Louis Scampavia, PhD

Scientific Director, Sr
Department of Molecular Medicine
Florida Campus


 Email

Research Focus

Progress in drug discovery is often coupled to parallel advancements in instrument technology. In our department, High Throughput Screening (HTS) robotics is employed to accelerate the drug discovery process thorough full automation of large scale screening experiments. HTS technology can be used to execute and analyze hundreds of thousand of experiments against large compound libraries to identify a few select compounds of therapeutic value. These "therapeutic leads" provide important insight and basis for the medicinal development of novel drugs for clinical application.

 Research efforts are currently focused on:

  • Working with collaborators to develop biological assays for HTS compatibility.
  • Performing exploratory HTS campaigns for drug lead discovery.
  • Research and development of novel instruments and technologies for the advancement of HTS robotics.

Collaborative efforts are coordinated with a large community of scientists and include TSRI faculty, Florida researchers, and scientists funded through the National Institutes of Health sponsored Molecular Library Screening Center Network program (http://nihroadmap.nih.gov/).


Education

Ph.D. (Chemistry), 1996
B.S. (Chemistry), 1991
B.S. (Genetics), University of California, Berkeley, 1979

Professional Experience

2007-2017 Associate Professor (Joint Appointment), Molecular Therapeutics, Scripps Research
2001-2005 CEO, SiChrom Inc.
2001-2005 Principal Investigator, SiChrom Inc.

Selected References

All Publications

Wang C, Diez J, Park H, Spicer TP, Scampavia L, Becker-Pauly C, Fields G, Minond D, Bannister T, Discovery and Optimization of Selective Inhibitors of Meprin a (Part II) arrow Pharmaceuticals.  2021 February.

Hou S, Diez J, Wang C, Becker-Pauly C, Fields G, Bannister T, Spicer TP, Scampavia L, Minond D, Discovery and Optimization of Selective Inhibitors of Meprin a (Part I) arrow Pharmaceuticals.  2021 January.

Wang L, Cheng CM, Qin J, Xu M, Kao CY, Shi J, You E, Gong W, Rosa LP, Chase P, Scampavia L, Madoux F, Spicer T, Hodder P, Xu HE, Tsai SY, Tsai MJ. Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment. Sci Adv. 2020 May;6(18):eaaz8031. doi: 10.1126/sciadv.aaz8031. eCollection 2020 May. PubMed PMID: 32494682; PubMed Central PMCID: PMC7190335.

Pan CH, Otsuka Y, Sridharan B, Woo M, Leiton CV, Babu S, Torrente Gonçalves M, Kawalerski RR, K Bai JD, Chang DK, Biankin AV, Scampavia L, Spicer T, Escobar-Hoyos LF, Shroyer KR. An unbiased high-throughput drug screen reveals a potential therapeutic vulnerability in the most lethal molecular subtype of pancreatic cancer. Mol Oncol. 2020 Aug;14(8):1800-1816. doi: 10.1002/1878-0261.12743. Epub 2020 Jul 4. PubMed PMID: 32533886; PubMed Central PMCID: PMC7400780

Grandjean JMD, Madhavan A, Cech L, Seguinot BO, Paxman RJ, Smith E, Scampavia L, Powers ET, Cooley CB, Plate L, Spicer TP, Kelly JW, Wiseman RL. Pharmacologic IRE1/XBP1s activation confers targeted ER proteostasis reprogramming. Nat Chem Biol. 2020 Oct;16(10):1052-1061. doi: 10.1038/s41589-020-0584-z. Epub 2020 Jul 20. PubMed PMID: 32690944; PubMed Central PMCID: PMC7502540.

Llamosas N, Arora V, Vij R, Kilinc M, Bijoch L, Rojas C, Reich A, Sridharan B, Willems E, Piper DR, Scampavia L, Spicer TP, Miller CA, Holder JL, Rumbaugh G. SYNGAP1 Controls the Maturation of Dendrites, Synaptic Function, and Network Activity in Developing Human Neurons. J Neurosci. 2020 Oct 7;40(41):7980-7994. doi: 10.1523/JNEUROSCI.1367-20.2020. Epub 2020 Sep 4. PubMed PMID: 32887745; PubMed Central PMCID: PMC7548701.

Smith E, Davis-Gardner ME, Garcia-Ordonez RD, Nguyen TT, Hull M, Chen E, Baillargeon P, Scampavia L, Strutzenberg T, Griffin PR, Farzan M, Spicer TP. High-Throughput Screening for Drugs That Inhibit Papain-Like Protease in SARS-CoV-2. SLAS Discov. 2020 Dec;25(10):1152-1161. doi: 10.1177/2472555220963667. Epub 2020 Oct 10. PubMed PMID: 33043784; PubMed Central PMCID: PMC7550789.

Otsuka Y, Airola MV, Choi YM, Coant N, Snider J, Cariello C, Saied EM, Arenz C, Bannister T, Rahaim R Jr, Hannun YA, Shumate J, Scampavia L, Haley JD, Spicer TP. Identification of Small-Molecule Inhibitors of Neutral Ceramidase (nCDase) via Target-Based High-Throughput Screening. SLAS Discov. 2021 Jan;26(1):113-121. doi: 10.1177/2472555220945283. Epub 2020 Jul 31. PubMed PMID: 32734807; PubMed Central PMCID: PMC7749003.

Smith E, Dukovski D, Shumate J, Scampavia L, Miller JP, Spicer TP. Identification of Compounds That Promote Readthrough of Premature Termination Codons in the CFTR. SLAS Discov. 2021 Feb;26(2):205-215. doi: 10.1177/2472555220962001. Epub 2020 Oct 5. PubMed PMID: 33016182; PubMed Central PMCID: PMC7838340.

Thenin-Houssier S, de Vera IM, Pedro-Rosa L, Brady A, Richard A, Konnick B,
Opp S, Buffone C, Fuhrmann J, Kota S, Billack B, Pietka-Ottlik M, Tellinghuisen
T, Choe H, Spicer T, Scampavia L, Diaz-Griffero F, Kojetin DJ, Valente ST.
Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication. Antimicrob
Agents Chemother. 2016 Mar 25;60(4):2195-208. doi: 10.1128/AAC.02574-15. Print
2016 Apr. PubMed PMID: 26810656; PubMed Central PMCID: PMC4808204.

Wang J, Fang P, Chase P, Tshori S, Razin E, Spicer TP, Scampavia L, Hodder P, Guo
M. Development of an HTS-Compatible Assay for Discovery of Melanoma-Related
Microphthalmia Transcription Factor Disruptors Using AlphaScreen Technology. SLAS
discovery. 2017; 22(1):58-66. PubMed [journal] PMID: 27827304

Spicer TP. Drug Discovery Targeting COVID-19. SLAS Discov. 2020 Dec;25(10):1095-1096. doi: 10.1177/2472555220970966. PubMed PMID: 33215959.

Hou S, Madoux F, Scampavia L, Janovick JA, Conn PM, Spicer TP. Drug Library
Screening for the Identification of Ionophores That Correct the Mistrafficking
Disorder Associated with Oxalosis Kidney Disease. SLAS discovery. 2017;
:2472555217689992. PubMed [journal] PMID: 28346094

Madoux F, Dreymuller D, Pettiloud JP, Santos R, Becker-Pauly C, Ludwig A, Fields
GB, Bannister T, Spicer TP, Cudic M, Scampavia LD, Minond D. Discovery of an
enzyme and substrate selective inhibitor of ADAM10 using an exosite-binding
glycosylated substrate. Scientific reports. 2016; 6(1):11. PubMed [journal] PMID:
27920432

Janovick JA, Spicer TP, Smith E, Bannister TD, Kenakin T, Scampavia L, Conn PM.
Receptor antagonism/agonism can be uncoupled from pharmacoperone activity.
Molecular and cellular endocrinology. 2016; 434:176-85. NIHMSID: NIHMS801618
PubMed [journal] PMID: 27389877, PMCID: PMC4983495

Jambrina E, Cerne R, Smith E, Scampavia L, Cuadrado M, Findlay J, Krambis MJ,
Wakulchik M, Chase P, Brunavs M, Burris KD, Gallagher P, Spicer TP, Ursu D. An
Integrated Approach for Screening and Identification of Positive Allosteric
Modulators of N-Methyl-D-Aspartate Receptors. Journal of biomolecular screening.
2016; 21(5):468-79. PubMed [journal] PMID: 26838761

Plate L, Cooley CB, Chen JJ, Paxman RJ, Gallagher CM, Madoux F, Genereux JC,
Dobbs W, Garza D, Spicer TP, Scampavia L, Brown SJ, Rosen H, Powers ET, Walter P,
Hodder P, Wiseman RL, Kelly JW. Small molecule proteostasis regulators that
reprogram the ER to reduce extracellular protein aggregation. eLife. 2016; 5.
PubMed [journal] PMID: 27435961, PMCID: PMC4954754

Ewing H, Fernández-Vega V, Spicer TP, Chase P, Brown S, Scampavia L, Roush WR,
Riley S, Rosen H, Hodder P, Lambeau G, Gelb MH. Fluorometric High-Throughput
Screening Assay for Secreted Phospholipases A2 Using Phospholipid Vesicles.
Journal of biomolecular screening. 2016; 21(7):713-21. PubMed [journal] PMID:
27146384

Smith E, Janovick JA, Bannister TD, Shumate J, Scampavia L, Conn PM, Spicer TP.
Identification of Potential Pharmacoperones Capable of Rescuing the Functionality
of Misfolded Vasopressin 2 Receptor Involved in Nephrogenic Diabetes Insipidus.
Journal of biomolecular screening. 2016; 21(8):824-31. PubMed [journal] PMID:
27280550

Janovick JA, Spicer TP, Bannister TD, Scampavia L, Conn PM. Pharmacoperone
rescue of vasopressin 2 receptor mutants reveals unexpected constitutive activity
and coupling bias. PLoS One. 2017 Aug 2;12(8):e0181830. doi:
10.1371/journal.pone.0181830. eCollection 2017. PubMed PMID: 28767678.

Madoux F, Tanner A, Vessels M, Willetts L, Hou S, Scampavia L, Spicer TP. A 1536-Well 3D Viability Assay to Assess the Cytotoxic Effect of Drugs on Spheroids. SLAS discovery. 2017; :2472555216686308. PubMed [journal] PMID: 28346088

Smith E, Janovick JA, Bannister TD, Shumate J, Ganapathy V, Scampavia L, Spicer TP. Rescue of mutant gonadotropin-releasing hormone receptor function independent of cognate receptor activity. Sci Rep. 2020 Jun 29;10(1):10579. doi: 10.1038/s41598-020-67473-w. PubMed PMID: 32601341; PubMed Central PMCID: PMC7324376.


Links

Scripps Florida Scientists Discover New Compounds with Potential to Treat Persistent Tuberculosis

Making Plans: Scripps Florida Screening Center Expands Its Collaborations

Using bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates