Faculty, Graduate Program
The Carroll lab has an proven track record of attacking fundamental problems in redox biology through a powerful, interdisciplinary approach that integrates synthetic chemistry with proteomics, biochemistry, and cell biology.
An overarching goal of our research program is to understand the biological chemistry and molecular mechanisms of redox-based cellular regulation and signal transduction, with particular emphasis on the role of cysteine oxidation, a ubiquitous and conserved mechanism for controlling protein function. We are also exploring the therapeutic potential of redox-regulated protein function by developing an entirely new class of inhibitors that targets oxidized cysteine residues of key proteins involved in human disease, such as kinases and phosphatases. We also investigate sulfur metabolic pathways that are essential for infection and long-term survival of human pathogens, such as Mycobacterium tuberculosis and leverage novel discoveries to develop new antimicrobial therapies.
Ultimately, our goal is to accelerate the discovery of key regulatory nodes of redox-signaling networks, profile changes in protein cysteine oxidation associated with disease, and harness this information for the development of new diagnostic and therapeutic approaches.
Students in the lab receive broad-based training in experimental techniques ranging from synthetic chemistry and mass spectrometry to cellular and in vivo animal studies. Representative skill sets and expertise in the group are given below, and students are encouraged to take multiple apporaches to ask and answer new scientific questions.
Chemical tool development: Synthetic chemistry with analytical characterization
Cell culture: Mammalian cell lines, bacteria, and primary cultures
Proteomics: Solid-phase capture, fractionation, LC-MS/MS, bioinformatics
Molecular imaging: Confocal microscopy and flow cytometry
Gene discovery: Activity-based protein profiling
Animal studies: Mouse physiology
Molecular biology: Cloning, transfections, RNAi, PCR, and CRISPR
In vitro biochemistry: Protein preparation, purification, and protein engineering
Recent research activities are summarized on our website. A complete list of our publications is located here.
B.A., Chemistry, Mills College, 1996
Ph.D., Chemistry, Stanford University, 2003
Prof. Carroll received her B.A. degree in Biochemistry from Mills College in 1996 and Ph.D. in Biochemistry from Stanford University in 2003. Her postdoctoral work was completed at the University of California, Berkeley, where she was a Damon Runyon-Walter Winchell Chancer Fund Fellow with Prof. Carolyn Bertozzi.
Prof. Carroll currently serves on the editorial board of Cell Chemical Biology, Chemical Probes.org, Molecular Biosystems, the Journal of Biology Chemistry, and is a contributing member to the ‘Faculty of 1000’. She is also the recipient of the ACS Pfizer Award in Enzyme Chemistry (2013), Camille Dreyfus Teacher-Scholar Award (2010), Scientist Development Award from American Heart Association (2008), and Special Fellow Award from the Leukemia and Lymphoma Society (2006).
Molecular basis for redox activation of epidermal growth factor receptor kinase. Truong, T. H.; Ung, P. M.; Paulsen, C. E.; Prakash, P. B.; Schlessinger, A.; Carroll, K. S. Cell Chem. Biol. 2016, 23(7): 837-48, PMC4958504.
Reactivity, selectivity, and stability in sulfenic acid detection: A comparative study of nucleophilic and electrophilic probes. Gupta, V.; Carroll, K. S. Bioconj. Chem. 2016, 27(5): 1411-8, PMC4886738.
First-in-class inhibitors of sulfur metabolism with bactericidal activity against non-replicating M. tuberculosis. Palde, P. B.; Bhaskar, A.; Pedró Rosa, L. E.; Madoux, F.; Chase, P. Gupta, V.; Spicer, T.; Scampavia, L.; Singh, A.; Carroll, K. S. ACS Chem. Biol. 2016, 11(1): 172-84, PMC4729198. *Highlighted in Nat. Chem. Biol. 2016.*