Paulson Laboratory

Corwin Nycholat, Ph.D.
 
The Scripps Research Institute
10550 North Torrey Pines Road, MB-209
La Jolla, CA 92037
858-784-7539(phone)
858-784-9690 (FAX)
nycholat@scripps.edu

EDUCATION:

June 2008 - Ph. D. Organic Chemistry, University of Alberta, Edmonton, AB (Canada)

2001 - M.Sc. University of Saskatchewan, Saskatoon, SK (Canada)

1997 - BSA. University of Saskatchewan, Saskatoon, SK (Canada)



PROFESSIONAL EXPERIENCES:

September 2017 to Present - Senior Staff Scientist, The Scripps Research Institute, La Jolla, CA, USA (Advisor: Prof. James C. Paulson)

July 2011 to May 2018 - Director for Carbohydrate Synthesis Core (Programs of Excellence in Glycosciences), The Scripps Research Institute, La Jolla, CA, USA

July 2014 to 2017 - Staff Scientist, The Scripps Research Institute, La Jolla, CA, USA (Advisor: Prof. James C. Paulson)

July 2011 to 2014 - Senior Research Associate, The Scripps Research Institute, La Jolla, CA, USA (Advisor: Prof. James C. Paulson)

Jan 2008 to 2011 - Postdoctoral Fellow, The Scripps Research Institute, La Jolla, CA, USA (Advisor: Prof. James C. Paulson)

2002 to 2008 - Doctoral Candidate, University of Alberta, Canada (Advisor: Prof. David R. Bundle)

2001 to 2007 - Teaching Assistant, University of Alberta, Canada

2002 - Research Assistant, University of Alberta, Canada

2001 - Research Assistant, University of Saskatchewan, Canada

1998 to 2001 - M. Sc., University of Saskatchewan, Canada (Advisor: Prof. Soledade Pedras)

1998 to 2000 - Teaching Assistant, University of Saskatchewan, Canada

1997 - Research Technician, Agriculture and Agri-Food Canada (Supervisor: Dr. Wes Taylor)

1996 to 1997 - Undergraduate Research Assistant, University of Saskatchewan, Canada (Supervisor: Prof. Malcolm Devine)



ACADEMIC AND PROFESSIONAL HONORS:

2005-2006: University of Alberta Graduate Students Association Teaching Award

2004-2005: University of Alberta Teaching Services Graduate Student Teaching Award.

2004 & 2005: Judge for Edmonton Regional Science Fairs.

2002-2003: Co-founder/Organizer of the 1st Banff Symposium on Organic Chemistry; a student organized event showcasing graduate research across Canada and featuring keynote lectures by internationally renowned chemists. Supported by industrial and academic funding and attended by 120 paid registrants.

2002: Alberta Research Council Graduate Entrance Scholarship for Carbohydrate Chemistry.

1998: Dr. & Mrs. E.M. von Rudloff Bursary. University of Saskatchewan.



PUBLICATIONS:

Sojitra M, Sarkar S, Maghera J, Rodrigues E, Carpenter EJ, Seth S, Ferrer Vinals D, Bennett NJ, Reddy R, Khalil A, Xue X, Bell MR, Zheng RB, Zhang P, Nycholat C, Bailey JJ, Ling CC, Lowary TL, Paulson JC, Macauley MS, Derda R. (2021) Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage. Nat Chem Biol. 17(7):806-816.

Duan S, Arlian BM, Nycholat CM, Wei Y, Tateno H, Smith SA, Macauley MS, Zhu Z, Bochner BS, Paulson JC. (2021) Nanoparticles Displaying Allergen and Siglec-8 Ligands Suppress IgE-FcRI-Mediated Anaphylaxis and Desensitize Mast Cells to Subsequent Antigen Challenge. J Immunol. 206(10):2290-2300.

Bull C, Nason R, Sun L, Van Coillie J, Madriz Sorensen D, Moons SJ, Yang Z, Arbitman S, Fernandes SM, Furukawa S, McBride R, Nycholat CM, Adema GJ, Paulson JC, Schnaar RL, Boltje TJ, Clausen H, Narimatsu Y. (2021) Probing the binding specificities of human Siglecs by cell-based glycan arrays. Proc Natl Acad Sci U S A. 118(17):e2026102118.

Nycholat CM, Duan S, Knuplez E, Worth C, Elich M, Yao A, O'Sullivan J, McBride R, Wei Y, Fernandes SM, Zhu Z, Schnaar RL, Bochner BS, Paulson JC. (2019) A Sulfonamide Sialoside Analogue for Targeting Siglec-8 and -F on Immune Cells. J Am Chem Soc. 141(36):14032-14037.

Wu NC, Lv H, Thompson AJ, Wu DC, Ng WWS, Kadam RU, Lin CW, Nycholat CM, McBride R, Liang W, Paulson JC, Mok CKP, Wilson IA. (2019) Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility. Cell Host Microbe. 25(6):836-844.

Bednar KJ, Nycholat CM, Rao TS, Paulson JC, Fung-Leung WP, Macauley MS. (2019) Exploiting CD22 to Selectively Tolerize Autoantibody Producing B-cells in Rheumatoid Arthritis. ACS Chem Biol. 14(4):644-654.

Edgar LJ, Kawasaki N, Nycholat CM, Paulson JC. (2019) Targeted Delivery of Antigen to Activated CD169+ Macrophages Induces Bias for Expansion of CD8+ T Cells. Cell Chem Biol. 26(1):131-136.

Wu NC, Thompson AJ, Xie J, Lin CW, Nycholat CM, Zhu X, Lerner RA, Paulson JC, Wilson IA. (2018) A complex epistatic network limits the mutational reversibility in the influenza hemagglutinin receptor-binding site. Nat Commun. 9(1):1264.

Sun Y, Hong S, Xie R, Huang R, Lei R, Cheng B, Sun D, Du Y, Nycholat CM, Paulson JC, Chen X. (2018) Mechanistic Investigation and Multiplexing of Liposome-Assisted Metabolic Glycan Labeling. J Am Chem Soc. 140(10):3592-3602.

Spiller F, Nycholat CM, Kikuchi C, Paulson JC, Macauley MS. (2018) Murine Red Blood Cells Lack Ligands for B Cell Siglecs, Allowing Strong Activation by Erythrocyte Surface Antigens. J Immunol. 200(3):949-956.

Wu NC, Zost SJ, Thompson AJ, Oyen D, Nycholat CM, McBride R, Paulson JC, Hensley SE, Wilson IA. (2017) A structural explanation for the low effectiveness of the seasonal influenza H3N2 vaccine. PLoS Pathog. 13(10):e1006682.

Pang L, Macauley MS, Arlian BM, Nycholat CM, Paulson JC. (2017) Encapsulating An Immunosuppressant Enhances Tolerance Induction by Siglec Engaging Tolerogenic Liposomes. Chembiochem. 18(13):1226-1233.

Wu NC, Xie J, Zheng T, Nycholat CM, Grande G, Paulson JC, Lerner RA, Wilson IA. (2017) Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin. Cell Host Microbe. 21(6):742-753.

Wu NC, Xie J, Zheng T, Nycholat CM, Grande G, Paulson JC, Lerner RA, Wilson IA. (2017) Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin. Cell Host Microbe. 22(2):247-248.

Yu H, Gonzalez-Gil A, Wei Y, Fernandes SM, Porell RN, Vajn K, Paulson JC, Nycholat CM, Schnaar RL. (2017) Siglec-8 and Siglec-9 binding specificities and endogenous airway ligand distributions and properties. Glycobiology. 27(7):657-668.

Tzarum N, McBride R, Nycholat CM, Peng W, Paulson JC, Wilson IA. (2017) Unique structural features of influenza H15 HA. J Virol. 91(2):e00046-17.

Guo, H., de Vries, E., McBride, R., Dekkers. J., Peng, W., Bouwman, K.M., Nycholat, C., Verheije, M.H., Paulson, J.C., van Kuppeveld, F.J., de Haan, C.A.M. (2017) Highly Pathogenic Influenza A(H5Nx) Viruses with Altered H5 Receptor-Binding Specificity. Emerg Infect Dis. 23(2):220-231.

Angata, T., Nycholat, C.M., Macauley, M.S. (2015) Therapeutic Targeting of Siglecs using Antibody- and Glycan-Based Approaches. Trends Pharmacol Sci. 36(10):645-60.

Cheng, C.W., Chou, C.C., Hsieh, H.W., Tu, Z., Lin, C.H., Nycholat, C., Fukuda, M., Khoo, K.H. (2015) Efficient Mapping of Sulfated Glycotopes by Negative Ion Mode nanoLC-MS/MS-Based Sulfoglycomic Analysis of Permethylated Glycans. Anal Chem. 87(12):6380-8.

Nycholat, C.M., Peng, W., McBride, R., Antonopoulos, A., de Vries, R.P., Polonskaya, Z., Finn, M.G., Dell, A., Haslam, S.M., and Paulson, J.C. (2013) Synthesis of biologically active N- and O-linked glycans with multi-sialylated poly-N-acetyllactosamine extensions using P. damsela a2-6 sialyltransferase. J Am Chem Soc. 135(49):18280-18283.

Xu, R., de Vries, R.P., Zhu, X., Nycholat, C.M., McBride, B., Yu, W., Paulson, J.C., and Wilson, I.A. (2013) Preferential Recognition of Avian-Like Receptors in Human Influenza A H7N9 Viruses. Science. 342(6163):1230-35.

Macauley, M.S., Pfrengle, F., Rademacher, C., Nycholat, C.M., Gale, A.J., von Drygalski, A., and Paulson, J.C. (2013) Antigenic liposomes displaying CD22 ligands induce antigen-specific B cell apoptosis. J Clin Invest. 123(7):3074-3083.

Kawasaki, N., Vela, J.L., Nycholat, C., Rademacher, C., Khurana, A., van Rooijen, N., Crocker, P.R., Kronenberg, M., and Paulson, J.C. (2013) Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation. Proc Natl Acad Sci U S A. 110(19):7826-31.

Zhu, X., McBride, R., Nycholat, C.M., Yu, W., Paulson, J.C., and Wilson, I.A. (2012) Influenza virus neuraminidases with reduced enzymatic activity that avidly bind sialic acid receptors. J Virol. 86:13371-13383.

Peng, W., Pranskevich, J., Nycholat, C., Gilbert, M., Wakarchuk, W., Paulson, J.C., and Razi, N. (2012) Helicobacter pylori b1,3-N-acetylglucosaminyltransferase for versatile synthesis of type 1 and type 2 poly LacNAcs on N-linked, O-linked and I-antigen glycans. Glycobiology. 22(11):1453-1464.

Bundle, D.R., Nycholat, C., Costello, C., Rennie, R., and Lipinski, T. (2012) Design of a Candida albicans disaccharide conjugate vaccine by reverse engineering a protective monoclonal antibody. ACS Chemical Biology. 7(10): 1754-1763.

Nycholat, C., Rademacher, C., Kawasaki, N., and Paulson, J.C. (2012) In silico aided design of a glycan ligand of sialoadhesin for in vivo targeting of macrophages. J. Am. Chem. Soc. 134:15696-99.

Rhee, J-K., Baksh, M., Nycholat, C., Gilbert, Paulson, J.C., Kitagishi, H., and Finn, MG. (2012) Glycan-Targeted Virus-like Nanoparticles for Photodynamic Therapy. Biomacromolecules. 13(8):2333-8.

Xu, R., Zhu, X., McBride, R., Nycholat, C.M., Yu, W., Paulson, J.C., and Wilson, I.A. (2012) Functional balance of the hemagglutinin and neuraminidase activities accompanies the emergence of the 2009 H1N1 influenza pandemic. J Virol. 86(17):9221-32.

Rademacher, C., Bru, T., McBride, R., Robison, E., Nycholat, C.M., Kremer, E.J., and Paulson, J.C. (2012) A Siglec-like sialic acid binding motif revealed in an adenovirus capsid protein. Glycobiology. 22(8):1086-91.

Chen, W.C., Kawasaki, N., Nycholat, C., Han, S., Pilotte, J., Crocker, P.R., and Paulson, J.C. (2012) Antigen delivery to macrophages using liposomal nanoparticles sargeting Sialoadhesin/CD169. PLoS One. 7(6):e39039.

Nycholat, C.M., McBride, R., Ekiert, D.C., Xu, R., Rangarajan, J., Peng, W., Razi, N., Gilbert, M., Wakarchuk, W., Wilson, I.A., and Paulson, J.C. (2012) Recognition of Sialylated Poly-N-acetyllactosamine Chains on N- and O-Linked Glycans by Human and Avian Influenza A Virus Hemagglutinins. Angew Chem Int Ed Engl. 51(20):4860-63.

Xu, R., McBride, R., Nycholat, C.M., Paulson, J.C. & Wilson, I.A. (2012). Structural characterization of the hemagglutinin receptor specificity from the 2009 H1N1 influenza pandemic. J Virol. 86(2):9.

Rhee, J.K., Hovlid, M., Fiedler, J.D., Brown, S.D., Manzenrieder, F., Kitagishi, H., Nycholat, C., Paulson, J.C. & Finn, M.G. (2011). Colorful Virus-like Particles: Fluorescent Protein Packaging by the Qbeta Capsid. Biomacromolecules. 12(11):3977-81.

Zeng, Y., Rademacher, C., Nycholat, C.M., Futakawa, S., Lemme, K., Ernst, B. & Paulson, J.C. (2011). High affinity sialoside ligands of myelin associated glycoprotein. Bioorg Med Chem Lett. 21, 5045-9.

Nycholat, C. M. & Bundle, D. R. (2009). Synthesis of monodeoxy and mono-O-methyl congeners of methyl-b-D-mannopyranosyl-(1->2)-b-D-mannopyranoside for epitope mapping of anti-Candida albicans antibodies. Carbohydr Res 344, 555-69.

Shukla, A., Nycholat, C., Subramanian, M. V., Anderson, R. J. & Devine, M. D. (2004). Use of resistant ACCase mutants to screen for novel inhibitors against resistant and susceptible forms of ACCase from grass weeds. J Agric Food Chem 52, 5144-50.

Pedras, M. S., Nycholat, C. M., Montaut, S., Xu, Y. & Khan, A. Q. (2002). Chemical defenses of crucifers: elicitation and metabolism of phytoalexins and indole-3-acetonitrile in brown mustard and turnip. Phytochemistry 59, 611-25.


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