Histology

The Histology Core Laboratory is a full service lab in the area of histological studies. We are here to assist you with all your histological needs. If you have questions about a project, a staining method or proper techniques, we can answer all those questions. Before starting a project, please contact us so that we can make your life easier and help you plan a course of action. Drop into the lab, call or email for more information.

  • Identification of tissue specimens, fixation techniques including a supply of fixatives for use, proper processing methods
  • Paraffin blocking of specimens with attention to correct orientation
  • Cutting of paraffin blocks including specific site locations
  • Routine histology stains to advanced staining methods
  • Cryostat sectioning and staining
  • Decalcification of bone specimens
  • Knife sharpening

 

Digital Slide Scanner Server

For access to the Digital Slide Scanner Server, please click here.

Contact

The Scripps Florida Histology Core (SFHC) is able to provide full histological services as well as technical support for investigators. The lab is equipped with the appropriate ventilated working areas and equipment to prepare, process and stain frozen or paraffin embedded specimens.

Tina Van Meter, HT (ASCP), is a nationally certified histotechnician and has 37 years of experience in various histological methods that are utilized in research laboratories. Her primary focus has been in special stains, immunohistochemistry/immunofluorescence and electron microscopy techniques.

The services are provided to the research community at Scripps Florida at a reduced cost.

The Histology Core is located in Building B, Room #244C

Office hours are Monday, Wednesday, Friday, 4:00 pm - 5:00 pm

If you cannot make those hours, please email Tina Van Meter to schedule an appointment.

The Histology Core contains the following instrumentation: 

  • VIP Tissue Processor
  • BIOCARE NxGen Decloaking Chamber
  • Rotary Microtome
  • Staining Center
  • Leica Cryostat
  • Zeiss Axio Microscope/Camera Software
  • Leica Bond-Max Immunostainer
  • Leica Autostainer XL
  • General Data Laser Cassette Marker

 

Please contact Tina Van Meter at (561) 228-2469 to reserve the Leica Cryostat and Zeiss Axio Microscope.

Special Stains:

The Core can provide a wide range of special stains. Below is a sample of special stains that we offer. Additional stains are available upon request. Please contact us for a free consultation.

  • Congo Red
  • Crystal Violet
  • Gram Stain
  • Luxol Fast Blue
  • Masson Trichrome
  • Oil Red O
  • Periodic Acid Schiff (PAS)
  • Safronin O

 

Immunohistochemistry:

The Core offers optimization of antibodies and protocols and double and triple staining techniques for immnuohistochemistry/immunofluorescence. Please contact us for a free consultation.

  • Fixation techniques
  • Tissue processing
  • Embedding
  • Paraffin sectioning
  • H&E's
  • Special stains
  • Immunohistochemistry/immunofluorescence
  • Cryostat embedding and sectioning
  • Decalcification of bone specimens
Core users please make an appointment for a free consultation with Tina Van Meter, (561) 228-2469, in advance of a project to discuss tissue preparation, necessary controls and scope of work to maximize the economy and success of the project.

Contacts