Min Guo, Ph.D.
Education
Ph.D., Structural Biology, University of Science and Technology of China (2005
B.S., Biology, University of Science and Technology of China (2000)
Current Research
Nature appears to exert great effort to prevent toxic mistranslation from confusing serine or glycine for alanine by having multiple editing checkpoints. My research is trying to understand the chemical basis of this confusion, and the mechanism of the following proofreading step using x-ray crystallography, bioinformatics and biochemical assays. My another great interest is about the structural mechanism of non-canonical functions of human aaRSs, and their dynamic regulations.
Publications:
1. Guo M, Shapiro R, Schimmel P, Yang XL. Introduction of a leucine half-zipper engenders multiple high quality crystals of a recalcitrant tRNA synthetase. 2009 submitted.
2. Guo M, Chong YE, Shapiro R, Beebe K, Yang XL, Schimmel P. Mistranslation from serine paradox caused by AlaRS recognition dilemma. Nature, 2009 in press
3. Zhou Q, Kapoor M, Guo M, Belani R, Xu X, Kiosses WB, Hanan M, Park C, Armour E, Do MH, Nangle LA, Schimmel P, Yang XL. Virtual substrate presentation by VE-cadherin captures human tRNA synthetase for angiostatic function. Nature Struct. Mol. Biol., 2009 in press
4. Guo RT, Chong YE, Guo M, Yang XL. Crystal structures and biochemical analyses suggest unique mechanism and role for human GlyRS in Ap4A homeostasis. J. Biol. Chem., 2009 in press
5. Guo M, Chong YE, Beebe K, Shapiro R, Yang XL, Schimmel P. The C-Ala domain brings together editing and aminoacylation functions on one tRNA. Science, 2009 Aug 7; 325;744-7
6. Schimmel P, Guo M. A tipping point for mistranslation and disease. Nat. Struct. Mol. Biol., 2009 Apr 1;16(4): 348-9
7. Schimmel P, Guo M. Ancient tRNA Synthetase Meets Modern Structural Biology. Structure, 2009 Mar 11;17: 315-7
8. Guo M, Ignatov M, Musier-Forsyth K, Schimmel P, Yang XL. Crystal structure of tetrameric form of human lysyl-tRNA synthetase: Implications for multisynthetase complex formation. Proc. Natl. Acad. Sci. USA, 2008 Feb 19;105(7):2331-6.
9. Guo M, Xu F, Yamada J, Egelhofer T, Gao Y, Hartzog GA, Teng M, Niu L. Core Structure of the Yeast Spt4-Spt5 Complex: A Conserved Module for Regulation of Transcription Elongation. Structure, 2008 Nov 12;16:1649-1658.
10. Beebe K, Waas W, Druzina Z, Guo M, Schimmel P. A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Anal Biochem., 2007 Sep 1;368(1):111-21.
11. Yang XL, Guo M, Kapoor M, Ewalt KL, Otero FJ, Skene RJ, McRee DE, Schimmel P. Functional and crystal structure analysis of active site adaptations of a potent anti-angiogenic human tRNA synthetase. Structure, 2007 Jul;15(7):793-805.
12. Fang P, Wang J, Li X, Guo M, Xing L, Cao X, Zhu Y, Gao Y, Niu L, Teng M. Crystallization and preliminary X-ray analysis of Escherichia coil Rnase G. Acta Cryst. F, 2009 Jun 1; 65:586-8.
13. Li X, Guo M, Fan J, Tang W, Wang D, Ge H, Rong H, Teng M, Niu L, Liu Q, Hao Q. Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases. Protein Sci., 2006 Apr;15(4):761-73.
14. Guo M, Teng M, Niu L, Liu Q, Huang Q, Hao Q. Crystal structure of cysteine-rich secretory protein stecrisp reveals the cysteine-rich domain has a K+-channel inhibitor-like fold. J. Biol. Chem., 2005 Apr; 280: 12405 – 12412.
15. Wang D, Guo M, Liang Z, Fan J, Zhu Z, Zang J, Zhu Z, Li X, Teng M, Niu L, Dong Y, and Liu P. Crystal structure of human vacuolar protein sorting protein 29 reveals a phosphodiesterase/nuclease-like fold and two protein-protein interaction sites. J. Biol. Chem.,2005 Jun; 280: 22962 – 22967.
16. Wang J, Guo M, Tu X, Zheng D, Teng M, Niu L, Liu Q, Huang Q, Hao Q. Purification, partial characterization, crystallization and preliminary X-ray diffraction of two cysteine-rich secretory proteins from Naja atra and Trimeresurus stejnegeri venoms , Acta Cryst. D, 2004; 60: 1108-1111.
17. Fan J, Wang D, Liang Z, Guo M, Teng M, Niu L. Maize uroporphyrinogen III methyltransferase: overexpression of the functional gene fragments in Escherichia coli and one-step purification. Protein Expr Purif., 2006 Mar;46(1):40-6.
18. Wang J, Shen B, Guo M, Lou X, Duan Y, Cheng XP, Teng M, Niu L, Liu Q, Huang Q, Hao Q. Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel. Biochemistry, 2005 Aug 2;44 (30):10145-52.
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