Scripps Florida Logo

The Cell Adhesion Laboratory

Publications - last updated January 21, 2020

Janiszewska M, Primi, MC, Izard T (2020)
Cell adhesion in cancer: Beyond the migration of single cells
Journal of Biological Chemistry 10.1074/jbc.REV119.007759

Mediouni S, Chinthalapudi K, Ekka MK, Usui I, Jablonksi JA, Clementz MA, Mousseau G, Nowak J, Macherla VR, Beverage JN, Esquenazi E, Baran P, de Vera IMS, Kojetin D, Loret EP, Nettles K, Maiti S, Izard T, Valenti ST (2019)
Didehydro-cortistatin A inhibits HIV-1 by specifically binding to the unstructured basic region of Tat
MBio 10:e02662

Valencia-Gallardo C, Bou-Nader C, Aguilar-Salvador DI, Carayol N, Quenech'Du N, Pecquer L, Park H, Fontecave M, Izard T, Tran Van Nhieu G (2019)
Shigella IpaA binding to talin stimulates filopodial capture and cell adhesion
Cell Rep 26:921-932

Patil DN, Rangarajan ES, Novick SJ, Pascal BD, Kojetin DJ, Griffin PR, Izard T, Martemyanov KA (2018)
Structural organization of a major neuronal G protein regulator, the RGS7-Gβ5-R7BP complex
eLIFE 7:e42150

Chinthalapudi K, Rangarajan ES, Izard T* (2018)
The interaction of talin with the cell membrane is essential for integrin activation and focal adhesion formation
Proc Natl Acad Sci U S A  115:10339-10344

Chinthalapudi K, Mandati V, Zheng J, Sharff AJ, Bricogne G, Griffin PR, Kissil J, Izard T* (2018)
Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2
Nature 9:1338

Stender JD, Nwachukwu JC, Kastrati I, Kim Y, Strid T, Yakir M, Srinivasan S, Nowak J, Izard T, Rangarajan ES, Carlson KE, Katzenellenbogen JA, Yao XQ, Grant BJ, Leong HS, Lin CYFrasor JNettles KW, Glass CK (2017)
Structural and molecular mechanisms of cytokine-mediated endocrine resistance in human breast cancer cells 
Molecular Cell  65:1122-1135

Nwachukwu JC, Srinivasan S, Bruno NE, Nowak J, Wright NJ, Minutolo F, Rangarajan ES, Izard T, Yao XQ, Grant BJ, Kojetin DJ, Elemento O, Katzenellenbogen JA, Nettles KW (2017)
Systems structural biology analysis of ligand effects on ERα predicts cellular response to environmental estrogens and anti-hormone therapies
Cell Chemical Biology  24:35-45

Cao W, Kayama H, Chen ML, Delmas A, Sun A, Kim SY, Rangarajan ES, McKevitt K, Beck AP, Jackson CB, Crynen G, Oikonomopoulous A, Lacey PN, Martinez GJ, Izard T, Lorenz RG, Rodriguez-Palacios A, Cominelli F, Abreu MT, Hommes DW, Koralov SB, Takeda K, Sundrud MS (2017)
The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids
Immunity 47:1182-1196

Stender JD, Nwachukwu JC, Kastrati I, Kim Y, Strid T, Yakir M, Srinivasan S, Nowak J, Izard T, Rangarajan ES, Carlson KE, Katzenellenbogen JA, Yao XQ, Grant BJ, Leong HS, Lin CY, Frasor J, Nettles KW, Glass CK (2017)
Structural and Molecular Mechanisms of Cytokine-Mediated Endocrine Resistance in Human Breast Cancer Cells
Molecular Cell 65:1122-1135

Nwachukwu JC, Srinivasan S, Bruno NE, Nowak J, Wright NJ, Minutolo F, Rangarajan ES, Izard T, Yao XQ, Grant BJ, Kojetin DJ, Elemento O, Katzenellenbogen JA, Nettles KW (2017)
Systems Structural Biology Analysis of Ligand Effects on ERα Predicts Cellular Response to Environmental Estrogens and Anti-hormone Therapies
Cell Chem Biology 24:35-45

Chinthalapudi K, Rangarajan ES, Brown DT, Izard T* (2016)
Differential lipid binding of vinculin isoforms promotes quasi-equivalent dimerization
Proc Natl Acad Sci U S A 113:9539-9544

Izard T, Brown DT (2016)
Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites
Journal of Biological Chemsitry 291:2548-2555

Brown DT,  Izard T* (2015)
Vinculin-cell membrane interactions
Oncotarget 6:34043-34044

Chinthalapudi K, Patil D, Rangarajan E, Rader C, Izard T* (2015)
Lipid-directed vinculin dimerization
Biochemistry 54:2758-2768

Chinthalapudi K, Rangarajan E, Patil D, George E, Brown DT, Izard T* (2014)
Lipid binding promotes oligomerization and focal adhesion activity of vinculin
Journal of Cell Biology 207:643-656

Chinthalapudi K, Rangarajan E, Patil D, Izard T* (2014)
Lipid-directed cytoskeletal protein oligomerization at sites of cell adhesion
Acta Crystallographica A70:C1833

Poussin K, Pilati C, Couchy G, Calderaro J, Bioulac-Sage P, Bacq Y, Paradis V, Leteurtre E, Sturm N, Ramos J, Guettier C, Bardier-Dupas A, Boulai A, Wendum D, Selves J, Izard T, Nault JC, Zucman-Rossi J (2013)
Biochemical and functional analyses of gp130 mutants unveil JAK1 as a novel therapeutic target in human inflammatory hepatocelluar adenoma
Oncoimmunology 12:e27090

Lee JH, Vonrhein C, Bricogne G, Izard T* (2013)
Crystal structure of the N-terminal domains of the surface cell antigen 4 of Rickettsia
Protein Science 10:1425-1431

Rangarajan ES, Izard T* (2013)
Dimer asymmetry defines α-catenin interactions
Nature Structural Molecular Biology 20:188-193

Lee JH, Rangarajan ES, Vonrhein C, Bricogne G, Izard T* (2012)
The metavinculin tail domain directs constitutive interactions with raver1 and vinculin RNA
Journal of Molecular Biology 422:697-704

Rangarajan ES, Izard T* (2012)
The Cytoskeletal Protein α-Catenin unfurls upon binding to vinculin
Journal of Biological Chemistry 287:18492-18499

Yogesha SD, Rangarajan ES, Vonrhein C, Bricogne G, Izard T* (2012)
Crystal structure of vinculin in complex with vinculin binding site 50 (VBS50), the integrin binding site 2 (IBS2) of talin
Protein Science 21:583-588

Yogesha SD, Sharff AJ, Giovannini M, Bricogne G, Izard T* (2011)
Unfurling of the band 4.1, ezrin, radixin, moesin (FERM) domain of the merlin tumor suppressor
Protein Science 20:2113-2120

Park H, Lee JH, Gouin E, Cossart P, Izard T* (2011)
The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin
Journal of Biological Chemistry 286:35096-35103

Pilati C, Amessou M, Bihl MP, Balabaud C, Nhieu JT, Paradis V, Nault JC, Izard T, Bioulac-Sage P, Couchy G, Poussin K, Zucman-Rossi J (2011)
Somatic mutations activating STAT3 in human inflammatory hepatocellular adenomas
Journal of Experimental Medicine 208:1359-1366

Yogesha SD, Sharff A, Bricogne G, Izard T* (2011)
Intermolecular versus intramolecular interactions of the vinculin binding site 33 of talin
Protein Science 20:1471-1476

Rangarajan ES, Lee JH, Izard T* (2011) 
Apo raver1 structure reveals distinct RRM domain orientations
Protein Science 20:1464-1470

Park H, Valencia-Gallardo C, Sharff A, Tran Van Nhieu G, Izard T* (2011)
Novel vinculin binding site of the IpaA invasin of Shigella
Journal of Biological Chemistry 286:23214-23221

Rangarajan ES, Izard T* (2010) 
Improving the diffraction of full-length human selenomethionyl metavinculin crystals by streak-seeding
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:1617-1620

Rangarajan ES, Lee JH, Yogesha SD, Izard T* (2010)
A helix replacement mechanism directs metavinculin functions
PLoS One 5:e10679

Park H, Rangarajan ES, Sygusch J, Izard T (2010)
Dramatic improvement of crystal quality for low-temperature-grown rabbit muscle aldolase
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:595-600

George EM, Izard T, Anderson SD, Brown DT (2010)
The nucleosome interaction surface of linker histone H1c is distinct from that of H10
Journal of Biological Chemistry 285:20891-20896

Rangarajan ES, Park H, Fortin E, Sygusch J, Izard T (2010)
Mechanism of aldolase control of sorting nexin 9 function in endocytosis
Journal of Biological Chemistry 285:11983-11990

Lee JH, Rangarajan ES, Yogesha SD, Izard T* (2009)
Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions
Structure 17:833-842

Rebouissou S, Amessou M, Couchy G, Poussin K, Imbeaud S, Pilati C, Izard T, Balabaud C, Bioulac-Sage P, Zucman-Rossi J (2009)
Frequent in-frame somatic deletions activate gp130 in inflammatory hepatocellular tumours
Nature 457:200-204

Nhieu GT, Izard T* (2007)
Vinculin binding in its closed conformation by a helix addition mechanism
EMBO J 26:4588-4596

St-Jean M, Izard T, Sygusch J (2007)
A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein
Journal of Biological Chemistry 282:14309-14315

Ramarao N, Le Clainche C, Izard T, Bourdet-Sicard R, Ageron E, Sansonetti PJ, Carlier MF, Tran Van Nhieu, G (2007)
Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain
FEBS Letters 581:853-857

Izard T*, Tran Van Nhieu G, Bois PR (2006)
Shigella applies molecular mimicry to subvert vinculin and invade host cells
Journal of Cell Biology 175:465-475

Bois PR, O'Hara BP, Nietlispach D, Kirkpatrick J, Izard T* (2006)
The vinculin binding sites of talin and α-actinin are sufficient to activate vinculin
Journal of Biological Chemistry 281:7228-7236

Brown DT, Izard T, Misteli T (2006)
Mapping the interaction surface of linker histone H1(0) with the nucleosome of native chromatin in vivo
Nature Structural & Molecular Biology 13:250-255

Bois PRJ, Borgon RA, Vonrhein C, Izard T* (2005)
Structural dynamics of α-actinin-vinculin interactions
Molecular and Cellular Biology 25:6112-6122 

Morris VK, Izard T* (2004)
Substrate-induced asymmetry and channel closure revealed by the apoenzyme structure of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase
Protein Science 13:2547-2552

Borgon RA, Vonrhein C, Bricogne G, Bois PRJ, Izard T* (2004)
Crystal structure of human vinculin
Structure 12:1189-1197

Izard T*, Vonrhein C (2004)
Structural basis for amplifying vinculin activation by talin
Journal of Biological Chemistry 279:27667-27678

Rush CL, Izard T* (2004)
Rhombohedral crystals of the human vinculin head domain in complex with a vinculin-binding site of talin
Acta Crystallographica D60:945-947

Izard T*, Evans G, Borgon RA, Rush CL, Bricogne G, Bois PRJ (2004)
Vinculin activation by talin through helical bundle conversion
Nature 427:171-175

Izard T, Sygusch J. (2004)
Induced fit movements and metal cofactor selectivity of class II aldolases: Structure of thermus aquaticus fructose-1,6-bisphosphate aldolase
Journal of Biological Chemistry 279:11825-11822

Brown KL, Morris VK, Izard T* (2004)
Rhombohedral crystals of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase
Acta Crystallographica D60:195-196

Izard T* (2003)
A novel adenylate binding site confers phosphopantetheine adenylyltransferase interactions with Coenzyme A
Journal of Bacteriology 185:4174-4080

Hurlbert J, Izard T* (2002)
Crystallization of HLA-DR4 complexed with a collagen II peptide
Acta Crystallographica D58:1749-1751

Izard T* (2002)
The crystal structures of phosphopantetheine adenylyltransferase with bound substrates reveal the enzyme's catalytic mechanism
Journal of Molecular Biology 315:487-495

Izard T* (2001)
Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activity
Protein Science 10:1508-1513

Izard T*, Blackwell N (2000)
Crystal structures of the metal-dependent 2-dehydro-3-deoxy-galactarate aldolase suggest a novel reaction mechanism
The EMBO Journal 19:3849-3856

Izard T*, Ellis J (2000)
The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism
The EMBO Journal 19:2690-2700

Blackwell NC, Cullis PM, Cooper RA, Izard T* (1999)
Rhombohedral crystals of 2-dehydro-3-deoxy glucarate aldolase Escherichia coli
Acta Crystallographica D55:1368-1369

Izard T*, Geerlof A, Lewendon A, Barker JJ (1999)
Cubic crystals of phosphopantetheine adenylyltransferase from Escherichia coli
Acta Crystallographica D55:1226-1228

Ellis J, Campopiano DJ, Izard T* (1999)
Cubic crystals of chloramphenicol phosphotransferase from Streptomyces venezuelae
Acta Crystallographica D55:1086-1088

Izard T*, Geerlof A (1999)
The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivity
The EMBO Journal 18:2021-2030

Izard T, Aevarsson A, Allen MD, Westphal AH, Perham RN, de Kok A, Hol WGJ (1999)
Principles of quasi-equivalence and euclidean geometry govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes
Proceedings of the National Academy of Sciences USA 96:1240-1245

Izard T, Sarfaty S, de Kok A, Westphal A, Hol WGJ (1997)
Improvement of diffraction quality upon rehydration of dehydrated icosahedral E. faecalis pyruvate dehydrogenase core crystals
Protein Science 6:913-915

Izard T, Lawrence MC, Malby RL, Lilley, GG, Colman PM (1994)
The three-dimensional structure of N-acetylneuraminate lyase from Escherichia coli
Structure 2:361-369

Lawrence MC, Izard T, Beuchat M, Blagrove RJ, Colman PM (1994)
The structure of phaseolin at 2.2 Å resolution: Implications for a common vicilin/legumin structure and the genetic engineering of seed storage proteins
Journal of Molecular Biology 238:748-776

Izard T, Fol B, Pauptit RA, Jansonius JN (1990)
Trigonal crystals of porcine mitochondrial aspartate aminotransferase
Journal of Molecular Biology 215:341-344

PhD Thesis: "Three Dimensional Fourier Synthesis of N-neuraminate Lyase from Escherichia coli"