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Zinc Finger Tools
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ZF Tools home
Intro to ZFPs
Tools overview
DNA search tips
ZFP design tips
Available triplets
Synthesize a ZFP
Effector domains
Nuclease design
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Search DNA Sequence for Contiguous or Separated
Target Sites

Enter a DNA sequence to be scanned for target sites (10 kb max).

Triplets to search
ZF set
Search both strands
  Contiguous targets
  Separated targets
Minimum target size (bp)
Half-site size (bp)
Core (IUPAC bases or number of bp)
JuxtaposeC-terms or N-terms

Design a Zinc Finger Protein

Enter the DNA target site. The ZF amino acid sequence predicted to recognize this site
will be determined. This tool expects only valid triplets.

Target site 5' 3'
N-term backbone N-term fixed
C-term backbone C-term fixed
Zinc finger linker Include fixed sequences? 

Predict Zinc Finger Protein DNA Binding Site

Enter the ZF single letter amino acid sequence (1500 residues max).
Recognizes only certain published helices.

Search DNA Sequence and Target Site for Close Match

Enter DNA sequence to be searched (10 kb max).

Target site Number mismatches Search both strands
IUPAC bases

While every effort has been made to ensure the accuracy of the information reported on this site, we make no guarantees
and strongly suggest that you double check all sequences and information obtained here before using them.

ZF Tools Ver 3.0
Created by Jeff Mandell for the Barbas Laboratory at TSRI.
This site should be used with IE 6.0 or Firefox on Windows and Safari on the Mac. Functionality may be compromised with Netscape.