Faculty, Graduate Program
The goal of our research is to understand molecular mechanisms of redox-based regulation of protein function and the regulation by redox systems of complex physiological processes. To achieve these goals, we develop and apply new technologies that cut across the traditional boundaries of chemical and biological sciences.
Chemical Tools to Map Redox Signaling Pathways
Oxidative thiol modifications have emerged as a central mechanism for dynamic post-translational regulation of all major protein classes and correlate with many disease states. We focus on the development and application of chemoselective probes as tools to profile these modifications in cell-based systems and to identify biomarkers of oxidative stress.
New methods for comprehensive and quantitative analysis of the "thiol" proteome are being developed and applied to the discovery of cellular signaling networks and regulatory mechanisms that involve oxidation of protein cysteine residues.
Redox-Dependent Kinase and Phosphatase Inhibitors
Our lab is taking a rational design approach toward the development irreversible, small-molecule inhibitors that target the oxidized forms of functionally important cysteine residues in kinases and phosphatases.
Microbial Sulfur Metabolism
Projects in this area focus on pathways of sulfur metabolism in human pathogens, such as Mycobacterium tuberculosis and the study of enzymes that are critical for virulence and survival using a range of chemical, biochemical, structural, and computational techniques.
B.A., Chemistry, Mills College, 1996
Ph.D., Chemistry, Stanford University, 2003
2003 Damon Runyon Postdoctoral Fellowship 2006 Leukemia and Lymphoma Society Special Fellow Award 2008 American Heart Association Scientist Development Award 2010 Camille Dreyfus Teacher-Scholar Award
Depuydt, M.; Leonard, S. E.; Vertommen, D.; Denoncin, K.; Morsomme, P.; Wahni, K.; Messens, J.; Carroll, K. S.; Collet, J. F., A periplasmic reducing system protects single cysteine residues from oxidation. Science 2009, 326 (5956), 1109-11.
Seo, Y. H.; Carroll, K. S., Profiling protein thiol oxidation in tumor cells using sulfenic acid-specific antibodies. Proc Natl Acad Sci U S A 2009, 106 (38), 16163-8.
Leonard, S. E.; Reddie, K. G.; Carroll, K. S., Mining the thiol proteome for sulfenic acid modifications reveals new targets for oxidation in cells. ACS Chem Biol 2009, 4 (9), 783-99.
Paulsen, C. E.; Carroll, K. S., Chemical dissection of an essential redox switch in yeast. Chem Biol 2009, 16 (2), 217-25.