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The Wüthrich Laboratory

Photo of Michael Geralt
Michael Geralt

Research Technician

mgeralt@scripps.edu
phone: +1 858 784 2084
fax     : +1 858 784 8014 
 
 

 

Reserach Interests:

As Laboratory Manager and Safety Officer my duties entail maintaining a safe, clean and functional working environment for all of the post doctorate and graduate students that work in the group. My other responsibilities in the laboratory involve the handling and production of proteins specific to the group’s research focus.  Currently I am involved in 2 efforts:  the production of high quality NMR samples of kappa opioid receptor for functional and dynamic studies; and the production and study of mechanisms of RNA-binding motifs.       

 Curriculum Vitae:

2010 - Present:  Research Assistant III (The Scripps Research Institute, La Jolla, CA).
2005 - 2010:  Research Assistant I (The Scripps Research Institute, La Jolla, CA).
2003 - 2005:  Research Technician (The Scripps Research Institute, La Jolla, CA).
2002 - 2003:  Student Lab Associate (Salk Institute for Biological Studies, La Jolla, CA).
2003: Bachelor of Science w/ emphasis in cellular and molecular biology (San Diego State University, San Diego, CA). 

Publications:

  1. Stepanyuk, GA; Serrano P; Peralta E; Farr CL; Axelrod HL; Geralt M; Das D; Chiu HJ; Jaroszewski L; Deacon AM; Lesley SA; Elsliger MA; Godzik A; Wilson IA; Wüthrich K; Salomon DR; Williamson JR. (2016) Acta Crystallogr D Struct Biol. 72: 497-511, UHM-ULM interactions in the RBM-U2AF65 splicing-factor complex.
  1. Proudfoot, A.; Axelrod H.L.; Geralt, M.; Fletterick R.J.; Yumoto, F.; Deacon A.M.; Elsliger, M.A.; Wilson, I.A.; Wüthrich, K.; Serrano, P. (2016) J. Mol. Biol. 428: 1130-1141 Dlx5 Homeodomain:DNA Complex:Structure, Binding and Effect of Mutations Related to Split Hand and Foot Malformation Syndrome.
  1. Mohanty, B.; Geralt, M.; Wüthrich, K.; Serrano, P. (2016) Protein Sci. 25: 917-925, NMR reveals structural rearrangements associated to substrate insertion in nucleotide-adding enzymes.
  1. Martin, B.T.; Serrano, P.; Geralt, M.; Wüthrich, K. (2016) Structure 24: 158-164, Nuclear Magnetic Resonance Structure of a Novel Globular Domain in RBM10 Containing OCRE, the Octamer Repeat Sequence Motif.
  1. Dutta, S.K.; Serrano, P.; Geralt, M.; Axelrod, H.L.; Xu, Q.; Lesley, S.A.; Godzik, A.; Deacon, A.M.; Elsliger, M.A.; Wilson, I.A.; Wüthrich, K. (2015) Protein Sci. 24: 1600-1608 Cofactor-induced reversible folding of Flavodoxin-4 from Lactobacillus acidophilus.
  1. Mohanty, B.; Serrano, P.; Geralt, M.; Wüthrich, K. (2015) 61: 83-87, NMR structure determination of the protein NP_344798.1 as the first representative of Pfam PF06042.
  1. Jaudzems, K.; Pedrini, B.; Geralt, M.; Serrano, P.; Wüthrich, K. (2015) J. Biomol. NMR 61: 65-72, J-UNIO protocol used for NMR structure determination of the 206-residue protein NP_346487.1 from Streptococcus pneumoniae TIGR4.
  1. Dutta, S.K.; Serrano, P.; Proudfoot, A.; Geralt, M.; Pedrini, B.; Herrmann, T.; Wüthrich, K. (2015) J. Biomol. NMR 61: 47-53, APSY-NMR for protein backbone assignment in high-throughput structural biology.
  1. Serrano, P.; Geralt, M.; Mohanty, B.; Wüthrich, K. (2014) J. Mol. Biol. 426: 2547-2553, NMR Structures of α-Proteobacterial ATPase-Regulating ζ-Subunits.
  1. Pedrini, B.; Serrano, P.; Mohanty, B.; Geralt, M.; Wüthrich, K. (2013) Biopolymers 99: 825-831, NMR-profiles of protein solutions.
  1. Serrano, P.; Geralt, M.; Mohanty, B.; Wüthrich, K. (2013) Protein Sci, 7: 1000-1007, Structural Representative of the Protein Family PF14466 Has a New Fold and Establishes Links with the C2 and PLAT Domains From the Widely Distant Pfams PF00168 and PF01477.
  1. Geralt, M.; Alimenti, C.; Vallesi, A.; Luporini, P.; Wüthrich, K. (2013) Biology , 2:142-250, Thermodynamic Stability of Psychrophilic and Mesophilic Pheromones of the Protozoan Ciliate Euplotes.
  1. Serrano, P., Pedrini, B., Mohanty, B., Geralt, M., Herrmann, T., Wüthrich, K. (2012) J. Biomol NMR, 53:341-354, The J-UNIO protocol for automated protein structure determination by NMR in solution.
  1. Wahab, A.T., Serrano, P., Geralt, M. and Wüthrich, K. (2011) Protein Science 20, 1137–1144. NMR structure of the Bordetella bronchiseptica protein NP_888769.1 establishes a new phage-related protein family PF13554.
  1.  Jaudzems, K., Geralt, M., Serrano, P., Mohanty, B., Horst, R., Pedrini, B., Elsliger, M-A., Wilson, M-A., Wüthrich, K. (2010) Acta Cryst. F66, 1367-1380, NMR structure of the protein NP_247299.1: Comparison with the crystal structure.
  1. Mohanty, B., Serrano, P., Pedrini, B., Jaudzems, K., Geralt, M., Horst, R., Herrmann, T., Elsliger, M.-A., Wilson, I. A., Wüthrich, K. (2010) Acta Cryst. F66, 1381-1392. Comparison of NMR and crystal structures for the proteins TM1112 and TM1367.
  2. Serrano, P., Pedrini, B., Geralt, M., Jaudzems, K., Mohanty, B., Horst, R., Herrmann, T., Elsliger, M.-A., Wilson, I. A., Wüthrich, K. (2010) Acta Cryst. F66, 1393 - 1405. Comparison of NMR and Crystal Structures Highlights Conformational Isomerism in Protein Active Sites.
  3. Zhang, Q., Horst, R., Geralt, M., Ma, X., Hong, W., Finn, M.G., Stevens, R., Wüthrich, K. (2008) J. Am. Chem. Soc. 130, 7357–7363. Microscale NMR screening of new detergents for membrane protein structural biology.
  4. Almeida, M.S., Johnson, M.A., Herrmann, T., Geralt, M., Wüthrich, K. (2007) J.Virol .81, 3151–3161. Novel β-barrel fold in the nuclear magnetic resonance structure of the replicase nonstructural protein 1 from the severe acute respiratory syndrome coronavirus.
  5. José M. Alonso, Anna N. Stepanova, Thomas J. Leisse, Christopher J. Kim, Huaming Chen, Paul Shinn, Denise K. Stevenson, Justin Zimmerman, Pascual Barajas, Rosa Cheuk, Carmelita Gadrinab, Collen Heller, Albert Jeske, Eric Koesema, Cristina C. Meyers, Holly Parker, Lance Prednis, Yasser Ansari, Nathan Choy, Hashim Deen, Michael Geralt, Nisha Hazari, Emily Hom, Meagan Karnes, Celene Mulholland, Ral Ndubaku, Ian Schmidt, Plinio Guzman, Laura Aguilar-Henonin, Markus Schmid, Detlef Weigel, David E. Carter, Trudy Marchand, Eddy Risseeuw, Debra Brogden, Albana Zeko, William L. Crosby, Charles C. Berry, and Joseph R. Ecker (2003) Science 301: 653-657 Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana.