Scientific Report 2007
Weissmann, M.D., Ph.D.
Lasmézas, D.V.M., Ph.D.
Senior Staff Scientist
Nicole Salès, Ph.D.
Sukhvir Mahal, Ph.D.
Senior Research Associates
Chris Baker, Ph.D.
Carlos Coito, Ph.D.
Shawn Browning, Ph.D.
Altan Ercan, Ph.D.
Natalia Fernandez-Borges, Ph.D.
Yervand Karapetyan, M.D.
Jiali Li, Ph.D.
Paula Saá Prieto, Ph.D.
Prem Subramaniam, Ph.D.
Minghai Zhou, Ph.D.
prion protein (green) is expressed at the surface and inside cultured neurons, near
the nucleus of the cell (blue) but also, interestingly, at the extremity of the
neurite. The latter is best seen on the large central cell but also on smaller bipolar
cells. This observation suggests that the normal prion protein could be involved
in synaptic transmission. In prion diseases, the prion protein is converted into
a misfolded form and causes the death of the neurons. These cultured neuronal cells
are not infected, but can be infected, with prions. The cells were labeled with
DAPI for the nuclei and with a specific antibody coupled to Alexa 488. They were
then examined under objective x20 with a Zeiss microscope, and the images were analyzed
by using Zeiss AxioView software. Work done by Nicole Sales, Ph.D., senior staff
scientist, in the laboratory of Corinne Lasmézas, D.V.M., Ph.D., professor.
Scripps Florida Reports
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