S.A., T. Zhang, G. Chrebet, J.A. Clemas, D.J. Figueroa, B. Ky, R.A. Blevins, C.P. Austin, and H. Rosen. (2002). Molecular characterization of the murine SIGNR1 gene encoding a C-type lectin homologous to human DC-SIGN and DC-SIGNR. Gene 293:33.

73. Mandala, S., Hajdu, R., Bergstrom, J., Quackenbush E., Xie J., Milligan, J., Thornton, R., Shei, G-J., Card, D., Keohane, C., Rosenbach, M., Hale, J., Lynch, C., Rupprecht, K., Parsons, W., and Rosen, H. (2002) Alteration of Lymphocyte Trafficking by Sphingosine 1-Phosphate Receptor Agonists. Science 296:346-9. Published online 28 March 2002;10.1126/science.1070238

74. Beck, L.A. et al. (1997). Cutaneous injection of RANTES causes eosinophil recruitment: comparison of non-allergic and allergic human subjects. JournalImmunol. 159: 2962-2972.

75. Zheng, H., D. Fletcher, W. Kozak et al (1995). Resistance to fever induction and impaired acute phase response in Interleukin-1? deficient mice. Immunity 3:9-19.

76. Gordon, E.J., K.J. Myers, J.P.Dougherty, H. Rosen and Y. Ron. (1995) “Both anti-CD11a and anti-CD11b therapy delay the onset and diminish the severity of experimental autoimmune encephalitis. Neuroimmunol. Nov; 62(2): 153-60

77. Konteatis Z, Siciliano S, VanRiper G, et al. (1994) Development of C5a receptor antagonists: differential loss of functional responses. J. Immunol. 153:4200-5.

78. DeMartino, J., G. Van Riper, S. Siciliano, Z. Konteatis, H. Rosen and M. Springer 1994 “The amino-terminus of the human C5a receptor is required for high affinity C5a binding but not for signal transduction.” J.Biol.Chem. 269:14446-50.

79. Van Riper, G., D. Nicholson, M. Scheid, P. Fischer, M. Springer and H. Rosen 1994 “Induction, characterization and functional coupling of the high affinitychemokine receptor for Rantes and MIP-1a upon differentiation of an eosinophilic HL-60 line.” J.Immunol. 152: 4055-61.

80. Meurer, R., G. Van Riper, W. Feeney, P. Cunningham, D. Hora, M. Springer, D. E. MacIntyre and H. Rosen 1993 “Rapid formation of eosinophilicintradermal inflammatory sites in the dog by injection of human Rantes but not hMCP-1, hMIP-1a or hIL-8.” J.Exp.Med. 178:1913-21

81. Siciliano, S., J. DeMartino, Z. Konteatis, L. Malkowits, G. Van Riper, T. Rollins, S. Bondy, H. Rosen and M. Springer 1994 “Two-site binding of C5a by its receptor: A new binding paradigm for G protein-coupled receptors.” Proc. Natl. Acad. Sci. U.S.A. 91: 1214-8.

82. Koo, G., H. Rosen, A. Sirotina, X.-D. Ma and L. Shultz 1993 “Prevention of immunopathologic changes in viable motheaten bone marrow chimeras with anti-CD11b antibody.” J. Immunol. 151: 6733-41.

83. Van Riper, G., S. Siciliano, P.A. Fischer, R. Meurer, M. Springer, and H. Rosen. 1993. "Characterization and species distribution of high affinity GTP-coupled receptors for human Rantes and monocyte-chemoattractant protein-1". J. Exp. Med. 177: 851-6.
21) Cervia,J.S., H.Rosen and H.W.Murray. (1993) "Effector role of the blood monocyte in experimental visceral Leishmaniasis." Infection and Immunity 61: 1330-3.

84. Whitcup,S., L.R.DeBarge, H.Rosen, R.B.Nussenblatt and C-C. Chan. (1993) "Monoclonal antibody against CD11b/CD18 inhibits endotoxin-induceduveitis." Invest. Ophth. Vis. Sci. 34(3):673-681

85. Piguet, P.F., H. Rosen, C. Vesin and G.E.Grau (1993) "Antibody to the leukocyte integrins CD11a or CD11b prevents or cures pulmonary fibrosis elicited in mice by bleomycin or silica." Am. Rev.Resp.Dis. 147: 445-451

86. Al Attiyah, R, H. Rosen and G.A.W. Rook. (1992) "A model for the investigation of factors influencing TNF-mediated haemorrhagic necrosis in tissue sites primed with mycobacterial antigen preparations." Clin.Exp.Immunol. 88: 537-42.

87. Mielke, M., H. Rosen, S. Brocke, C. Peters and H. Hahn (1992) “Protective immunity and granuloma formation are mediated by two distinct TNF-alpha and Gamma Interferon-dependent T cell-phagocyte interactions in murine listeriosis: Dissociation on the basis of Phagocyte adhesion mechanisms.” Infect. Immun. 60: 1875-82.

88. Robinson MK, D Andrew, H Rosen, D Brown, S Ortlepp, P Stephens and EC Butcher (1992) "Antibody against the Leu-CAM ?-chain (CD18) promotes both LFA-1- and CR3-dependent adhesion events." J.Immunol 148:1080-5.

89. Grau G., P. Pointaire, P.-F. Piguet, H. Rosen, I. Stamenkovic, F. Takei and P. Vassalli (1991). “Late administration of monoclonal antibody to leukocyte function-antigen abrogates incipient murine cerebral malaria.” Eur.J.Immunol. 21: 2265-7.

90. Blankenstein, T., Z. Qin, K. Uberla, W. Muller, H. Rosen, D.-T. Volk and T. Diamantstein (1991). “Tumor suppression after tumor cell targeted TNF alpha gene transfer.” J.Exp.Med.173: 1047-52.

91. Hutchings, P., H. Rosen, L. O'Reilly, E. Simpson, S. Gordon and A. Cooke (1990). “Transfer of diabetes in mice prevented by blockade of adhesion-promoting receptor on macrophages.” Nature. 348: 639-42.

92. Rosen, H. and S. Gordon (1990). “Adoptive transfer of fluorescence-labelled cells shows that resident peritoneal macrophages are able to migrate into specialized lymphoid organs and inflammatory sites in the mouse.” Eur.J.Immunol. 20: 1251-8.

93. Lunn, E., V. Perry, M. Brown, H. Rosen and S. Gordon (1989). “Absence of Wallerian degeneration does not hinder regeneration in peripheral nerves.”Eur.J.Neurosci. 1: 27-33.

94. Rosen, H., S. Gordon and R. North (1989). “Exacerbation of murine listeriosis by a monoclonal antibody specific for the type 3 complement receptor ofmyelomonocytic cells.” J.Exp.Med. 170: 27.

95. Rosen, H., G. Milon and S. Gordon (1989). “Antibody to the murine type 3 complement receptor inhibits T lymphocyte-dependent recruitment ofmyelomonocytic cells in vivo.” J.Exp.Med. 169: 535.

96. Cooper, A., H. Rosen and J. Blackwell (1988). “Monoclonal antibodies that recognize distinct epitopes of the macrophage type 3 complement receptordiffer in their ability to inhibit the binding of Leishmania promastigotes harvested at different phases of their growth cycle.” Immunology. 65: 511-4.

97. Rosen, H. and S. Gordon (1987). “Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo.” J.Exp.Med. 166: 1685-1702.

98. Rosen, H. and S. Gordon (1987). “Surface events in polymorphonuclear leukocyte activation: insights from a hydrophobic membrane antigen that triggers the respiratory burst.” J.Cell Sci. 88: 591-601.

99. Berton, G., H. Rosen, R. Ezekowitz, P. Bellavite, M. Serra, F. Rossi and S. Gordon (1986). “Monoclonal antibodies to a particulate superoxide-forming system stimulate a respiratory burst in intact guinea pig neutrophils.” Proc.Natl.Acad.Sci.U.S.A. 83: 4002-6.

100. Millar, R., H. Rosen, M. Badminton, C. Pesqualini and B. Kerdelhue (1983). “Luteinizing-hormone releasing-hormone binding to purified rat pituitary nuclei.” FEBS Lett. 153: 382-6.

Infectious Diseases

1. Bowman, J.C., P.S. Hicks, M.B. Kurtz, H. Rosen, D.M. Schmatz, P.A. Liberator, and C.M. Douglas. 2002. The Antifungal Echinocandin CaspofunginAcetate Kills Growing Cells of Aspergillus fumigatus In Vitro. Antimicrob. Agents Chemother. 46: 3001.

2. Abruzzo, G.K., Gill, C.J., Flattery, A.M., Kong, L., Leighton, C., Smith, J.G., Pikounis, V.B., Bartizal, K., and H. Rosen. (2000). Efficacy of the echinocandinCaspofungin against aspergillosis and candidiasis in cuyclophosphamide-induced immunosuppressed mice. Antimicrob. Agents Chemother. 44:2310-8.

3. Kohler, J., K.L. Dorso, K. Young, G.G. Hammond, H. Rosen, H. Kropp and L.L. Silver (1999) In vitro activities of the potent, broad-spectrum carbapenemMK-0826 against broad-spectrum ?-lactamase- and Extended-spectrum ?-lactamase-producing Klebsiella pneumoniae and Escherichia coli clinical isolates.Antimicrob. Agents Chemother. 43:1170-6.

4. Rosen, H., R. Hajdu, L. Silver, H.Kropp, K. Dorso, J. Kohler, J. G. Sundelof, J. Huber, G. G. Hammond, J. J. Jackson, C. J. Gill, R. Thompson, B. A. Pelak, J. H. Epstein-Toney, G. Lankas, R. R. Wilkening, K. J. Wildonger, T. A. Blizzard, F. P. DiNinno, R. W. Ratcliffe, J. V. Heck, J. W. Kozarich, M. L. Hammond (1999) Reduced Immunotoxicity and Preservation of Antibacterial Activity in a Releasable Side-Chain Carbapenem Antibiotic. Science 283:703-6.

5. Gill, C.J., J.J. Hackson, L.S. Gerckens, B.A. Pelak, R.K. Thompson, J.G. Sundelof, H. Kropp and H. Rosen. (1998) In vivo activity and phramacokineticevaluation of a novel long-acting carbapenem MK-826. Antimicrob. Agents. Chemother. 42:1996-2001.

6. Abruzzo, G.K., A.M. Flattery, C.J. Gill, L. Kong, J.G. Smith, V. Pikounis, J. Balkovec, A. Bouffard, J. Dropinski, H. Rosen, H. Kropp & K. Bartizal. (1997) Evaluation of the echinocandin antifungal MK-0991 efficacies in mouse models of disseminated aspergillosis, candidiasis and cryptococcosis. Antimicrob. Agents. Chemother. 41:2333-8.

7. Sundelof, J.G., R. Hajdu, C.J. Gill. R. Thompson, H. Rosen and H. Kropp. (1997) Pharmacokinetics of L-749,345, a long-acting carbapenem antibiotic in primates. Antimicrob. Agents. Chemother. 41:1743-8.