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The Gerace Laboratory


2000 - Present
1991 - 1999 
1981 - 1990
1978 - 1980

2000 - Present

Tapia, O, Gerace L. (2016).  Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation.  Methods Mol Biol 1411:177-194. PubMed

Tapia O, Fong LG, Huber MD, Young SG, Gerace L. (2015). Nuclear Envelope Protein Lem2 is Required for Mouse Development and Regulates MAP and AKT Kinases. PLoS 19;10(3):e0116196. PubMed

Cassany A, Ragues J, Guan T, Bégu D, Wodrich H, Kann M, Nemerow GR, Gerace L. (2014). Nuclear import of adenovirus DNA involves direct interation of hexon with an N-terminal domain of the nucleoporin Nup214. J. Virol. pii: JVI.02639-14. PubMed

Banerjee l, Zhang J, Moore-Morris T, Pfeiffer E, Buchholz KS, Liu A, Quyang K, Stroud MJ, Gerace L, Evans SM, McCulloch A, Chen J. (2014).  Targeted ablation of nesprin 1 and nesprin 2 from murine myocardium results in cardiomyopathy, altered nuclear morphology and inhibition of the biomechanical gene response.  PLoS Genet 10(2):e1004114. PubMed

Huber MD, Vesely P, Datta K and Gerace L. (2013) Erlins restrict SREBP activation in the ER and regulate cholesterol homeostasis. J Cell Biol 203:427-36 PubMed

Naji, S., Ambrus, G., Cimermančič, P., Reyes, J.R., Johnson, J.R., Filbrandt, R., Huber, M.D. Vesely, P., Krogan, N.J., Yates III, J.R., Saphire, A.C. and Gerace, L.  (2012) Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol. Cell Proteomics, 11(4):M111.015313. PubMed

Gerace, L. and Huber, M. (2012) Nuclear lamina at the crossroads of the cytoplasm and nucleus.  J. Struct. Biol., 177(1):24-31. PubMed

Kerkow DE, Carmel AB, Menichelli E, Ambrus G, Hills RD Jr, Gerace L, Williamson JR. (2012). The structure of the NXF2/NXT1 heterodimeric complex reveals the combined specificity and versatility of the NTF2-like fold. J Mol Biol. 415(4):649-65. PubMed

Edgcomb, S.P., Carmel, A.B., Naji, S, Ambrus-Aikelin, G, Reyes, J.R., Saphire, A.C., Gerace, L. and Williamson, J.R. (2012) DDX1 Is an RNA-Dependent ATPase Involved in HIV-1 Rev Function and Virus Replication. J Mol Biol., 415(1):61-74.PubMed

Ambrus-Aikelin G, Whitby LR, Singer EL, Trott O, Choi E, Olson AJ, Boger DL, Gerace L. (2010). Small molecule peptidomimetic inhibitors of importin a/b mediated nuclear transport. Bioorg Med Chem., 18(21):7611-20. PubMed

Hintersteiner M, Ambrus-Aikelin G, Bednenko J, Schmied M, Gstach H, Seifert J, Singer E, Gerace L, Auer M. (2010). Identification of a small molecule inhibitor of importin beta mediated nuclear import by confocal on-bead screening of tagged one-bead one-compound libraries. ACS Chem Biol. PubMed

Datta, K., Guan, T., Gerace, L. (2009). NET37, a member of family 31 glycosidases localized to the nuclear envelope, is required for myoblast differentiation. J Biol Chem, 284(43):29666-76. PubMed

Huber, M., Guan, T., Gerace, L. (2009) Overlapping functions of nuclear membrane proteins NET25 (Lem2) and emerin in regulation of ERK signaling in myoblast differentiation. Mol Cell Biol. 29(21):5718-28. PubMed 

Ben-Efraim, I., Frosst, P.D., Gerace, L. (2009) Karyopherin binding interactions and nuclear import mechanism of nuclear pore complex protein Tpr. BMC Cell Biol. 10:74. PubMed
Wang P, Liu GH, Wu K, Qu, J, Huang B, Zhang X, Zhou X, Gerace L, Chen C. (2009) Repression of classical nuclear export by S-nitrosylation of CRM1. J Cell Sci. 122(Pt 20):3772-9. PubMed
Liu GH, Gerace L. (2009) Sumoylation regulates nuclear localization of lipin-1alpha in neuronal cells. PLoS One. 4(9):e7031. PubMed

Cassany A, Gerace L. (2009) Reconstitution of nuclear import in permeabilized cells. Methods Mol Biol. 464:181-205. PubMed
Ostlund C, Guan T, Figlewicz DA, Hays AP, Worman HJ, Gerace L, Schirmer EC. (2009) Reduction of a 4q35-encoded nuclear envelope protein in muscle differentiation. Biochem Biophys Res Commun. 389(2):279-83. PubMed
Datta, K., Guan, T., Gerace, L. (2009) NET37, a nuclear envelope transmembrane protein with glycosidase homology, is involved in myoblast differentiation.  J Biol Chem. 284(43):29666-76.PubMed
Liu GH, Guan T, Datta K, Coppinger J, Yates J 3rd, Gerace L. (2009) Regulation of myoblast differentiation by the nuclear envelope protein NET39. Mol Cell Biol. 29(21):5800-12. PubMed
Smith JG, Cassany A, Gerace L, Ralston R, Nemerow GR. (2008) Neutralizing antibody blocks adenovirus infection by arresting microtubule-dependent cytoplasmic transport. J Virol. 82:6492-500.PubMed
Edgcomb S.P., Aschrafi A., Kompfner E., Williamson J.R., Gerace L., Hennig M. (2008) Protein structure and oligomerization are important for the formation of export-competent HIV-1 Rev-RRE complexes. Protein Sci. 2008 Mar;17(3):420-30. PubMed
Huber, M. D., Gerace, L. (2007)  The size-wise nucleus: nuclear volume control in eukaryotes.   J Cell Biol. 179:583-4. PubMed
Chen IH., Huber M., Guan T., Bubeck A., Gerace, L. (2006) Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis.  BMC Cell Biology, 7:38. PubMed

Wodrich H., Cassany A., D’ Angelo MA., Guan T., Nemerow G., Gerace L. (2006) Adenovirus core protein pVII is translocated  into the nucleus by multiple import receptor  pathways. J. Virology, 80:9608-18. PubMed

Ospina JK, Gonsalvez GB, Bednenko J, Darzynkiewicz E, Gerace L, Matera AG. (2005) Cross-talk between snurportin1 subdomains. Mol Biol Cell, 16:4660-71. PubMed

Schirmer, E.C., Gerace, L. (2005) The nuclear membrane proteome: extending the envelope. Trends Biochem Sci, 10:551-8. PubMed

Wiethoff, C.M., Wodrich, H., Gerace, L., Nemerow, G.R. (2005) Adenovirus protein VI mediates membrane disruption following capsid disassembly. J. Virology, 79:1992-2000. PubMed

Ohba, T., Schirmer, E.C., Nishimoto, T., Gerace, L. (2004) Energy and temperature dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore. J. Cell Biol., 167:1051-62. PubMed

Schirmer, E.C., Gerace, L. (2004) The stability of the nuclear lamina polymer changes with the composition of lamin subtypes according to their individual binding strengths. J Biol Chem. Oct 279:42811-42817. PubMed
Gerace, L., (2004) TorsinA and torsion dystonia: Unraveling the architecture of the nuclear envelope. PNAS 101:8839-8840.  PubMed

Ben-Efraim, I., Zhou, Q., Wiedmer, T., Gerace, L., Sims, P.J. (2004) Phospholipid scramblase 1 is imported into the nucleus by a receptor-mediated pathway and interacts with DNA. Biochemistry 43:3518-3526. PubMed

Wodrich, H., Guan, T., Cingolani, G., von Seggern, D., Nemerow, G., Gerace, L. (2003) Switch from capsid protein import to adenovirus assembly by cleavage of nuclear transport signals. EMBO J 22:6245-6255. PubMed

Schirmer, E., Yates, J.R., Gerace, L. (2003) MudPIT: A Powerful Proteomics Tool for Discovery. Discov Med., 3:38-39. PubMed

Cingolani, G., Gerace, L. (2003) Molecular basis for nucleocytoplasmic transport. Handbook of Cell Signaling 3:419-429.

Schirmer, E., Florens, L., Guan, T., Yates, J.R., Gerace, L. (2003) Nuclear membrane proteins with potential disease links found by subtractive proteomics. Science 301:1380-1382. PubMed

Bednenko, J., Cingolani, G., Gerace, L. (2003) Importin beta contains a COOH-terminal nucleoporin binding region important for nuclear transport. J. Cell Biol. 162:391-401. PubMed

Bednenko, J., Cingolani, G., Gerace, L. (2003) Nucleocytoplasmic transport: Navigating the channel. Traffic 4:127-135. PubMed

Koerner, C., Guan, T., Gerace, L., and Cingolani, G. (2003) Synergy of silent and hot spot mutations in importin beta reveals a dynamic mechanism for recognition of a nuclear localization signal. J. Biol. Chem. 278:16216-16221. PubMed

Cingolani, G., Bednenko, J., Gillespie, M.T., Gerace, L.  (2002) Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta. Mol. Cell 10:1345-53. PubMed

Lyman, S.K., Guan, T., Bednenko, J., Wodrich, H. and Gerace, L. (2002) Influence of cargo size on Ran and energy requirements for  nuclear protein import.  J. Cell Biol. 159:55-67 (2002). PubMed

Kehlenbach, R.H. and Gerace, L. (2002) Analysis of nuclear protein import and export in vitro using fluorescent cargoes. Methods Mol. Biol. 189:234-45. PubMed
Frosst, P., Guan, T., Subauste, C., Hahn, K. and Gerace, L. (2002) Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export. J. Cell Biol. 156:617-630. PubMed

Schirmer, E.C. and Gerace, L. (2002) Organellar proteomics: the prizes and pitfalls of opening the nuclear envelope. Genome Biol. 3:1008.1-1008.4. PubMed

Lyman, S.K. and Gerace, L. (2001) Nuclear pore complexes: dynamics in unexpected places. J. Cell Biol. 154:17-20.   PubMed

Schmitt, I., and Gerace, L. (2001) In vitro analysis of nuclear transport mediated by the C-terminal shuttle domain of Tap. J. Biol. Chem. 276:42355-42363. PubMed

Schirmer, E.C., Guan, T. and Gerace, L. (2001) Involvement of the lamin rod domain in heterotypic lamin interactions important for nuclear organization. J. Cell Biol.153:479-489. PubMed
Ben-Efraim, I.  and Gerace, L.  (2001) Gradient of increasing affinity of importin b for nucleoporins along the pathway of nuclear import. J. Cell Biol. 152:411-417 PubMed
Kehlenbach, R.H., Assheuer, R., Kehlenbach, A., Becker, J. and Gerace, L. (2001) Stimulation of nuclear export and inhibition of nuclear import by a Ran mutant deficient in binding to RanBP1. J. Biol. Chem. 276:14524-14531. PubMed
Kehlenbach R.H. and Gerace, L. (2000) Analysis of nuclear protein import and export in vitro using fluorescent cargoes.  In Methods in Molecular Biology GTPase Protocols:  The Ras Superfamily.PubMed
Cingolani, G., Lashuel, H.A. , Gerace, L. and C.W. Muller  (2000) Nuclear import factors importin alpha and importin beta undergo mutually induced conformational changes upon association. FEBS Lett. 3:291-8. PubMed
Guan, T., Kehlenbach, R.H., Schirmer, E.C., Kehlenbach, A., Fan, F., Clurman, B., Arnheim,and Gerace, L. (2000)  Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export. Mol. Cell Biol. 20:5619-5630. PubMed

Saphire, A.C., Guan, T. , Schirmer, E.C., Nemerow, G.R. and Gerace, L. (2000) Nuclear import of adenovirus DNA in vitro involves the nuclear protein import pathway and hsc70. J. Biol. Chem. 275:4295-4304. PubMed
Kehlenbach, R.H. and Gerace, L. (2000) Phosphorylation of the nuclear transport machinery down-regulates nuclear  protein  import in  vitro. J. Biol. Chem. 275:17848-17856. PubMed