Protocols

RNA Isolation:

RNA Extraction Protocol, Thomas Whisenant

RNA Clean-up:

RNA Clean-Up Protocol, Affymetrix

RNA Amplification & Labeling:

Several RNA amplification and labeling protocols are now available and used in the DNA Array Core Facility. Depending on your experimental design and the amount of starting material you have, an appropriate protocol will be chosen. We highly recommend contacting the Core Facility to discuss the appropriate labeling protocol before beginning your experiment.

MessageAmp II-Biotin Enhanced Single Round aRNA Amplification Kit, Ambion

GeneChip Expression 3' Amplification One-Cycle Target Labeling, Affymetrix

WT-Ovation RNA Amplification System, NuGEN

Reduced Volume/Small Sample RNA Amplification Protocol, Baugh/Harvard