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The Asahara Laboratory - Publications

Selected Publications:

  1. Yamamoto H, Uchida Y, Kurimoto R, Chiba T, Matsushima T, Ito Y, Inotsume M, Miyata K, Watanabe K, Inada M, Goshima N, Uchida T, Asahara H. RNA-binding protein LIN28A upregulates HIF1α by post-transcriptional regulation via direct binding to UGAU motifs. J Biol Chem. 2023 Jan;299(1):102791. ​ 
  2. Ono Y, Schlesinger S, Fukunaga K, yambe S, Sato T, Sasaki T, Shukunami C, Asahara H, Inui M. Scleraxis-lineage cells are required for correct muscle patterning. Development. 2023 May 15;150(10).
  3. Nakamichi R, Ma S, Nonoyama T, Chiba T, Kurimoto R, Ohzono H, Olmer M, Shukunami C, Fuku N, Wang F, Morrison E, Pitsiladis YP, Ozaki T, D’Lima D, Lotz M, Patapoutian A, Asahara H. The mechanosensitive ion channel PIEZO1 is expressed in tendons and regulates physical performance. Sci Transl Med. 2022 Jun;14(647):eabj5557. ​ 
  4. Nakamichi R, Asahara H. Motion-capture Analysis of Mice Using a Video Recorded on an iPhone Camera. Bio Protoc. 2022  12(21): e4539. 
  5. Takada K, Chiba T, Miyazaki T, Yagasaki L, Nakamichi R, Iwata T, Moriyama K, Harada H, Asahara H. Single cell RNA sequencing reveals critical functions of Mkx in periodontal ligament homeostasis. Frontiers in Cell and Developmental Biology. 2022 Feb 4;10:795441.
  6. Fujii Y, Liu L, Yagasaki L, Inotsume M, Chiba T, Asahara H. Cartilage Homeostasis and Osteoarthritis. Int J Mol Sci. 2022 Jun 5;23(11):6316.ligament homeostasis. Frontiers in Cell and Developmental Biology. 2022 Feb 4;10:795441. ​ 
  7. Murakami T, Takahata Y, Hata K, Ebina K, Hirose K, Ruengsinpinya L, Nakaminami Y, Etani Y, Kobayashi S, Maruyama T, Nakano H, Kaneko T, Toyosawa S, Asahara H, Nishimura R. Semaphorin 4D induces articular cartilage destruction and inflammation in joints by transcriptionally reprogramming chondrocytes. Sci Signal. 2022 Nov;15(758):eabl5304. 
  8. Kawata M, Teramura T, Ordoukhanian P, Head SR, Natarajan P, Sundaresan A, Olmer M, Asahara H, Lotz MK. Krüppel-like factors-4 and -2 are important regulators of joint tissue cells and protect against tissue destruction and inflammation in osteoarthritis. Annals Rheum Dis. 2022 May 9:annrheumdis-2021-221867. 
  9. Sasako T, Umehara T, Soeda K, Kaneko K, Suzuki M, Kobayashi N, Okazaki Y, Tamura-Nakano M, Chiba T, Accili D, Kahn CR, Noda T, Asahara H, Yamauchi T, Kadowaki T, Ueki K. Deletion of skeletal muscle Akt1/2 causes osteosarcopenia and reduces lifespan in mice. Nat Commun. 2022 Oct 5;13(1):5655.
  10. Tsutsumi H, Kurimoto R, Chiba T, Nakamichi R, Matsushima T, Fujii Y, Sanada R, Kato T, Shishido K, Sakamaki Y, Kimura T, Kishida A, Asahara H. Generation of a tendon-like tissue from human iPS cells. J Tissue Eng. 2022 Jan 21;13:20417314221074018. 
  11. Ito Y, Matsuzaki T, Ayabe F, Mokuda S, Kurimoto R, Matsushima T, Mochizuki Y, Inotsume M, Tsutsumi H, Liu L, Shinohara M, Tanaka Y, Nakamichi R, Nishida K, Lotz M, Asahara H. Both microRNA-455-5p and -3p repress hypoxia-inducible factor-2α expression and coordinately regulate cartilage homeostasis. Nat Commun. 2021 Jul 6;12(1):4148. ​ 
  12. Uchida Y, Matsushima T, Kurimoto R, Chiba T, Inutani Y, Asahara H. Identification of chemical compounds regulating PD-L1 by introducing HiBiT-tagged cells. FEBS Letters. 2021 Mar;595(5):563-576. 
  13. Miyazaki T, Kurimoto R, Chiba T, Matsushima T, Nakamichi R, Tsutsumi H, Takada K, Yagasaki L, Kato T, Shishido K, Kobayashi Y, Matsumoto T, Moriyama K, Asahara H. Mkx regulates the orthodontic tooth movement via osteoclast induction. 2021 May 14. ​ 
  14. Chiba T, Kurimoto R, Matsuhima T, Ito Y, Nakamichi R, Lotz M, Asahara H. MicroRNA expression profiling, and target identification and validation in chondrocytes. Methods in Molecular Biology Book. 2021;2245:151-166. 
  15. Nakamichi R, Asahara H. Regulation of tendon and ligament differentiation. 2021 Feb;143:115609.
  16. Yamamoto H, Uchida Y, Chiba T, Kurimoto R, Matsushima T, Ishikawa C, Li H, Shiga T, Muratani M, Uchida T, Asahara H. Transcriptome analysis of sevoflurane exposure effects at the different brain regions. PloS One. 2020 Dec 15;15(12):e0236771.
  17. Kurimoto R, Chiba T, Ito Y, Matsushima T, Yano Y, Miyata K, Yashiro Y, Suzuki T, Tomita K, Asahara H. The tRNA pseudouridine synthase TruB1 regulates the maturation and function of let-7 miRNA. EMBO J. 2020  e104708. 
  18. Sato T, Kataoka K, Ito Y, Yokoyama S, Inui M, Mori M, Takahashi S, Akita K, Takada S, Ueno-Kudoh H, Asahara H. Lin28a/let-7 Pathway Modulates the Hox Code via Polycomb Regulation during Axial Patterning in Vertebrates. 2020  May 29;9:e53608. ​ ​ ​ ​ ​ ​ ​ ​ ​ 
  19. Kataoka K, Kurimoto R, Tsutsumi H, Chiba T, Kato T, Shishito K, Kato M, Ito Y, Cho Y, Hoshi O, Mimata A, Sakamaki Y, Nakamichi R, Lotz MK, Naruse K, Asahara H. In vitro neo-genesis of tendon-like tissue by combination of Mohawk and a three dimensional cyclic mechanical stretch culture system. Frontiers in Cell and Developmental Biology. 2020 Jun 2;8:307. 
  20. Yano Y, Chiba T, Asahara H. Analysis of the mouse Y chromosome by single molecule sequencing with Y chromosome enrichment. Frontiers in Genetics. 2020 May 7;11:406. 
  21. Nakamchi R, Kurimoto R, Tabata Y,  Asahara H. Transcriptional, Epigenetic and microRNA regulation of growth plate. 2020 Aug;137:115434. 
  22. Gracey E, Burssens A, Cambre I, Schett G, Lories R, McInnes I, Asahara H, Elewaut D. Tendon and ligament mechanical loading in the pathogenesis of inflammatory arthritis. Nat Rev Rheumatol. 2020 Apr;16(4):193-207. 
  23. Nakamichi R, Asahara H. The transcription factors regulating intervertebral disc development. JOR Spine. 2020 Feb 20. ;3(1):e1081. Mokuda S, Nakamichi R, Ito Y, Asahara H. Genetic, Epigenetic, and MicroRNA Regulation of Osteoarthritis. 2020. In: Zaidi, M (ed.) Encyclopedia of Bone Biology, vol.[1], pp. 641-651. Oxford: Academic Press. 
  24. Lee KI, Gamini R, Olmer M, Ikuta Y, Hasei J, Baek J, Alvarez-Garcia O, Grogan SP, D'Lima DD, Asahara H, Su AI, Lotz MK. Mohawk is a meniscus transcription factor that promotes meniscus cell phenotype, tissue repair and reduces osteoarthritis severity. Sci Transl Med. 2020 Oct 28;12(567):eaan7967.
  25. Yamashita S, Kataoka K, Yamamoto H, Kato T, Hara S, Yamaguchi K, Renard-Guillet C, Katou Y, Shirahige K, Ochi H, Ogino H, Uchida T, Inui M, Takada S, Shigenobu S, Asahara H. Comparative analysis demonstrates cell type-specific conservation of SOX9 targets between mouse and chicken. Sci Rep. 2019 Aug 29;9(1):12560. 
  26. Mokuda S, Nakamichi R, Matsuzaki T, Ito Y, Sato T, Miyata K, Inui M, Olmer M, Sugiyama E, Lotz M,  Asahara H. Wwp2 maintains cartilage homeostasis through regulation of Adamts5. Nat Commun. 2019 Jun 3;10(1):2429. 
  27. Sasako T, Ohsugi M, Kubota N, Itoh S, Okazaki Y, Terai A, Kubota T, Yamashita S, Nakatsukasa K, Kamura T, Iwayama K, Tokuyama K, Kiyonari H, Furuta Y, Shibahara J, Fukayama M, Enooku K, Okushin K, Tsutsumi T, Tateishi R, Tobe K, Asahara H, Koike K, Kadowaki T, Ueki K. Hepatic Sdf2l1 controls feeding-induced ER stress and regulates metabolism. Nat Commun. 2019 Feb 27;10(1):947
  28. Mitsumura T, Ito Y, Chiba T, Matsushima T, Kurimoto R, Tanaka Y, Kato T, Uchida K, Ito T, Yamamoto K, Eishi Y, Kitagawa M, Miyazaki Y, Inase N, Asahara H. Ablation of miR-146b in mice causes hematopoietic malignancy. Blood Adv. 2018 Dec 11;2(23):3483-3491. 
  29. Mochizuki Y, Chiba T, Kataoka K, Yamashita S, Sato T, Kato T, Takahashi K, Miyamoto T, Kitazawa M, Hatta T, Natsume T, Takai S, Asahara H. Combinatorial CRISPR/Cas9-approach to elucidate a far-upstream enhancer complex for tissue-specific Sox9 expression. Dev Cell. 2018 Sep 24;46(6):794-806. 
  30. Inui M, Mokuda S, Sato T, Tamano M, Takada S, Asahara H. Dissecting the roles of miR-140 and its host gene. Nat Cell Biol. 2018 May;20(5):516-518. 
  31. Matsuzaki T, Alvarez-Garcia O, Mokuda S, Nagira K, Olmer M, Gamini R, Miyata K, Akasaki Y, Su AI, Asahara H, Lotz MK. FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis. Sci Transl Med. 2018 Feb 14;10(428).
  32. Ito Y, Inoue A, Seers T, Hato Y, Igarashi A, Toyama T, Taganov K, Boldin M, Asahara H. Identification of targets of tumor suppressor microRNA-34a using a reporter library system. Proc Natl Acad Sci U S A. 2017 Apr 11;114(15):3927-3932. 
  33. Matsuzaki T, Alvarez-Garcia O, Mokuda S, Nagira K, Olmer M, Gamini R, Miyata K, Akasaki Y, Su AI, Asahara H, Lotz MK. FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis. Sci Transl Med. 2018 Feb 14;10(428).
  34. Hasei J, Teramura T, Takehara T, Onodera Y, Horii T, Olmer M, Hatada I, Fukuda K, Ozaki T, Lotz M, Asahara H. TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes. Sci Rep. 2017 Feb 21;7:42990. 
  35. Nakasuji T, Ogonuki N, Chiba T, Kato T, Shiozawa K, Yamatoya K, Tanaka H, Kondo T, Miyado K, Miyasaka N, Kubota T, Ogura A, Asahara H. Complementary critical functions of Zfy1 and Zfy2 in mouse spermatogenesis and reproduction. PLoS Genetics. 2017 Jan 23;13(1):e1006578. 
  36. Koda N, Sato T, Shinohara M, Ichinose S, Ito Y, Nakamichi R, Kayama T, Kataoka K, Suzuki H, Moriyama K, Asahara H. Mohawk transcription factor regulates homeostasis of the periodontal ligament. 2017 Jan 15;144(2):313-320. 
  37. Naito M, Mori M, Inagawa M, Miyata K, Hashimoto N, Tanaka S, Asahara H. Dnmt3a Regulates Proliferation of Muscle Satellite Cells via p57Kip2. PLoS Genetics. 2016 Jul 14;12(7):e1006167. 
  38. Nakamichi R, Ito Y, Inui M, Onizuka N, Kayama T, Kataoka K, Suzuki H, Mori M, Inagawa M, Ichinose S, Lotz M, Sakai D, Masuda K, Ozaki T, Asahara H. Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs. Nat Commun. 2016 Aug 16;7:12503. 
  39. Suzuki H, Ito Y, Shinohara M, Yamashita S, Ichinose S, Kishida A, Oyaizu T, Kayama T, Nakamichi R, Koda N, Yagishita K, Lotz M, Okawa A, Asahara H. Gene targeting of the transcription factor Mohawk in rats causes heterotopic ossification of Achilles tendon via failed tenogenesis. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7840-5. 
  40. Kayama T, Mori M, Ito Y, Matsushima T, Nakamichi R, Suzuki H, Ichinose S, Saito M, Marumo K, Asahara H. Gtf2ird1-Dependent Mohawk Expression Regulates Mechanosensing Properties of the Tendon. Mol Cell Biol. 2016 Mar 31;36(8):1297-309. ​ 
  41. Matsubara Y, Kato T, Kashimada K, Tanaka H, Zhi Z, Ichinose S, Mizutani S, Morio T, Chiba T, Ito Y, Saga Y, Takada S, Asahara H. TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis. Stem Cells Dev. 2015 May15;24(10):1164-70. 
  42. Miyata K, Miyata T, Nakabayashi K, Okamura K, Naito M, Kawai T, Takada S, Kato K, Miyamoto S, Hata K, Asahara H. DNA methylation analysis of human myoblasts during in vitro myogenic differentiation: de novo methylation of promoters of muscle-related genes and its involvement in transcriptional down-regulation. Hum Mol Genet. 2015 Jan 15;24(2):410-23. ​ 
  43. Watanabe T, Oyama T, Asada M, Harada D, Ito Y, Inagawa M, Suzuki Y, Sugano S, Katsube K, Karsenty G, Komori T, Kitagawa M, Asahara H. MAML1 enhances the transcriptional activity of Runx2 and plays a role in bone development. PLoS Genetics. 2013;9(1):e1003132.
  44. Yamashita S, Miyaki S, Kato Y, Yokoyama S, Sato T, Barrionuevo F, Akiyama H, Scherer G, Takada S, Asahara H. L-Sox5 and Sox6 proteins enhance chondrogenic miR-140 microRNA expression by strengthening dimeric Sox9 activity. J Biol Chem. 2012 Jun 22;287(26):22206-15. 
  45. Uchibe K, Shimizu H, Yokoyama S, Kuboki T, Asahara H. Identification of novel transcription-regulating genes expressed during murine molar development. Dev Dyn. 2012 Jul;241(7):1217-26.
  46. Asada M, Rauch A, Shimizu H, Maruyama H, Miyaki S, Shibamori M, Kawasome H, Ishiyama H, Tuckermann J, Asahara H. DNA binding-dependent glucocorticoid receptor activity promotes adipogenesis via Krüppel-like factor 15 gene expression. Lab Invest. 2011 Feb;91(2):203-15.
  47. Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, Kato Y, Takemoto F, Nakasa T, Yamashita S, Takada S, Lotz MK, Ueno-Kudo H, Asahara H. MicroRNA-140 plays dual roles in both cartilage development and homeostasis. Genes Dev. 2010 Jun 1;24(11):1173-85 
  48. Ito Y, Toriuchi N, Yoshitaka T, Ueno-Kudoh H, Sato T, Yokoyama S, Nishida K, Akimoto T, Takahashi M, Miyaki S, Asahara H. The Mohawk homeobox gene is a critical regulator of tendon differentiation. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10538-42 
  49. Higashiyama R, Miyaki S, Yamashita S, Yoshitaka T, Lindman G, Ito Y, Sasho T, Takahashi K, Lotz M, Asahara H. Correlation between MMP-13 and HDAC7 expression in human knee osteoarthritis. Mod Rheumatol. 2010 Feb;20(1):11-7.
  50. Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M,  Asahara H. A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. Dev Cell. 2009 Dec;17(6):836-48.