Protocols for Project 1: Gene Expression Profiling

All protocols are in PDF format

DNA Isolation:

• DNA CPT v1 - Isolation of DNA from whole blood using BD Vacutainer Cell Preparation Tube with Sodium Citrate
• DNA Purple Top v1 - Isolation of DNA from whole blood using Vacutainer Purple Top Tube containing EDTA

RNA Isolation:

• RNA PAX v1 - Isolation of RNA from whole blood using Qiagen PAXgene Blood RNA Tube
• RNA CPT v1 - Isolation of RNA from whole blood using BD Vacutainer Cell Preparatiion Tube with Sodium Citrate

Protein Isolation:

• Protein CPT v1 - Isolation of protein from whole blood using BD Vacutainer Cell Preparation Tube with Sodium Citrate

 

All TGCG protocols can be found at the Transplant Genomics Homepage.