Directory | Contact | Careers | Intranet | Home

Scripps Research Joint Appointments

Professor, Department of Molecular and Experimental Medicine
Professor, Department of Chemical Physiology
Faculty, Kellogg School of Science and Technology

Research Focus

Carbohydrate Binding Proteins in Regulation Of Immune Function

Carbohydrate binding proteins are increasingly recognized to mediate key aspects of cell trafficking and cell signaling in the immune system. At least three families of carbohydrate binding proteins are known to mediate cellular communication among leukocytes. The siglec family now has thirteen members which are expressed on the surface of various white blood cells (e.g. B cells, NK cells, eosinophils, monocytes etc.), and all recognize sialic acid (NeuAc) containing carbohydrate ligands. The best understood for its function is CD22 (siglec-2), which is known to be a negative regulator of B cell receptor signaling. The carbohydrate ligand of CD22 is the sequence  NeuAca2,6Gal on N-linked oligosaccharides of glycoproteins. It is required for normal CD22 function since its absence in mutant mice results in marked immuno-suppression in response to vaccination. Our goal is to elucidate the roles of siglec receptors in immune function, and to define biochemical basis for how the interaction with their carbohydrate ligands modulate their function.

Education

Ph.D., Biochemistry, University of Illinois at Urbana-Champaign , 1974
M.S., Biochemistry, University of Illinois at Urbana-Champaign , 1971
A.B., Chemistry/Biology, MacMurray College, 1970

Professional Experience

Dept. of Biochemistry, Duke University, Postdoctoral Fellow 1974-1978; Dept. of Biol. Chem., Univ. California Los Angeles, Assistant Professor 1978-1981, Associate Professor 1981-1985, Professor and Vice Chair 1985-1990; Cytel Corporation, V.P. Research Development and Board of Directors 1990-1996, Chief Scientific Officer and General Manager and Board of Directors 1996-1999; Professor, Dept. of Chemical Physiology, The Scripps Research Institute, 1999-present.

Awards & Professional Activities

Chair-elect, ACS Division of Carbohydrate Chemistry (2012-present); National Academy of Sciences Glycoscience Committee (2011-2012); Principle Investigator, Consortium Functional Glycomics, http://www.functionalglycomics.org (2001-2011); Co-chair Human Glycomics/Proteomics Initiative (HGPI), http://www.hupo.org/research/hgpi/ (2005-present); President, The Society for Glycobiology (2002-2003); Editorial board, Glycobiology (1990-present); Scientific Advisory Boards, Neose Technologies Inc (1999-2008), Virdante Pharmaceuticals Inc. (2007-present), Zacharon (2006-2009), Nexbio (2004-present), Alberta Ingenuity Center for Carbohydrate Science-AICCS, (2003-present), Institute for Biological Sciences-IBS, NRC, Ottawa (2004-present), ; Awards: Barnett Lecture, 2008; Bijvoet Medal, 2008; Karl Meyer Award, 2009

.

Selected References

A complete list of publications can be viewed at http://www.scripps.edu/chemphys/paulson/publications.html

Also available listing at Google Scholar

 

Rillahan, C.D., Antonopoulos, A., Lefort, C.T., Sonon, R., Azadi, A., Ley, K., Dell, A., Haslam, S.M., and Paulson, J.C. (2012) Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome. Nat. Chem. Biol. 8:661-668.  

Rillahan, C.D., Schwartz, E., McBride, R., Fokin, V.V., and Paulson, J.C. (2012) Click and Pick: Identification of Sialoside Analogues for Siglec-Based Cell Targeting. Angew Chem Int Ed Engl. 51(44):11014-18  

Nycholat, C.M., McBride, R., Ekiert, D.C., Xu, R., Rangarajan, J., Peng, W., Razi, N., Gilbert, M., Wakarchuk, W., Wilson, I.A., and Paulson, J.C. (2012) Recognition of Sialylated Poly-N-acetyllactosamine Chains on N- and O-Linked Glycans by Human and Avian Influenza A Virus Hemagglutinins. Angew Chem Int Ed Engl. 51(20):4860-63

Paulson, J.C., Macauley, M.S., and Kawasaki, N. (2012) Siglecs as sensors of self in innate and adaptive immune responses. Ann N Y Acad Sci. 1253(1):37-48. 

Chen, W.C., Completo, G.C., Sigal, D.S., Crocker, P.R., Saven, A. and Paulson, J.C. (2010) In vivo targeting of B-cell lymphoma with glycan ligands of CD22. Blood. 115(23):4778-86. 

Duong, B.H., Tian, H., Ota, T., Completo, G., Han, S., Vela, J.L., Ota, M., Kubitz, M., Bovin, N., Paulson, J.C., and Nemazee, D. (2010) Decoration of T-independent antigen with ligands for CD22 and Siglec-G can suppress immunity and induce B cell tolerance in vivo. J Exp Med. 207(1):173-187.

Links

After the Genome, Part 2 : The Study of the Glycome

Life, Sugar-Coded