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Shen Lab Research: Project 1

(i) Cloning and characterization of novel natural product biosynthetic gene clusters and machinery

1. The bleomycin biosynthetic gene cluster from Streptomyces verticillus ATCC15003 (Chem. Biol. 1999, 6, 507-517; Chem. Biol. 2000, 7, 623-642; Reviews: Bioorg. Chem. 1999, 27, 155-171; J. Ind. Microbiol. Biotechnol. 2001, 27, 378-385; J. Nat. Prod. 2002, 65, 422-531; J. Nat. Prod. 2011, 74, in press)

Image of biosynthetic gene cluster

2. The C-1027 biosynthetic gene cluster from Streptomyces globisporus (Antimicrob. Agents Chemother. 2000, 44, 382-392; Science 2002, 297, 1170-1173; Review: Bioorg. Chem. 1999, 27, 172-188; Curr. Med. Chem. 2003, 10, 2317-2325; Curr. Topics med. Chem. 2008, 8, 448-459)

Image of biosynthetic gene cluster

3. The macrotetrolide biosynthetic gene cluster from Streptomyces griseus DSM40695 (Antimicrob. Agents Chemother. 2000, 44, 1943-1953; J. Am. Che. Soc. 2001, 123, 3385-3386; Science 2002, 297, 1327-1330; Review: Chem. Rec. 2002, 2, 389-396)

Image of macrotetrolide biosynthetic gene cluster

4. The leinamycin biosynthetic gene cluster from Streptomyces atroolivaceus (J. Bacteriol. 2002, 184, 7013-7024; Proc. Natl. Acad. Sci. 2003, 100, 3010-3012; Chem. Biol. 2004, 11, 33-45)

Image of Leinamycin

5. The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC15944 (Nat. Biotechnol. 2003, 21, 187-190; Chem. Biol. 2005, 12, 293-302)

Image of Neocarzinostatin

6. The maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC39144 (Proc. Natl. Acad. Sci. 2003, 100, 11959-11963; J. Am. Chem. Soc. 2007, 129, 13082-13094)

Image of Maduropeptin

7. The fredericamycin biosynthetic gene cluster from Streptomyces griseus (J. Am. Chem. Soc. 2005, 127, 16442-16452)

Image of Fredericamycin A

8. The tautomycin biosynthetic gene cluster from Streptomyces spiroverticillus (J. Bacteriol. 2006, 198, 4148-4152; J. Biol. Chem. 2008, 283, 28607-28617)

Image of Tautomycin

9. The oxazolomycin biosynthetic gene cluster from Streptomyces albus (J. Bacteriol. 2006, 198, 4141-4147; J. Biol. Chem. 2010, 285, 20097-20108)

Image of Oxazolomycin

10. The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94 ATCC31158 (Mol. BioSyst. 2007, 3, 60-74)

Image of Tallysomycins

11. The zorbamycin biosynthetic gene cluster from Streptomyces flavoviridis ATCC21892 (Mol. BioSyst. 2008, 4, 77-90)

Image of Zorbamycin

12. The platensimycin and platencin biosynthetic gene cluster from Streptomyces MA7327 (Antimicro. Agents Chermother. 2009, 53, 1299-1304)

Image of Platensimycin and Platencin

13. The tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes (J. Nat. Prod. 2009, 72, 450-459)

Imageo f Tautomycetin

14. The iso-migrastatin, migrastatin, and dorrigocin biosynthetic gene cluster from Streptomyces platensis NRRL18993 (J. Biol. Chem. 2009, 284, 28746-29756)

Image of iso-Migrastatin, Migrastatin, and Dorrigocin A