Training Grant Recipients


 

TSRI Current Trainees (2016-2019)

 
 

Robin Willenbring, Ph.D. (06/01/2018 - Present)       Mentor: Linda Sherman, Ph.D.

Defining the role of the proautoimmune risk allele, PTPN22 1858 C>T, in controlling and regulating chronic viral infection and subsequent T cell exhaustion

To elucidate the role of PTPN22 during chronic viral infection and T cell exhaustion, we will employ the well-established model of persistent viral infection, LCMV-cl13, in our aforementioned mice. Following infection, we will determine disease progression (weight and survival), viral load in serum and target organs at acute and chronic time points, and circulating and target organ anti-viral immune response (cytokine, innate cell, and adaptive cell). To determine T cell exhaustion, we will quantify the expression of inhibitory receptors on both circulating and organ specific T cells during chronic infection with LCMV-cl13. Additionally, we will isolate T cells and employ re-stimulation assays to assess cytokine production and proliferation. Next, through a series of adoptive transfer experiments, we will define which R619W-wielding immune cells are necessary and/or sufficient for proper viral control and T cell exhaustion. Completion of this study will detail the role of PTPN22 during anti-viral immune response and T cell exhaustion, providing critical groundwork for future studies defining pathogenesis associated with this gene.

 

Kip Hermann, Ph.D (12/1/16 - Present)                  Mentor: Bruce Torbett, Ph.D.

Identification of human hematopoietic stem cell lentiviral vector restriction pathways in order to improve the efficiency of lentiviral-mediated gene transfer to hematopoietic stem cells 

Inefficient gene transfer of hematopoietic stem cells (HSCs) by lentivirus has hindered the clinical application of many therapies.  We are investigating various small molecules that have been found to have an effect on lentiviral transduction of HSCs and the underlying mechanism of these effects.  Our goal is to improve lentiviral transduction of HSCs by better understanding and manipulating the pathways revealed from these small molecule studies.

 

Jacob Milligan, Ph.D. (6/26/17 - Present)               Mentor: Erica Saphire, Ph.D.

Structural studies of ebolavirus glycoproteins and cross-reactive antibodies directed against them

Viruses of the genus Ebolavirus can cause severe and often fatal hemorrhagic fever in humans and other mammals.  Of the five ebolaviruses, three are known to cause lethal infections in humans: Ebola virus, Sudan virus, and Bundibugyo virus.  There are currently no approved treatments or vaccines for any of the ebolaviruses, though several candidates are currently in clinical trials.  However, the most promising vaccine candidate is specific to only the Ebola virus and offers no protection against Sugan virus and Bundibugyo virus.  Thus, the need for a pan-ebolavirus vaccine remains unmet.  In recent years, several antibodies have been described that offer protection against all three of these viruses, though the structural basis for their cross-reactivity remains largely unknown.  The goal of my project is to determine high-resolution X-ray crystal structures and/or cryo-EM structures of ebolavirus glycoproteins in complex with these cross-reactive antibodies in order to facilitate structure-based antigen design and optimization for use in pan-ebolavirus vaccines.

 

Jordan Woehl, Ph.D. (6/20/17 - Present)                 Mentor: Dennis Wolan, Ph.D.

Identification, quantitation, and biochemical analysis of proteases secreted into the distal gut targeted for inhibition by host anti-proteolytic proteins

Through an optimized liquid chromatography tandem mass spectrometry (LC-MS/MS) metaproteomics approach on an adoptive T cell transfer murine model of chronic colitis, we have supported decades-old research that identified an increase in general proteolytic activity with fecal extracts of colitic mice and humans.  Moreover, we observed a correlated increase in host anti-proteolytic proteins (APPs) that we hypothesize are secreted into the gut lumen to quench aberrant proteolytic activity.  The exact molecular mechanism(s) causing inflammation in the gut remain poorly understood, so there is an urgent need to establish which host and microbial proteases are targeted by host APPs and if these same proteases, a different subset of proteases, or a combination of both, promote colitis.  We will elucidate the host and/or microbiome protein targets of six APPs previously identifiied among the most abundantly produced host proteins in response to distal gut inflammation and are known to collectively target proteases from the serine, cystine, and metallo subfamilies.  Ultimately, we hope to address the biological mechanism(s) of how altered proteolytic activity promotes host inflammation and assess the therapeutic potential of selective protease inhibition on inflammatory bowel diseases.

 
  Former Trainees and Current Positions (1999-2019)  
 

Steffen Bernard, Ph.D. (Wilson Lab) - Sr. Research Associate, Celgene Corp., San Diego

Cari Kessing, Ph.D. (Valente Lab) - Research Scientist, GlaxoSmithKline

Nina Timberlake, Ph.D. (Torbett Lab) - Research Scientist, Poseida Therapeutics, San Diego

Brett Marro, Ph.D. (Oldstone lab) - Research Associate, Scripps, San Diego

Robert Kirchdoefer, Ph.D. (Saphire Lab) - Research Associate, Scripps, San Diego

David Guimond, Ph.D. (Mowen Lab) - Scientist, Receptors/Celgene, San Diego

Brian Adair, Ph.D. (Yeager Lab) – Instructor, Medicine, Massachusetts General Hospital

Michael Baksh, Ph.D. (Finn Lab) - Research Scientist, Georgia Institute of Technology

Jason Botten, Ph.D. (Buchmeier Lab) – Assistant Professor, Univ. of Vermont

Amy Brideau-Andersen, Ph.D. (Chisari Lab) – Staff Scientist I, Maxygen

Emily Burke, Ph.D. (Buchmeier Lab) – Graduate Advisor UCSD Career Services Center

Ryan Burnett, Ph.D. (Gottesfeld Lab) – Research Scientist, Tocagen, Inc.

Althea Capul, Ph.D. (de la Torre Lab) – Associate Biosafety Officer, NIH

Max Chang, Ph.D. (Torbett Lab) – Programmer Analyst, UC San Diego

Jill Chrencik, Ph.D. (Kuhn Lab) – Protein Crystallographer and Biochemist, Merck

Barney Collins, Ph.D. (Wilson Lab) –  Scientist, Plex Pharmaceuticals

Jodi Connolly, Ph.D. (Nemerow Lab) – Patent Agent, Klarquist Sparkman, LLP

Christopher Cornell, Ph.D. (Whitton Lab) – Scientist, Allergan

Cromwell Cornillez-Ty, Ph.D. (Kuhn Lab) – Investment Advisor Rep., STEM Financial LLC, San Diego

Amy Cullinan, Ph.D. (Manchester Lab) – Scientist, Invitrogen

Shrimati Datta, Ph.D. (Sarvetnick Lab) – Project Coordinator, CDPH, San Francisco

Jose Garcia, Ph.Dh (Gallay Lab– Research Scientist II, Roka Bioscience

Megan Guelker, Ph.Dh (Johnson Lab)– Sr. Product Manager, Abott

Michael Giffin, Ph.D. (Torbett Lab) - Scientist, Amgen, Inc. Thousand Oaks, CA

Jennifer Head, Ph.D. (J. de la Torre Lab) – Research Scientist, AutoGenomics Inc.

Yang Hong, Ph.D. (Elder Lab) - Scientist, BioVision, Inc.

Ashley Horton, Ph.D. (Schneemann Lab) – Pharmacogenetics Scientiest, Millennium Laboratories, San Diego

Christopher Kimberlin, Ph.D. (Ollmann-Saphire Lab) – Senior Scientist, Pfizer

Kevin Koehntop, Ph.D. (Yeager Lab) – Unknown

Claire Levy, Ph.D. (Vogt Lab) –  Staff Research Associate, UC San Francisco

Crystal Moyer, Ph.D. (Newerow Lab) – Research Scientist, Mapp Biopharmaceutical

Samia Naccache, Ph.D. (Nemerow Lab) - Technical Microbiology Director, LabCorp Diagnostic Laboratories

Amy Odegard, Ph.D. (Johnson Lab) – Assistant Professor, University of Puget Sound, Tacoma, WA

Bruno Sainz, Jr., Ph.D. (Chisari Lab) – Staff Scientist, Autonoma Univesity of Madrid, Spain

Theresa Sample, Ph.D. (Stout Lab– Patent Agent, Ionis Pharmaceuticals

John Teijaro, Ph.D. (Oldstone Lab) - Asst. Professor, IMS Dept, TSRI

Joie Bernard-Trifilo, Ph.D. (Oldstone Lab) – Regional Medical Liaison, Amgen

Susan Uprichard, Ph.D. (Chisari Lab) – Assistant Professor, University of Illinois

Shuzo Urata, Ph.D. (de la Torre Lab) - Assistant Professor, Nagasaki University, Japan

Christopher Wiethoff, Ph.D. (Nemerow Lab) – Assistant Professor, Loyola University

Eugene Wu, Ph.D. (Johnson Lab) – Research Associate, Duke University

Priscilla Yang, Ph.D. (Chisari Lab) – Assistant Professor, Harvard Medical School

Jinyun Yuan, Ph.D. (Torbett Lab) – Assist. Research Professor, Saint Louis University