The Karbstein Lab
The Karbstein Lab - Publications
Publications
- Ferretti, M.B., Ghalei, H., Ward, E.A., Potts, E.L., and Karbstein, K.* (2017). Rps26 directs mRNA-specific translation by recognition of Kozak sequence elements. NSMB in the press
- Ghalei, H., Trepreau, J., Collins, J.C., Bhaskaran, H., Strunk, B.S. and Karbstein, K. (2017) The ATPase Fap7 Tests the Ability to Carry Out Translocation-Like Conformational Changes and Releases Dim1 During 40S Ribosome Maturation. Mol. Cell PMID: 28890337
- Johnson, M.C., Ghalei, H., Doxtader, K.A., Karbstein, K., Stroupe, M.E. (2017) Structural heterogeneity in Pre-40S Ribosomes Structure. 2017 25(2):329-340. PMCID: PMC5314460. [Preview by Hartwick & Wimberly (2017) Structure 25(2)].
- Khoshnevis, S., Askenazy, I., Johnson, M.C., Dattolo, M.D., Young-Erdos, C.L., Stroupe, M.E. & Karbstein, K. The DEAD-box protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation. PLoS Biol. 2016 Jun 9;14(6):e1002480. doi: 10.1371/journal.pbio.1002480. eCollection 2016 Jun. PMCID: PMC4900678
- Karbstein, K.*, What Will the Future Hold? RNP quality Control and Degradation. (2015) RNA 21(4):657-658. PMCID: PMC4371321
- De la Cruz, J.*, Karbstein, K.* and Woolford, J.L.*, Functions of Ribosomal Proteins in Assembly of Eukaryotic Ribosomes In Vivo. (2015) Annual Reviews Biochemistry 84: 93-129 * corresponding authors. PMCID: PMC4772166
- Tyagi R., Shahani N., Gorgen L., Ferretti M., Pryor W., Chen P.Y., Swarnkar S., Worley P.F., Karbstein, K., Snyder, S.H., Subramaniam, S. Rheb Inhibits Protein Synthesis by Activating the PERK-elF2α Signaling Cascade. (2015) Cell Reports 10(5) 684-693. PMCID: PMC4524804
- Ghalei, H., Schaub, F.X., Doherty, J.R., Noguchi, Y., Roush, W.R., Cleveland, J.L., Stroupe, M.E. & Karbstein, K. (2015). Hrr25/CK1d-directed release of Ltv1 from pre-40S ribosomes is necessary for ribosome assembly and cell growth. Journal of Cell Biology 208(6):745-59. PMCID: PMC4362465
- Young CL, Khoshnevis S, Karbstein K. (2013) Cofactor-dependent specificity of a DEAD-box protein, Proc Natl Acad Sci USA. PMCID: PMC3718167
- Karbstein, K. (2013) Quality control mechanisms during ribosome maturation, Trends Cell Biol. 23(5): 242-50. PMCID: PMC3640646
- Strunk B.S., Karbstein K. (2012) A translation-like cycle is a quality for control checkpoint for maturing 40S ribosome subunits, Cell 150, 111-121. PMCID: PMC3615461
- Young C.L., Karbstein K. (2012) Analysis of cofactor effects on RNA helicases. Methods in Enzymology 511: 213-37.
- Karbstein K. (2011) Inside the 40S ribosome assembly machinery. Curr Opin Chem Biol. Oct;15(5) 657-63. PMCID: PMC3329787
- Horn DM, Mason SL, Karbstein K. (2011) RCL1, a novel nuclease for 18S rRNA production. J Biol Chem. PMCID: PMC3190816
- Strunk BS, Loucks CR, Su M, Vashisth H, Cheng S, Schilling J, Brooks CL 3rd, Karbstein K, Skiniotis G. (2011) Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates. Science.
- Young, C.L. and Karbstein, K (2011) The Roles of S1 RNA-Binding Domains in Rrp5’s Interactions with pre-rRNA. RNA 17, 512-521. PMCID: PMC3039150
- Campbell, M.G. and Karbstein, K. (2011) Protein - Protein Interactions within Late Pre-40S Ribosomes. PloS ONE 6, e16194
- Woolls, H., Lamanna, A.C. and Karbstein, K. (2010) Roles of DIM2 Ribosome Assembly. J. Biol. Chem. 286, 2578-2586. PMCID: PMC3024753
- Lamanna, A.C. and Karbstein, K. (2011) An RNA Conformational Switch Regulates Pre-18S rRNA Cleavage. J. Mol. Biol. 405, 3-17
- Karbstein, K. (2010) Chaperoning Ribosome Assembly. J. Cell Biol. 181, 11-12.
- Strunk, B.S. and Karbstein, K. (2009) Powering Through Ribosome Assembly. RNA 15, 2083-2104.
- Lamanna, A.C. and Karbstein, K. (2009) Nob1 Binds the Single-Stranded Cleavage Site D at the 3’-end of 18S rRNA with its PIN Domain. Proceedings of the National Academy of Sciences USA 106, 14259-14264. PMCID: PMC3006654
- Karbstein, K. (2009) Eukaryotic Ribosome Assembly. Wiley Encyclopedia of Chemical Biology 4, 222-230.
- Karbstein, K. (2007) GTPases in Ribosome Assembly. Biopolymers 87, 1-11.
- Karbstein, K., Lee, J. and Herschlag, D. (2007) Probing the Role of a Secondary Structure Element at the 5’- and 3’- Splice Sites in Group I Intron Self-Splicing: The Tetrahymena L-16 ScaI Ribozyme Reveals a new Role of the G.UPair in Self-Splicing. Biochemistry 46, 4861-4875.
- Karbstein, K. and Doudna, J.A. (2006) GTP-dependent Formation of a Ribonucleoprotein Subcomplex Required for Ribosome Biogenesis. Journal of Molecular Biology 356, 432-443.
- Karbstein, K., Jonas, S. and Doudna, J.A. (2005) An Essential GTPase Promotes Assembly of Ribosomal Processing Complexes. Molecular Cell 20, 633-643. [Preview by Dutca & Culver Molecular Cell 20, 497livepage.apple.com-499]
- Karbstein, K. and Doudna (2004) RNA: Primed for Packing? Chemistry & Biology 11, 149-151.
- Karbstein, K., Tang, K.H. and Herschlag, D. (2004) A Base Triple in the Tetrahymena Group I Core Affects the Reaction Equilibrium via a Threshold Effect. RNA 10, 1730-1739.
- Karbstein, K. and Herschlag, D. (2003) Extraordinarily Slow Binding of Guanosine to the TetrahymenaGroup I Ribozyme: Imlications for RNA Preorganization and Function. Proceedings of the National Academy of Sciences 100, 2300-2305.
- Karbstein, K., Carroll, K.S. and Herschlag, D. (2002) Probing the Tetrahymena Group I Ribozyme Reaction in Both Directions. Biochemistry 41, 11171-11183.
- Wang, S.L., Karbstein, K., Peracchi, A., Beigelman, L. and Herschlag, D. (1999) Identification of the Hammerhead Ribozyme Metal Ion Bindng Site that Rescues the Deleterious Effect of a cleavage Site Phosphorothioate. Biochemistry 38, 14363-14378.
Announcements
Max wins Best Poster at the Scripps Research Fest! Graduate Student, Haina Huang, joins the lab! Welcome Haina!
Congratulations to Homa on her position as Assistant Professor in the Department of Biochemistry at Emory University (https://www.biochem.emory.edu/) on October 1, 2017.
Katrin is named an HHMI Faculty Scholar! 
Miriam Koch joins the lab! Welcome Miriam!
Nickan Khoshnevis is born! Congratulations Homa and Sohail!
Joanne Doherty joins the lab as Staff Scientist! Welcome Joanne!
Graduate Student, Melissa Parker, joins the lab! Welcome Melissa!
Jason Collins joins the lab! Welcome Jason!
Sohail's poster wins best poster prize at Research Fest!
Ribosome Assembly Meeting in Brussels, August 19-23, 2015! http://ribosome.scripps.edu
Graduate Student, Lizz Miguet, joins the lab! Welcome Lizz!
Scripps awarded an NSF REU grant for undergraduate research.
Katrin wins tenure!
Homa's poster wins prize at the Scripps Research Fest!
Homa's poster wins prize at the Ribosome Meeting!
Graduate Student, Max Ferretti, joins the lab! Welcome Max!
