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Shen Lab Research: Project 4

(iv) Expression of natural product biosynthetic gene clusters in heterologous hosts for natural product production and development of new strategies for combinatorial biosynthesis

1. Expression of the macrotetrolide biosynthetic gene cluster from Streptomyces griseus in Streptomyces  lividans for macrotetrolide production (~ 50 mg/L) (J. Am. Chem. Soc., 2001, 123, 3385-3386)

Image of nonactin

2. Expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus in Streptomyces albus for fredericamycin A and E production (J. Am. Chem. Soc. 2005, 127, 16442-16452; J. Bacteriol. 2008, 190, 5587-5596)

Image of Fredericamycin E and A

3. Expression of the iso-migrastatin biosynthetic gene cluster from Streptomyces platensis in selected Streptomyces species (Bioorg. Med. Chem. 2009, 17, 2147-2159; Appl. Microbiol. Biotechnol. 2011, 89, in press)

iso-Migrastatin