Xiaohua Wu, PhD
Professor
Department of Molecular and Cellular Biology
Research Focus
Gross chromosomal arrangements (GCRs) are hall markers of cancer cells, and DNA double-stand breaks (DSBs) are the major cause of cancer-associated genome instability. Unrepaired DSBs can lead to chromosomal breakage and cell death, while incorrectly repaired DSBs would lead to genome instability. The research interest of my laboratory is to understand the molecular mechanisms underlying the function of DNA damage checkpoint and DSB repair to maintain genome stability and prevent cancer and aging. We have established novel EGFP-based DSB repair assays and chromosomal translocation mouse models to investigate the mechanisms underlying DSB repair and chromosomal rearrangement. We are also interested in understanding the role of the breast cancer suppressor BRCA1, and other disease-linked and genome-stability maintenance genes, such as Nbs1, Mre11 and CtIP, in the maintenance of genome stability.
Select Publications
-
Wang, H.; Li, Y.; Truong, L. N.; Shi, L. Z.; Hwang, P. Y.; He, J.; Do, J.; Cho, M. J.; Li, H.; Negrete, A.; Shiloach, J.; Berns, M. W.; Shen, B.; Chen, L.; Wu, Xiaohua CtIP maintains stability at common fragile sites and inverted repeats by end resection-independent endonuclease activity. Molecular Cell 2014, 54, 1012-1021.
View -
Truong, L. N.; Li, Y.; Shi, L. Z.; Hwang, P. Y.; He, J.; Wang, H.; Razavian, N.; Berns, M. W.; Wu, Xiaohua Microhomology-mediated end joining and homologous recombination share the initial end resection step to repair DNA double-strand breaks in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America 2013, 110, 7720-7725.
View -
Wang, Hailong; Shi, Linda Z.; Wong, Catherine C L C.; Han, Xuemei; Hwang, Patt Y.; Truong, La N.; Zhu, Qingyuan; Shao, Zhengping; Chen, David J.; Berns, Michael W.; Yates, John R.; Chen, Longchuan; Wu, Xiaohua The interaction of CtIP and Nbs1 connects CDK and ATM to regulate HR-mediated double-strand break repair. 2013, 9, e1003277.
-
Olson, Erin; Nievera, Christian J.; Liu, Enbo; Lee, Alan Y.; Chen, Longchuan; Wu, Xiaohua The Mre11 complex mediates the S-phase checkpoint through an interaction with replication protein A. 2007, 27, 6053-67.
