Publications

The following articles were published by Dr. Shen, his lab members and his collaborators. To jump to a specific year, please click on link below:
2012|2011|2010|2009|2008|2007|2006|2005|2004|2003|2002|2001|2000|1999|1991-1996|1986-1990

Publications 2013

Lohman, J. R.; Huang, S.-X.; Horsman, G.P.; Dilfer, P.; Huang, T.; Chen, Y.; Wendt-Pienkowski, E.; Shen, B. (2013) Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics.  Mol. BioSyst. 9:in presss.

Lohman, J. R,; Bingman, C. A.; Phillips Jr., G. N.; Shen, B. (2013) The structure of the bifunctional acyltransferase/decarboxylase LnmK from the leinamycin biosynthetic pathway revealing novel activity for a double hot dog fold.  Biochemistry 52.  [PubMed]

Unsin, C. E.-M.; Rajski, S. R.; Shen, B. (2013) “The Role of Genetic Engineering in Natural Product-based Anticancer Drug Discovery” in Natural Products and Cancer Drug Discovery, Koehn, F. E. ed., Springer, New York, NY, pp175-191.

Kurata, S.; Shen, B.; Liu, J. O.; Takeuchi, N.; Kaji, A.; Kaji, H. (2013) Possible steps of complete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors. Nucleic Acids Res. 41:264-276.

Van der Donk, et al. (total of 58 co-authors). (2013) Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Nat. Prod. Rep. 30:108-160.

Yu, Z.; Rateb, M. E.; Smanski, M. J.; Peterson, R. M.; Shen, B. (2013) Isolation and structural elucidation of glucoside congeners of platencin from Streptomyces platensis SB12600. J. Antibiot. 66:in press.

Publications 2012 

Yu, Z.; Vodanovic-Jankovic, S.; Kron, M.; Shen, B. (2012) New WS9326A Congeners from Streptomyces sp. 9078 inhibiting Brugia malayi asparaginyl-tRNA synthetase.  Org. Lett. 14:4946-4949. [PubMed] 

Lee, S.; Liu, B.; Lee, S.; Huang, S.-X.; Shen, B.; and Qian, S.-B. (2012) Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution. Proc. Natl. Acad. Sci. USA 109:14728-14729 (E2424-E2432). [PubMed]

Huang, S.-X.; Feng, Z.; Wang, L.; Galm, U.; Wendt-Pienkowski, E.; Yang, D.; Tao, M.; Coughlin, J. M.; Duan, Y.; Shen, B. (2012) A designer bleomycin with significantly improved DNA cleavage activity.  J. Am. Chem. Soc. 134:13501-13509. [PubMed]

Stern-Ginossar, N.; Weisburd, B.; Michalski, A.; Le, V. T. K.; Hein, M. Y.; Huang, S.-X.; Ma, M.; Shen, B.; Qian, S.-B.; Hengel, H.; Mann, M.; Ingolia, N. T.; Weissman, J. S. (2012) Decoding human cytomegalovirus.  Science 338:1088-1093.

Smanski, M. J.; Casper, J.; Peterson, R. M.; Yu, Z.; Rajski, S. R.; Shen B. (2012) Heterologous expression of the platencin gene cluster yields several platencin congeners.  J. Nat. Prod. 75:2158-2167. [PubMed]

Tebbets, B.; Yu, Z.; Stewart, D.; Zhao, L.-X.; Jiang, Y.; Xu, L.-H.; Shen, B.; Klein, B. (2012) Identification of macrotetrolides as antifungal natural products via yeast bioassay: insights into drug spectrum, potency and mode of action.  Medical Mycology, Epub on Aug. 28, 2012 (doi: 10.3109/13693786.2012.710917). [PubMed]

Van Lanen, S.G.; Shen, B. (2012) “Combinatorial Biosynthesis of Anticancer Natural Products” in Antitumor Agents from Natural Products, 2^nd Ed., Kingston, D.G.I.; Cragg, G.M.; Newman, D.J. eds., CRC Press Boca Raton, pp667-694.

Smanski, M. J.; Qu, X.; Liu, W., Shen, B. (2012) “Biosynthesis of pharmaceutical natural products and their pathway engineering”  Chapter 5 In “Organic Chemistry – Breakthroughs and Perspectives”, Ding, K.; Dai, L. eds., Wiley VCH, pp125-180.

Huang, S.-X.; Wang, X.-J.;  Yan, Y.; Wang, J.-D.; Zhang, J.; Liu, C.-X.; Xiang, W.-S.; Shen, B. (2012) Neaumycin - a new macrolide from Streptomyces sp. NEAU-x211. Org Lett. 14:1254-1257. [Pubmed]

Yang, D.; Li, W.; Huang, S.-X.; Shen, B. (2012) Functional characterization of ttnI completing the tailoring steps for tautomycetin biosynthesis in Streptomyces griseochromogenes. Org. Lett. 14:1302-1305. [PubMed]

Lin, S.; Huang, T.; Horsman, G. P.; Huang, S.-X.; Guo, X.; Shen, B. (2012) Specificity of the ester bond-forming condensation enzyme SgcC5 in C-1027 biosynthesis. Org. Lett. 14:2300-2303.[PubMed]

Shen, B.; Thorson, J. S. (2012) Expanding nature's chemical repertoire through metabolic engineering and biocatalysis. Curr. Opinion Chem. Biol. 16:99-100. [PubMed]

Smanski, M. J.; Peterson, R. M.; Huang, S.-X.; Shen, B. (2012) Bacterial diterpene synthases: new opportunities for mechanistic enzymology and engineered biosynthesis. Curr. Opinion Chem. Biol. 16:132-141. [PubMed]

Smanski, M. J.; Peterson, R. M.;  Shen, B. (2012) Platensimycin and platencin biosynthesis in Streptomyces platensis showcasing discovery and characterization of novel diterpene synthases. Methods Enzymol. 515:163-186. [PubMed]

Lohman, J. R.;  Shen, B. (2012) 4-Methylideneimidazole-5-one-containing aminomutases in enediyne biosynthesis. Methods Enzymol. 516:299-319. [PubMed]

Lin, S.; Huang, T.; Shen, B. (2012) Tailoring enzymes acting on carrier protein-tethered substrates in natural product biosynthesis. Methods Enzymol. 516:321-343. [PubMed]

Publications 2011

Huang, Y.; Huang, S.-X.; Ju, J.; Tang, G.; Liu, T.; Shen, B. (2011) Functional characterization of the lnmKLM genes unveiling key intermediates for β-alkylation in leinamycin biosynthesis. Org. Lett. 13:498-501.[PubMed]

Shen, B. (2011) Editorial for the special issue of The Journal of Antibiotics dedicated to the late Professor C Richard Hutchinson. J. Antibiot. 64:3-5.

Yu, Z.; Zhao, L.-X.; Jiang, C.-L.; Duan, Y.; Wong, L.; Carver, K. C.; Schuler, L. A.; Shen, B. (2011) Bafilomycins produced by an endophytic actinomycete Streptomyces sp. YIM56209. J. Antibiot. 64:159-162.[PubMed]

Huang, S.-X.; Yu, Z.; Robert, F., Zhao, L.-X.; Jiang, Y.; Duan, Y.; Pelletier, J., Shen, B. (2011) Cycloheximide and congeners as inhibitors of eukaryotic protein synthesis from endophytic actinomycetes Streptomyces sps. YIM56132 and YIM56141. J. Antibiot. 64:164-166.[PubMed]

Liu, S.; Yu, Z.; Yu, X.; Huang, S.-X., Luo, Y.; Wu, L.; Yang, Z.; Wang, L.; Gunawan, A.; Chan, R.J.; Shen, B.; Zhang, Z.-Y. (2011) SHP2 is a target of the immunosuppressant tautomycetin. Chem. Biol. 18:101-110 [PudMed]

Zhang, Q.; Li, Y.; Chen, D.; Yu, Y.; Duan, L.; Shen, B.; Liu, W. (2011) Radical-mediated enzymatic carbon chain fragmentation-recombination. Nat. Chem. Biol. 7:154-160.[PubMed]

Yang, D.; Zhu, X.; Wu, X.; Feng, Z.; Huang, L.; Shen, B.; Zhang, Y. (2011) Titer improvement of iso-migrastatin in the selected heterologous Streptomyces hosts and related analysis of mRNA expression by quantitative RT-PCR. Appl. Microbiol. Biotechnol. 89:1709-1719.[PubMed]

Galm, U.; Wendt-Pienkowski, E.; Wang, L.; Huang, S.; Unsin, C.; Tao, M.; Coughlin, J. M.; Shen, B. (2011) Comparative analysis of the biosynthetic Gene cluster and pathways for three structurall related antitumor antibiotics bleomycin, tallysomycin and zorbamycin. J. Nat. Prod. 74:526-536.[PubMed]

Chen, Y.; Yin, M.; Horsman, G. P.; Shen, B. (2011) Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus. J. Nat. Prod. 74:420-424.[PubMed]

Yu, Z.; Vodanovic-Jankovic, S.; Ledeboer, N.; Huang, S.-X.; Rajski, S. R.; Kron, M.; Shen, B. (2011) Tirandamycins from Streptomyces sp. 17944 Inhibiting the Parasite Brugia malayi Asparagin tRNA Synthetase. Org. Lett. 13:2034-2037.[PubMed]

Yin, M.; Lu, T.; Zhao, L.-X.; Chen, Y.; Huang, S.-X.; Lohman, J. R.; Xu, L.-H.; Jiang, C.-L.; Shen, B. (2011) The missing C-17 O-methyltransferase in geldanamycin biosynthesis. Org. Lett. 13:3726-3729.[PubMed]

Smanski, M. J.; Yu, Z.; Casper, J.; Lin, S.; Peterson, R. M.; Chen, Y.; Wendt-Pienkowski, E.; Rajski, S. R.; Shen, B. (2011) Dedicated ent-Kaurene and ent-Atiserene Synthases for Platensimycin and Platencin Biosynthesis. Proc. Natl. Acad. Sci. USA 108:13498-13503.[PubMed]

Zhao, L.-X.; Huang, S.-X.; Tang, S.-K.; Jiang, C.-L.; Duan, Y.; Beutler, J. A.; Henrich, C. J.; McMahon, J. B.; Schmid, T.; Blees, J. S.; Colburn, N. H.; Rajski, S. R.; Shen, B. (2001) Actinopolysporins A-C and tubercidin as a Pdcd4 stabilizer from the halophilic actinomycete Actinopolyspora erythraea YIM 90600. J. Nat. Prod. 74:1990-1995.[PubMed]

Publications 2010

Tao, M.; Wang, L.; Wendt-Pienkowski, E.; Zhang, N.; Yang, D.; Galm, U.; Coughlin, J. M.; Xu, Z.; Shen, B. (2010) Functional characterization of tlmH in Streptoalloteichus hindustanus E465-94 ATCC31158 unveiling new insight into tallysomycin biosynthesis and affording a new bleomycin analog. Mol. BioSyst. 6:349-356. [PubMed]

Schneider-Poetsch, T.; Ju, J.; Eyler, D. E.; Dang, Y.; Bhat, S.; Merrick, W. C.; Green, R.; Shen, B.; Liu, J. O. (2010) Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nat. Chem. Biol. 6:209-217. [PubMed]

Chen, Y.; Smanski, M. J.; Shen, B. (2010) Improvement of secondary metabolite production in Streptomyces by manipulating pathway regulation. Appl. Microbiol. Biotechnol. 86:19-25. [PubMed]

Zhang, N.; Zhu, X.; Yang, D.; Cai, J.; Tao, M.; Wang, L.; Duan, Y.; Shen, B.; Xu, Z. (2010) Improved production of tallysomycin H-1 in Streptomyces hindustanus SB8005 strain by fermentation optimization. Appl. Microbiol. Biotechnol. 86:1345-1353. [PubMed]

Yu, Z.; Smanski, M. J.; Peterson, R. M.; Marchillo, K.; Andes, D.; Rajski, S. R.; Shen, B. (2010) Engineering of Streptomyces platensis MA7339 for overproduction of platencin and congeners. Org. Lett. 12:1744-1747. [PubMed]

Luo, Y.; Li, W.; Ju, J.; Yuan Q.; Peters, N. R.; Hoffmann, F. M.; Huang, S.; Bugni, T.; Rajski, S. R.; Osada, H.; Shen, B. (2010) Functional characterization of TtnD and TtnF unveiling new insights into tautomycetin biosynthesis. J. Am. Soc. Chem. 132:6663-6671. [PubMed]

Zhao, C.; Coughlin, J. M.; Ju, J.; Zhu, D.; Wendt-Pienkowski, E.; Zhou, X.; Wang, Z.; Shen, B.; Deng, Z. (2010) Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an “acyltransferase-less” polyketide synthase that incorporates two distinct extender units. J. Biol. Chem. 285:20097-20108. [PubMed]

Horsman, G. P.; Chen, Y.; Thorson, J. S.; Shen, B. (2010)  Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes. Proc. Natl. Acad. Sci. U.S.A. 107:11331-11335.

Chen, Y.; Yin, M.; Horsman, G. P.; Huang, S.;  Shen, B. (2010)  Manipulation of pathway regulation in Streptomyces globisporus for overproducing the enediyne antitumor antibiotic C-1027. J. Antibiot. 63:482-485. [PubMed]

Huang, S.-X.; Powell, E.; Rajski, S.R.; Zhao, L.; Jiang, C.; Duan, Y.; Xu, W.; Shen, B.  (2010) Discovery and total Synthesis of a new estrogen receptor heterodimering actinopolymorphol A from Actinopolymorpha rutilus.  Org. Lett. 12:3525-3527. [PubMed]

Powell, E.; Huang, S.-X.; Xu, Y.; Rajski, S.R.; Wang, Y.; Peters, N.; Guo, S.; Xu, H.E.; Hoffmann, F.M.; Shen, B.; Xu, W. (2010) Identification and characterization of a novel estrogenic ligand actinopolymorphol A. Biochem. Pharmacol. 80:1221-1229. [PubMed]

Ling, J.; Horsman, G. P.; Huang, S.-X.; Luo, Y.; Lin, S.; Shen, B. (2010) Enediyne antitumor antibiotic maduropeptin biosynthesis featuring a C-methyltransferase that acts on a CoA-tethered aromatic substrate. J. Am. Chem. Sc. 132:12534-12536. [PubMed]

Lin, S.; Horsman, G.P.; Shen, B. (2010) Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster. Org. Lett. 12:3816-3819. [PubMed]

Rajski, S. R.; Shen, B. (2010) Multifaceted modes of action for the glutarimide-containing polyketides revealed. ChemBioChem 11:1951-1954. [PubMed]

Wu, X.; Yang, D.; Zhu, X.; Feng, Z.; Lv, Z.; Zhang, Y.; Shen, B.; Xu, Z. (2010) iso-Migrastatin titer improvement in the engineered Streptomyces lividans SB11002 strain by optimization of fermentation conditions. Biotechnol Bioprocess Eng. 15:664-669. [PubMed]

Yu, Y.; Guo, H.; Zhang, Q.; Duan, L., Ding, Y.; Lei, C.; Shen, B.; Liu, W. (2010) NosA catalyzing carboxyl-terminal amide formation in nosiheptide maturation via an enamine dealkylation on the serine-extended precursor peptide. J. Am. Chem. Soc. 132:16324-16326. [PubMed]

Chen, Y.; Wendt-Pienkowski, E.; Ju, J.; Rajski, S.R.; Shen, B. (2010) Characterization of FdmV as an amide synthetase for fredericamycin A biosynthesis in Streptomyces griseus ATCC43944. J. Biol. Chem. 285:38853-38860. [PubMed]

2009 Publications

Galm, U.; Wendt-Pienkowski, E.; Wang, L.; George, N.P.; Oh, T.-J.; Yi, F.; Tao, M.; Coughlin, J.M; Shen, B. (2009) The biosynthetic gene cluster of zorbamycin, a member of the bleomycin family of antitumor antibiotics, from Streptomyces flavoviridis ATCC21892. Mol. BioSyst. 4:77-90. [PubMed]

Beerman, T.A.; Gawron, L.S.; Shin, S.; Shen, B.; McHugh, M.M. (2009) C-1027, a radiomimetic enediyne anticancer drug, preferentially target hypoxic cells. Cancer Res. 69:593-598. [PubMed]

Feng, Z,; Wang, L.; Rajski, S.R.; Xu, Z.; Coeffet-LeGal, M,F.; Shen, B. (2009) Engineered production of iso-migrastatin in heterologous Streptomyces hosts. Bioorg. Med. Chem. 17:2147-2159. [PubMed]

Ju, J.; Rajski, S.R.; Lim, S.-K.; Seo, J.-W.; Peters, N.R.; Hoffmann, F.M.; Shen, B. (2009) Lactimidomycin, iso-migrastatin and related glutarimide-containing 12-membered macrolides are extremely potent inhibitors of cell migration. J. Am. Chem. Soc. 131:1370-1371. [PubMed]

Lin, S.; Van Lanen, S.G.; Shen, B. (2009) A free-standing condensation enzyme catalyzing ester bond formation in C-1027 biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 106:4183-4188. [PubMed]

Horsman, G.P.; Van Lanen, S.G.; Shen, B. (2009)  Iterative type I polyketide synthase for enediyne core biosynthesis. Methods in Enzymology 459:97-112. [PubMed]

Jiang, H.; Rajski, S. R.; Shen, B. (2009) Tandem acyl carrier protein domains in polyunsaturated fatty acid synthases. Methods in Enzymology 459:79-96. [PubMed]

Cheng, Y.-Q.; Coughlin, J.M.; Lim, S.-K.; Shen, B. (2009) Type I polyketide synthases that require discrete acyltransferases. Methods in Enzymology 459:165-186. [PubMed]

Smanski, M.J.; Peterson, R.M.; Rajski, S.R.; Shen, B. (2009) Engineered Streptomyces platensis strains that overproduce antibiotics platensimycin and platencin. Antimicrob. Agents Chermother. 53:1299-1304. [PubMed]

Li, W.; Luo, Y.; Ju, J.; Rajski, S.R. ; Osada, H. ; Shen, B. (2009) Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. J. Nat. Prod. 72:450-459. [PubMed]

Liao, R.; Duan, L.; Lei, C.; Pan, H.; Ding, Y.; Zhang, Q.; Chen, D.; Shen, B.; Yu, Y.; Liu, W. (2009) Thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications. Chem. Biol. 16:141-147. [PubMed]

Wang, L.; Tao, M.; Wendt-Pienkowski, E.; Galm, U.; Coughlin, J.M.; Shen, B. (2009) Functional characterization of tlmK unveiling unstable carbinolamide intermediates in the tallysomycin biosynthetic pathway. J. Biol. Chem. 284:8256-8264. [PubMed]

Huang, S.-X.; Zhao, L.-X.; Tang, S.-K.; Jiang, C.-L.; Duan, Y.; Shen, B. (2009) Erythronolides H and I, new erythromycin congeners from a halophilic actinomycete Actinopolyspora sp. YIM90600. Org. Lett. 11:1353-1356. [PubMed]

Ju, J.; Li, W.; Yuan, Q.; Peters, N. ; Hoffmann, F.M., Rajski, S.R.; Osada, H.; Shen, B. (2009) Functional characterization of ttmM unveils new tautomycin analogs and insight into tautomycin biosynthesis and activity. Org. Lett. 11:1639-1642. [PubMed]

Adler, J.; Cook, M.; Luo, Y.; Pitt, S.; Ju, J.; Li, W.; Shen, B.; Kunnimalaiyaan, M.; Chen, H. (2009) Tautomycetin and tautomycin suppress the growth of medullary thyroid cancer cells via inhibition of GSK-3beta. Mol. Cancer Therap. 8:914-920. [PubMed]

Liu, T.; Huang, Y.; Shen, B. (2009) The bifunctional acyltransferase/decarboxylase LnmK as the missing link for b-alkylation in polyketide biosynthesis. J. Am. Chem. Soc. 131:6900-6901. [PubMed]

Pinchot, S. N.; Adler, J. T.; Luo, Y.; Ju, J.; Li, W.; Shen, B.; Kunnimalaiyaan, M.; Chen, H. (2009) Tautomycin suppress the growth and neuroendocrine hormone markers in carcinoid cells through activation of the raf-1 pathway. Am. J. Surg. 197:313-319. [PubMed]

Chen, Y.; Wendt-Pienkowski, E.; Rajski, S. R.; Shen, B. (2009) In vivo investigation of the roles of FdmM and FdmM1 in fredericamycin biosynthesis unveiling a new family of oxygenases. J. Biol. Chem. 284:24735-24743. [PubMed]

Yu, Y.; Duan, L.; Zhang, Q.; Liao, R.; Ding, Y.; Pan, H.; Wendt-Pienkowski, E.; Tang, G.; Shen, B.; Liu, W. (2009) Nosiheptide biosynthesis featuring a unique indole side ring formation on the characteristic thiopeptide framework. ACS Chem. Biol. 4:855-864. [PubMed]

Van Lanen, S. G.; Lin, S.; Horsman, G. P.; Shen, B. (2009) Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027. FEMS Microbiol Lett. 300:237-241. [PubMed]

Lim, S.-K.; Ju, J.; Zazopoulos, E.; Jiang, H.; Seo, J.-W.; Chen, Y.; Feng, Z.; Rajski, S. R.; Farnet, C. M.; Shen, B. (2009) iso-Migrastatin, migrastatin, and dorrigocin production in Streptomyces platensis NRRL18993 is governed by a single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase. J. Biol. Chem. 284:29746-29756. [PubMed]

Cooke, H. A.; Guenther, E. L.; Luo, Y.; Shen, B.; Bruner, S. G. (2009) Molecular basis of substrate promiscuity for the SAM-dependent NcsB1 O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin� Biochemistry 48:9590-9598. [PubMed]

Lin, S.; Horsman, G. P.; Chen, Y.; Li, W.; Shen, B. (2009) Characterization of the SgcF epoxide hydrolase supporting a (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis. J. Am. Chem. Soc. 131:16410-16417. [PubMed]

Rajski, S.R.; Shen, B. (2009) The role of evolution in the discovery of new drugs and chemicals. In ACS Symposium Series 1025: Chemical Evolution II: From Origins of Life to Modern Society, Zaikowski, L.; Friedrich, J.M.; Seidel, S.R. Eds., ACS, Washington DC, pp205-232.

Hutchinson, C.R.; Shen, B. (2009) Pharmacognosy in Action US Academia: University of Wisconsin-Madison. In The American Society of Pharmacognosy 50 Years of Progress in Natural Products Research 1959-2009. Cragg, G.M.; Beutler, J.A.; Jones, W.P. eds., The American Society of Pharmacognosy, Omnipress, Madison, Wisconsin, 2009, pp140-142.

Shen, B. (2009) Expanding Horizons: Combinatorial Biosynthesis. In The American Society of Pharmacognosy 50 Years of Progress in Natural Products Research 1959-2009. Cragg, G.M.; Beutler, J.A.; Jones, W.P. eds., The American Society of Pharmacognosy, Omnipress, Madison, Wisconsin, 2009, pp248-250.

2008 Publications

Van Lanen, S. G.; Lin, S.; Shen, B. (2008) The biosynthesis of the antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. Proc. Natl. Acad. Sci. U.S.A. 105:494-499. (Featured in C & E News, January 14, 2008, p36) [PubMed]

Van Lanen, S. G.; Shen, B. (2008) Advances in polyketide synthase structure and mechanism. Curr. Opinion Drug Discov. Develop. 11:186-195.

Zhang, J.; Van Lanen, S. G.; Ju, J.; Liu, W.; Dorrestein, P. C.; Li, W.; Kelleher, N. L.; Shen, B. (2008) A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 105:1460-1465. [PubMed]

 Chen, Y.; Luo, Y.; Ju.; Wendt-Pienkowski, E.; Rajski, S. R.; Shen, B. (2008) Identification of fredericamycin E from Streptomyces griseus: insights into fredericamycin A biosynthesis highlighting carbaspirocycle formation.  J. Nat. Prod. 71:431-437. [PubMed]

 Song, D.; Coughlin, J.; Ju, J.; Zhou, X.; Shen, B.; Zhao, C.; Deng, Z. (2008) Alternative method for site-directed mutagenesis of complex polyketide synthase in Streptomyces albus JA3453. Acta Biochim. Biophys. Sinica 40:319-326. [PubMed]

 Montavon, T. J.; Christianson, C. V.; Festin, G. M.; Shen, B.; Bruner, S. D. (2008) Design and characterization of mechanism-based inhibitors for the tyrosine aminomutase SgTAM. Bioorg. Med. Chem. Lett. 18:3099-3102. [PubMed]

 Lin, S.; Van Lanen, S. G.; Shen, B. (2008) Characterization of the two-component, FAD-dependent monooxygenase SgcC that requires carrier protein-tethered substrates for the biosynthesis of the enediyne antitumor antibiotic C-1027.  J. Am. Chem. Soc. 130:6616-6623. [PubMed]

 Jiang, H.; Zirkle, R.; Metz, J. G.; Braun, L.; Richter, L.; Van Lanen, S. G.; Shen, B. (2008) The role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis.  J. Am. Chem. Soc. 130:6336-6337.  (Featured in Nat. Chem. Biol. 2008, 4:340) [PubMed]

 Luo, Y.; Lin, S.; Zhang, J.; Cooke, H. A.; Bruner, S. D.; Shen, B. (2008)  Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin. J. Biol. Chem. 283:14694-14702. [PubMed]

Van Lanen, S. G.; Shen, B. (2008) Biosynthesis of enediyne antitumor antibiotics. Curr. Topics Med. Chem. 8:448-459. [PubMed]

 Chen, Y.; Wendt-Pienkowski, E.; Shen, B. (2008) Identification and utilities of FdmR1 as a SARP activator for fredericamycin production in Streptomyces griseus ATCC49344 and heterologous hosts.  J. Bcateriol. 190:5587-5596. [PubMed]

 Li, W.; Ju, J.; Rajski, S. R. ; Osada, H. ; Shen, B. (2008) Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. J. Biol. Chem. 283:28607-28617.

 Galm, U.; Wang, L.; Wendt-Pienkowski, E.; Tao, M.; Coughlin, J. M.; Shen, B. (2008) In vivo manipulation of the bleomycin biosynthetic gene cluster in Streptomyces verticillus ATCC15003 revealing new insights into its biosynthetic pathway. J. Biol. Chem. 283:28236-28245.

 Ju, J.; Rajski, S. R.; Lim, S.-K. ; Seo, J.-W. ; Peters, N. ; Hoffmann, F. M.; Shen, B. (2008) Evaluation of new migrastatin and dorrigocin congeners unveils cell migration inhibitors with dramatically improved potency. Bioorg. Med. Chem. Lett. 18:5951-5954.

2007 Publications

Tao, M.; Wang, L.; Wendt-Pienkowski, E.; George, N. P.; Galm, U.; Zhang, G.; Coughlin, J. M.; Shen, B. (2007) The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insights into the biosynthesis of the bleomycin family of antitumor antibiotics. Molecular BioSystems, 3:60-74. [Pubmed]

Shen, B.; Cheng, Y.-Q.; Christenson, S. D.; Jiang, H.; Ju, J.; Kwon, H.-J.; Lim, S.-K.; Liu, W.; Nonaka, K.; Seo, J.-W.; Smith, W. C.; Standage, S.; Tang, G.-L.; Van Lanen, S.; Zhang, J. (2007) Polyketide biosynthesis beyond the type I, II, and III polyketide synthase paradigms: a progress report. In ACS symposium series 955, Polyketides: Biosynthesis, Biological Activities, and Genetic Engineering.  Rimando, A.M.; Baerson, S.R. eds., ACS, pp154-166.

Kennedy, D. R.; Gawron, L. S.; Ju, J.; Liu, W.; Shen, B.; Beerman, T. A.  (2007) Single chemical modifications of the enediyne core of C-1027, a radiomimetic antitumor drug, result in markedly varied cellular responses to DNA double strand breaks. Cancer Res, 67: 773-781.[Pubmed]

Wang, L.; Yun, B.-S.; George, N. P.; Wendt-Pienkowski, E.; Galm, U.; Oh, T.-J.; Coughlin, J. M.; Zhang, G.; Tao, M.; Shen, B. (2007) The Glycopeptide Antitumor Antibiotic Zorbamycin from Streptomyces flavoviridis ATCC 21892: Strain Improvement and Structure Elucidation. J. Nat. Prod. 70: 402-406. [Pubmed]

Guan, C.; Ju, J.;  Borlee, B. R.; Williamson, L. L.; Shen, B.; Raffa, K. F.; Handelsman, J. (2007)  Signal Mimics Derived from a Metagenomic Analysis of Gypsy Moth Gut Microbiota. Appl. Environ. Microbiol. 73:3669-3676. [AEM]

Carl V. Christianson, Timothy J. Montavon, Steven G. Van Lanen, Ben Shen, and Steven D. Bruner (2007) The structure of L-tyrosine 2,3-aminomutase from C-1027 enediyne antitumor antibiotic biosynthetic pathway.  Biochemistry 46:7025-7214. [Pubmed]

Cooke, H. A.; Zhang, J.; Griffin, M. A.; Nonaka, K.; Van Lanen, S. G.; Shen, B.; Bruner, S. D. (2007) Characterization of NcsB2 as a Promiscuous Naphthoic Acid/Coenzyme A Ligase Integral to the Biosynthesis of the Enediyne Antitumor Antibiotic Neocarzinostatin. J. Am. Chem. Soc. 129:7728-7729. [ACS]

Tang, G.-L.; Cheng, Y.-Q.; Shen, B. (2007) Chain initiation in the leinamycin-producing hybrid non-ribosomal peptide/polyketide synthase from Streptomyces atroolivaceus S-140: discrete, monofunctional adenylation enzyme and peptidyl carrier protein that directly load (D)-alanine. J. Biol. Chem. 282:20273-20282. [JBC]

Lin, S.; Van Lanen, S.G.; Shen, B. (2007) Regiospecific chlorination of (S)-β-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027. J. Am. Chem. Soc. 129:12432-12438.[ACS]

Kennedy, D. R. ; Ju J.; Shen, B.; Beerman, T. A. (2007) Single modifications of an enediyne chromophore can confer an ability to induce DNA double strand breaks, interstrand crosslinks or both with concominant changes in PIKK regulation of DNA damage response. Proc. Natl. Acad. Sci. U.S.A. 104:17632-17637. [PNAS]

Van Lanen, S.G.; Oh T.-J.; Liu, W.; Wendt-Pienkowski, E.; Shen, B. (2007) Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. J. Am. Chem. Soc. 129:13082-13094. [ACS]

Galm, U.; Shen, B. (2007) Natural product drug discovery: the times have never been better. Chem. Biol. 14:1098-1104. [Chem. Biol.]

Ju, J.; Seo, J.-W.; Her, Y.; Lim, S.-K.; Shen, B. (2007) New Lactimidomycin Congeners Shed Insight into Lactimidomycin Biosynthesis in Streptomyces amphibiosporus. Org. Lett. 9:5183-5186. [ACS]
Christianson, C.V.; Montavon, T.J.; Festin, G.M.; Cooke, H.A.; Shen, B.; Bruner, S.D. (2007) The mechanism of MIO-based aminomutase chemistry in β-tyrosine biosynthesis. J. Am. Chem. Soc. 129:15744-15745.

2006 Publications

Tang, G.-L.; Cheng, Y.-Q.; Shen, B. (2006) Polyketide chain skipping mechanism in the biosynthesis of the hybrid nonribosomal peptide-polyketide antitumor antibiotic leinamycin in Streptomyces atroolivaceus S-140. J. Nat. Prod. 69:387-393.

Van Lanen, S.; Shen, B. (2006) Microbial genomics for the improvement of natural product discovery.  Curr. Opinion Microbiol. 9:252-262.

Zhao, C.; Ju, J.; Christenson, S. D.; Smith, W. C.; Song, D.; Zhou, X.; Shen, B.; Deng, Z. (2006) Utilization of the methoxymalony-acyl carrier protein biosynthetic locus for the cloning of the oxazolomycin biosynthetic gene cluster from Streptomyces albus.  J. Bacteriol. 198:4141-4147.

Li, W.; Ju, J.; Osada, H.; Shen, B. (2006) Utilization of the methoxymalony-acyl carrier protein biosynthetic locus for the cloning of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillus.  J. Bacteriol. 198:4148-4152.

Huang, Y.; Wendt-Pienkowski, E.; Shen, B. (2006) A dedicated phosphopantetheinyl transferase for the fredericamycin polyketide synthase from Streptomyces griseus. J Biol. Chem. 281, 29660-29668.

Van Lanen S.G.; Lin S.; Dorrestein, P.C.; Kelleher, N.L.; Shen B. (2006) Substrate specificity of the adenylation enzyme SgcC1 involved in the biosynthesis of the enediyne antitumor antibiotic C-1027. J. Biol. Chem. 281, 29633-29640.

Van Lanen, S.; Shen, B. (2006) Biosynthesis of enediyne antitumor antibiotics. (2006) Drug Discovery Today: Technol. 3: 2006, 3, 285-292.
Dorrestein, P. C.; Van Lanen, S. G.; Li, W.; Zhao, C.; Deng, Z.; Shen, B.; Kelleher, N. L. (2006) The bifunctional glyceryl transferase/phosphatase OzmB beloning to the HAD superfamily that diverts 1,3-biophosphoglycerate into polyketide biosynthesis.  J. Am. Chem. Soc. 128, 10386-10387.
Galm, U.; Shen, B. (2006) Expression of biosynthetic gene clusters in heterologous hosts for natural product production and combinatorial biosynthesis.  Exp. Opin. Drug Discov. 1:409-437.

Ju, J.; Lim, S.-K.; Jiang, H.; Seo, J.-W.; Her, H. Shen, B. (2006) Thermolysis of iso-migrastatin and its congeners via [3,3]-sigmatropic rearrangement: a new route to the synthesis of migrastatin and its analogs. Org. Lett. 8: 5865

2005 Publications

Ju, J.; Lin, S-K.; Jiang, H.; Seo, J.-Woo S., Shen, B. (2005)  Iso-Migrastatin congeners from Streptomyces platensis and generation of a glutarimide polyketide library featuring the dorrigocin, lactimidomycin, migrastatin, and NK30424 scaffolds. J. Am. Chem. Soc. 127, 11930-11931. [ PubMed ]

 Van Lanen, S.; Dorrestein, P. C.; Christenson, S. D.; Liu, W.; Ju, J.; Kelleher, N. L.; Shen, B. (2005) Biosynthesis of the β-amino acid  moiety of the enediyne antitumor antibiotic C-1027 featuring β-aminoacyl-S-carrier protein intermediates. J. Am. Chem. Soc.  127, 11594-11595. [ PubMed ] 

Wendt-Pienkowski, E.; Huang, Y.; Zhang, J.; Li, B.; Jiang, H.; Kwon,  H.-J.; Hutchinson, C.R.; Shen, B. (2005) Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. J. Am. Chem. Soc. 127, 16442-16452.[ PubMed ]

Shen, B.; Chen, M.; Cheng, Y.-Q.; Du, L.; Edwards, D.J.; George, N.P.; Huang, Y.; Oh, T.; Sanchez, C.; Tang, G.-L.; Wendt-Pienkowski, E.; Yi, F. (2005) Prerequisites for Combinatorial Biosynthesis: Evolution of Hybrid NRPS-PKS Gene Clusters in Biocombinatorial Approaches for Drug Finding, in Ernst Schering Research Foundation Workshop, Springer-Verlag, Berlin, 2005, 51:107-126.

Thomas, M.G.; Bixby, K.A.; Shen, B. (2005) ¨Combinatorial Biosynthesis of Antitcancer Natural Products〃 in Antitumor Agents from Natural Products, Kingston, D.G.I.; Cragg, G.M.; Newman, D.J. eds., CRC Press, Boca Raton,  2005, 519-551.

Galm, U.; Hager, M.H.; Van Lanen, S.G.; Ju, H.; Thorson, J.S.; Shen, B. (2005) Antitumor Antibiotics: Bleomycin, Enediynes, and Mitomycin. Chem. Rev. 105:739-758. [ PubMed ]

Shen, B.; Liu, W.; Nonaka, K. (2005) ¨Enediyne Natural Products: Biosynthesis and Prospect towards Engineering Novel Antitumor Agents〃 in Frontiers in Medicinal Chemistry, Atta-ur-Rahman Ed., Bentham Science Publishers, 2005, Vol. 2, pp357-369.

Liu, W.; Nonaka, K.; Nie, P.; Zhang, J.; Christenson, S.D.; Bae, J.; Van Lanen, S.G.; Zazopoulos, E.; Farnet, C.M.; Yang, C.F.; Shen, B. (2005) The Neocarzinostatin Biosynthetic Gene Cluster from Streptomyces carzinostaticus ATCC15944 involving two iterative type I polyketide synthase. Chem. Biol. 12: 293-302. [ PubMed ]

Ju, J.; Lim, S.-K.; Jiang, H.; Shen, B. (2004) Migrastatin and Dorrigocins Are Shunt Metabolites of iso-Migrastatin. J. Am. Chem. Soc. 127:1622-1623. [ PubMed ]

2004 Publications

Ju, J.; Ozanick, S. G.; Shen, B.; Thomas, M. G. (2004) Conversion of (2S)-Arginie to (2S, 3R)-Capreomycidine by VioC and VioD from the Viomycin Biosynthetic Pathway of Streptomyces sp ATCC11861. ChemBioChem, 5:1281-1285. [ PubMed ]

Shen, B. (2004) Accessing natural products by combinatorial biosynthesis, Sci. STKE, Vol. 2004, Issue 225, pp. pe14. [ PubMed ]

Murrell, J. M.; Liu, W.; Shen, B. (2004) Cloning and characterization of the SgcA1 a-D-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor antibiotic C-1027 biosynthetic pathway and overexpression of sgcA1 in Streptomyces globisporus to improve C-1027 production, J. Nat. Prod. 67: 206-213. [ PubMed ]

Tang, G.-L.; Cheng, Y.-Q.; Shen, B. (2004) The Biosynthetic Gene Cluster of the Antitumor Antibiotic Leinamycin from Streptomyces atroolivaceus S-140 Revealing Unprecedented Architectural Complexity for a Hybrid Nonribosomal Peptide Synthetase-Polyketide Synthase.  Chem. Biol., 11: 33-45. [ PubMed ]

2003 Publications

Liu, W.; Ahlert, J.; Gao, Q.; Wendt-Pienkowski, E.; Shen, B.; Thorson, J.S. (2003) Rapid PCR Ampilification of Minimal Enediyne PKS Cassette Leads to a Predictive Familial Classification Model for Divergent Enediyne PKS Genes.  Proc. Natl. Acad. Sci. USA, 100:11959-11963.  [ PubMed ]

Christenson, S.D.; Wu, W.; Shen, B.; Toney, M.D. (2003) Kinetic Analysis of the 4-Methylideneimidazole-5-one-Containing Tyrosine Aminomutase in Enediyne Antitumor Antibiotic C-1027 Biosynthesis. Biochemistry, 42: 12708-12718.  [ PubMed ]

Schneider, T.L.; Shen, B.; Walsh, C.T. (2003) Oxidase domains in epothilone and bleomycin biosynthesis: thiazoline to thiazole oxidation during chain elongation. Biochemistry, 42: 9722-9730.[ PubMed ]

Du, L.; Chen, M.; Zhang, Y.; Shen, B. (2003) The BlmIII and BlmIV nonribosomal peptide Synthetase-catalyzed biosynthesis of the bleomycin bithiazole moiety involving both in cis and in trans aminoacylation. Biochemistry, 42: 9731-9740. [ PubMed ]

Shen, B.; Liu, W.; Nonaka, K. (2003) Enediyne Natural Products: Biosynthesis and Prospect towards Engineering Novel Antitumor Agents.  Curr. Med. Chem. 10: 2317-2325.  [ PubMed ]

Christenson, S.D.; Liu, W.; Toney, M.D.; Shen, B. (2003) A novel dehydroalanine-dependent tyrosine aminomutase in C-1027 enediyne biosynthesis.   J. Am. Chem. Soc. 125: 6062-6063.[ PubMed ]

Du, L.; Cheng, Y.Q.; Ingenhorst, G.; Tang, G.-L.; Huang, Y.; Shen, B. (2003) Hybrid peptide-polyketide natural products: biosynthesis and prospects towards engineering novel molecules” in Genetic Engineering, Principle and Methods, Setlow, J. K. ed., Kluwer Academic/Plenum Publishers, vol 25, 227-267.

Shen, B. (2003) Polyketide biosynthesis beyond the type I, II, and III polyketide synthase paradigms.  Curr. Opinion Chem. Biol. 7:285-295.    [ PubMed ]   

Cheng, Y.-Q.; Tang, G.-L.; Shen, B. (2003) Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis. Proc. Natl. Acad. Sci. USA, 100:3149-3154. [ PubMed ]

Zazopoulos, E.; Huang, K.; Staffa, A; Liu, W.; Bachmann, B. O.; Nonaka, K.; Ahlert, J.; Thorson, J. T.; Shen, B.; Farnet, C. M. (2003) A genomics-guided approach for discovering and expressing cryptic metabolic pathways. Nat. Biotechnol. 21: 187-190.[ PubMed ]

2002 Publications

Cheng, Y.-Q.; Tang, G.-L.; Shen, B. (2002) Identification and Localization of the Antitumor macrolactam Leinamycin Biosynthesis Gene Cluster from Streptomyces atroolivaceus S-140. J. Bacteriol., 184 , 7013-7024.[ PubMed ]

Kwon, H.-J.; Smith, W. C.; Scharon, A. J.; Hwang, S. H.; Kurth, M. J.; Shen, B. (2002) C—O Bond Formation by Polyketide Synthases. Science, 297: 1327-1330. [abstract] [Full text].

Liu, W.; Christenson, S. D.; Standage, S.; Shen, B. (2002) Biosynthesis of the Enediyne Antitumor Antibiotic C-1027. Science, 297: 1170-1173.[abstract] [Full text]

Shen, B.; Kwon, H.-J. (2002) Macrotetrolide biosynthesis: a novel type II polyketide synthase, The Chemical Record, 2:389-396. [ PubMed ]

Shen, B.; Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J.; Chen, M.; Murrell, J.M. (2002) Cloning and Characterization of the Bleomycin Biosynthetic Gene Cluster from Streptomyces verticillus ATCC15003. J. Nat. Prod. 65: 422-431. [ PubMed ]

2001 Publications

Rafanan Jr., E.R.; Le, L.; Zhao, L.; Decker, H.; Shen, B. (2001) Cloning, Sequencing, and Heterologous statement of the elmGHIJ Genes Involved in the Biosynthesis of the Polyketide Antibiotic Elloramycin from Streptomyces olivaceus Tü2353. J. Nat. Prod., 64:444-449.  [ PubMed ]

Kwon, H.-J.; Smith, W.C.; Xiang, L.; Shen, B. (2001) Cloning and heterologous statement of the macrotetrolide biosynthetic gene cluster revealed a novel polyketide synthase that lacks an acyl carrier proteine. J. Am. Chem. Soc., 123:3385-3386. [ PubMed ]

Sanchez, C.; Du, L.; Edwards, D.J.; Toney, M.D,; Shen, B. (2001) Cloning and Characterization of a Phosphopantetheinyl Transferase from Streptomyces verticillus ATCC15003, the Producer of the Hybrid Peptide-Polyketide Antitumor Drug Bleomycin. Chem. Biol., 8:725-738. [ PubMed ]

Du, L.; Sanchez, C.; Shen, B. (2001) Biosynthesis of Hybrid Peptide and Polyketide Metabolites: Prospect towards Engineering Novel Molecules. Met. Engineer., 3:78-95. 

Shen, B.; Du, L. (2001) Biosynthesis of hybrid peptide-polyketide natural products. Curr. Opin. Drug Discov. Develop., 4:215-228. 

Shen, B.; Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J. (2001) A Model for Hybrid Peptide-Polyketide Biosynthesis: The Bleomycin Biosynthetic Gene Cluster. J. Ind. Microbiol. Biotechnol., 27:378-385.[ PubMed ]

2000 Publications

Liu, W.; Shen, B. (2000) Genes for Production of the Enediyne Antitumor Antibiotic C-1027 in Streptomyces globisporus Are Clustered with the cagA Gene That Encodes the C-1027 Apoprotein. Antimicrob. Agents Chermother., 44:382-392. [ PubMed ]

Smith, W. C.; Xiang, L.; Shen, B. (2000) Genetic Localization and Molecular Characterization of the nonS Gene Required for Macrotetrolide Biosynthesis in Streptomyces griseus DSM40695. Antimicrob. Agents Chermother., 44:1809-1817. [ PubMed ]

Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J.; Shen, B. (2000) The Biosynthetic Gene Cluster for the Antitumor Drug Bleomycin from Streptomyces verticillus ATCC15003 Supporting Functional Interactions between Nonribosomal Peptide Synthetases and a Polyketide Synthase” Chem. Biol., 7:623-642.  [ PubMed ]

Du, L.; Chen, M.; Sanchez, C.; Shen, B. (2000) An Oxidation Domain in the BlmIII Nonribosomal Peptide Synthetase Probably Catalyzing Thiazole Formation in the Biosynthesis of the Antitumor Drug Bleomycin in Streptomyces verticillus ATCC15003. FEMS Microbiol. Lett., 189:171-175.[ PubMed ]

Rafanan Jr., E.R.; Hutchinson, C.R.; Shen, B. (2000) Triple Hydroxylation of Tetracenomycin A2 to Tetracenomycin C Involving Two Molecules of O2 and One Molecule of H2O. Org. Lett., 2:3225-3227. [ PubMed ]

Beynon, J.D.; Rafanan Jr., E.R.; Shen, B.; Fisher, A.J. (2000) Crystallization and Preliminary X-ray Analysis of Tetracenomycin A2 Oxygenase: A Flavoprotein Hydroxylase Involved in Polyketide Biosynthesis. Acta Cryst., D56:1647-1651. [ PubMed ]

Shen, B. (2000) Aromatic Polyketide Biosynthesis in Topics in Current Chemistry: Biosynthesis Vol. II, Leeper, F. J.; Vederas, J. C. eds, Springer-Verlag:Berlin, 209:1-51.

1999 Publications

Du, L.; Shen, B. (1999) Identification and Characterization of a Type II Peptidyl Carrier Protein from the Bleomycin Producer Streptomyces verticillus ATCC15003. Chem. Biol., 6:507-517. [ PubMed ]

Shen, B.; Du, L.; Sanchez, C.; Chen, M.; Edwards, D.J. (1999) Bleomycin Biosynthesis in Streptomyces verticillus ATCC15003: A Model of Hybrid Peptide and Polyketide Biosynthesis” Bioorg. Chem., 27:155-171. 

Thorson, J.S.; Shen, B.; Whitwam, R.E.; Liu, W.; Li, Y.; Ahlert, J. (1999) Enediyne Biosynthesis and Self-resistance: A Progress Report Bioorg. Chem., 27:172-188. 

1991-1996 Publications

Shen, B.; Summers, R. G.; Gramajo, H.; Bibb, M. J.; Hutchinson, C. R. (1992) Purification and Characterization of the Acyl Carrier Protein of the Streptomyces glaucescens Tetracenomycin C Polyketide Synthase. J. Bacteriol., 174:3818-3821.

Hutchinson, C. R.; Decker, H.; Motamedi, H.; Shen, B.; Summers, R. G.; Wendt-Pienkowski, R.; Wessel, W. L. (1992) Molecular Genetics and Biochemistry of Type II Polyketide Synthesis from the Perspective of Tetracenomycin C Biosynthesis in Streptomyces glaucescens. Actinomycetol., 6:49-68.

Hutchinson, C. R.; Decker, H.; Guilfoile, P. G.; Shen, B.; Summers, R. G.; Wendt-Pienkowski, E.; Wessel. W. L. (1992) Polyketide Synthase: Enzyme Complexes and Multifunctional Proteins Directing the Biosynthesis of Bacterial Metabolites from Fatty Acids, in Secondary Metabolite Biosynthesis and Metabolism, R. J. Petroski and S. P. McCormick, eds., Plenum Press, New York, pp. 3-10.

Hutchinson, C. R.; Decker, H.; Guilfoile, P. G.; Wendt-Pienkowski, E.; Shen, B.; Summers, R. G. (1992) Molecular Biology of Antibiotic Production in Streptomyces glaucescens, in Harnessing Biotechnol. 21st Century, Proc. Int. Biotechnol. Symp. Expo. 9th, M. R. Ladisch, and A. Bose, eds., ACS, Washington, D.C., pp. 127-130.

Hutchinson, C. R.; Decker, H.; Motamedi, H.; Shen, B.; Summers, R. G.; Wendt-Pienkowski, E.; Wessel, W. L. (1993) Molecular Genetics and Biochemistry of a Bacterial Type II Polyketide Synthase, in Industrial Microorganisms:  Basic and Applied Molecular Genetics, R. H. Baltz, G. D. Hegeman, P. L. Skatrud, eds., ASM, Washington, D.C., pp. 203-216.

Hutchinson, C. R.; Summers, R. G.; Shen, B.; Decker, H.; Wendt-Pienkowski, E.; Ali, A.; Grimm, A. C.; Suh, J. W.; Kwon, H. J.; and Wessel, W. L. (1993) Where Has the Manipulation of Bacterial Type II Polyketide Synthase Gene Led Us?  In Third International Conference on the Biotechnology of Microbial Products:  Novel Pharmacological and Agrobiological Activities, V. P. Gullo, J. C. Hunter-Cevera, R. Cooper, and R. K. Johnson, eds., SIM, Fredericksburg, Virginia, pp. 187-204.

Shen, B.; Nakayama, H.; Hutchinson, C. R. (1993) Isolation and Structural Elucidation of Tetracenomycin F2 and Tetracenomycin Fl: Early Intermediates in the Biosynthesis of Tetracenomycin C in Streptomyces glaucescens.  J. Nat. Prod., 56:1288-1293.

Shen, B.; Hutchinson, C. R. (1993) Tetracenomycin F1 Monooxygenase:  Oxidation of a Naphthacenone to a Naphthacenequinone in the Biosynthesis of Tetracenomycin C in Streptomyces glaucescens.  Biochemistry, 32:6656-6663.

Shen, B.; Hutchinson, C. R. (1993) Enzymatic Synthesis of a Bacterial Polyketide from Acetyl and Malonyl CoA by a Type II Polyketide Synthase.  Science, 262:1535-1540.

Shen, B.; Hutchinson, C. R. (1993) Tetracenomycin F2 Cyclase:  Intramolecular Aldol Condensation in the Biosynthesis of Tetracenomycin C in Streptomyces glaucescens.  Biochemistry, 32:11149-11154.

Shen, B.; Hutchinson, C. R. (1994) Triple-Hydroxylation of Tetracenomycin A2 to Tetracenomycin C in "Streptomyces glaucescens: Overexpression of the tcmG Gene in Streptomyces lividans  and Characterization of the Tcm A2 Oxygenase.  J. Biol. Chem., 48:30726-30733.

Shen, B.; Summers, R. G.; Wendt-Pienkowski, E.; Hutchinson, C. R. (1995) The Streptomyces glaucescens  tcmKL Polyketide Synthase and tcmN Polyketide Cyclase Genes Govern the Size and Shape of Aromatic Polyketides.  J. Am . Chem. Soc., 117:6811-6821.

Summers, R. G.; Ali, A.; Shen, B.; Wessel, W. L.; Hutchinson, C. R. (1995) Malonyl-Coenzyme A:Acyl Carrier Protein Acyltransferase of Streptomyces glaucescens: A Possible Link between Fatty Acid and Polyketide Biosynthesis.  Biochemistry, 34:9389-9402.

Shen, B.; Hutchinson, C. R. (1996) Deciphering the Mechanism for the Assembly of Aromatic Polyketides by a Bacterial Polyketide Synthase.  Proc. Natl. Acad. Sci. U.S.A., 93:6600-6604.

1986-1990 Publications

Liu, C.; Chen, Q.; Yu, Z.; Shen, B. (1986) Mordenite-Catalyzed Three-Phase Hydration of α-Pinene: Product Distribution, Kinetics and Reaction Mechanism. Huaxue Xuebo, 44:545-50.

Gould, S. J.;  Shen, B.; Whittle, Y. G.  (1989) The Biosynthesis of LL-C10037α:  The Steps beyond 3-Hydroxyanthranillic Acid. J. Am. Chem. Soc. 111:7932-38.

Shen, B.; Whittle, Y. G.;  Keszler, D. A.; Gould, S. J. (1990) Structure and Absolute Stereochemistry of the Epoxyquinol LL-C10037α and Related Metabolites from Streptomyces LL-C10037. J. Org. Chem., 55:4422-4426.

Gould, S. J.; Shen, B. (1991) Epoxyquinones from 2,5-Dihydroxyacetanilide: Opposite Facial Specificity in the Epoxydation by Enzymes from Streptomyces LL-C10037 and Streptomyces MPP 3051. J. Am. Chem. Soc., 113:684-686.

Shen, B.; Gould, S. J. (1991) Opposite Facial Specificity for Two Hydroquinone Epoxidases: (3-si, 4-re)-2,5-Dihydroxyacetanilide Epoxidase from Streptomyces LL-C10037 and (3-re, 4-si)-2,5-Dihydroxyacetanilide Epoxidase from Streptomyces MPP 3051". Biochemistry, 30:8937-8944.