Scripps Research Logo

Molecular Medicine

Faculty

Timothy Spicer

Assistant Professor of Molecular Medicine
Associate Scientific Director
Department of Molecular Medicine
Florida Campus
Laboratory Website
spicert@scripps.edu
(561) 228-2150

Research Focus

The focus of our research involves enabling technologies for High Throughput Screening (HTS) of any target of unmet therapeutic need. Our facility supports scientist and faculty locally as well as those in the US and throughout the world. We do this by implementing their biological applications into high density plates for HTS. The breadth of biology we deal with is virtually unlimited due to the diversity of skill sets of the scientist and engineers within our group as well as the expanded capability of the readers associated to our system. We operate a fully automated 1536 well compatible platform and perform HTS on large compound libraries such as the Scripps Drug Discovery Library (645K) or small focused collections (FDA approved drugs). We have expertise in microfluidics, low nanoliter volume liquid handling, ultra-sensitive plate reader technologies and informatics set-up to handle large volume data sets. Key components are high-content readers, imaging detectors such as the ViewLux and FLIPR Tetra, as well as pintool and acoustic transfer devices. Through collaborative efforts we have successfully translated multiple small molecules into the clinics. We are open to any pharmacology desired (agonist, PAMs, NAMs, inverse agonist, etc.) vs. kinases, GPCR, NHRs, proteases, phenotypic assays, etc. which can be applied to any therapeutic area; i.e. CNS, infectious diseases, cardiovascular, oncology, metabolic diseases. In addition, we are currently funded (R33CA206949) to develop 3D spheroid based uHTS assays for the purpose of testing molecules in a more phenotypically relevant format to cancer biology.

Education

Ph.D., (Medicine), University of Queensland Australia, 2018
M.S., (Microbiology), State University of New York Health Science Center, 1996
B.S., (Biology), State University of New York at Albany, 1990

Professional Experience

1990-1996 Research Technician II, Department of Medicine, State University of New York Health Science Center at Syracuse
1996-2000 Associate Research Scientist II, Department of Virology HIV-1 Novel Targets, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut
2000-2003 Research Scientist I, Discovery Technologies, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut
2003-2005 Research Scientist II, Lead Discovery and Profiling, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut
2005-2013 Senior Staff Scientist, Lead Identification, Scripps Florida, Jupiter, FL
2013-Present Director of Lead Identification Discovery Biology and HTS, Scripps Florida, Jupiter, FL

Awards & Professional Activities

Bristol-Myers Squibb President's Award
Delivered the World's First Ultra High Throughput Screening System at BMS
Bristol-Myers Squibb Applied Biotechnology Collaboration Award
Bristol-Myers Squibb Triumph Award
Bristol-Myers Squibb Applied Biotechnology Collaboration Award
Inducted into the SLAS Fellows (2017)


2015 SLAS, Associate Track Chair, Automation and HTS Technologies
2016 SLAS Track Chair, Automation and HTS Technologies
2017 NIH SBIR Study Section Reviewer
2018 SLAS Conference Co-Chair
Journal of Biomolecular Screening Editorial Board

Selected References

Collia D, Bannister TD, Tan H, Jin S, Langaee T, Shumate J, Scampavia L,
Spicer TP. A Rapid Phenotypic Whole-Cell Screening Approach for the
Identification of Small-Molecule Inhibitors That Counter β-Lactamase Resistance
in Pseudomonas aeruginosa. SLAS Discov. 2017 Aug 1:2472555217728489. doi:
10.1177/2472555217728489. [Epub ahead of print] PubMed PMID: 28850797.

Janovick JA, Spicer TP, Bannister TD, Scampavia L, Conn PM. Pharmacoperone
rescue of vasopressin 2 receptor mutants reveals unexpected constitutive activity
and coupling bias. PLoS One. 2017 Aug 2;12(8):e0181830. doi:
10.1371/journal.pone.0181830. eCollection 2017. PubMed PMID: 28767678.

Wang J, Fang P, Chase P, Tshori S, Razin E, Spicer TP, Scampavia L, Hodder P,
Guo M. Development of an HTS-Compatible Assay for Discovery of Melanoma-Related
Microphthalmia Transcription Factor Disruptors Using AlphaScreen Technology. SLAS
Discov. 2017 Jan;22(1):58-66. doi: 10.1177/1087057116675274. Epub 2016 Nov 11.
PubMed PMID: 27827304.

Madoux F, Tanner A, Vessels M, Willetts L, Hou S, Scampavia L, Spicer TP. A
1536-Well 3D Viability Assay to Assess the Cytotoxic Effect of Drugs on
Spheroids. SLAS discovery. 2017; :2472555216686308. PubMed [journal] PMID:
28346088

Hou S, Madoux F, Scampavia L, Janovick JA, Conn PM, Spicer TP. Drug Library
Screening for the Identification of Ionophores That Correct the Mistrafficking
Disorder Associated with Oxalosis Kidney Disease. SLAS discovery. 2017;
:2472555217689992. PubMed [journal] PMID: 28346094

Janovick JA, Spicer TP, Smith E, Bannister TD, Kenakin T, Scampavia L, Conn PM.
Receptor antagonism/agonism can be uncoupled from pharmacoperone activity.
Molecular and cellular endocrinology. 2016; 434:176-85. NIHMSID: NIHMS801618
PubMed [journal] PMID: 27389877, PMCID: PMC4983495

Thenin-Houssier S, de Vera IM, Pedro-Rosa L, Brady A, Richard A, Konnick B,
Opp S, Buffone C, Fuhrmann J, Kota S, Billack B, Pietka-Ottlik M, Tellinghuisen
T, Choe H, Spicer T, Scampavia L, Diaz-Griffero F, Kojetin DJ, Valente ST.
Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication. Antimicrob
Agents Chemother. 2016 Mar 25;60(4):2195-208. doi: 10.1128/AAC.02574-15. Print
2016 Apr. PubMed PMID: 26810656; PubMed Central PMCID: PMC4808204.

Smith E, Janovick JA, Bannister TD, Shumate J, Scampavia L, Conn PM, Spicer TP.
Identification of Potential Pharmacoperones Capable of Rescuing the Functionality
of Misfolded Vasopressin 2 Receptor Involved in Nephrogenic Diabetes Insipidus.
Journal of biomolecular screening. 2016; 21(8):824-31. PubMed [journal] PMID:
27280550

Ewing H, Fernández-Vega V, Spicer TP, Chase P, Brown S, Scampavia L, Roush WR,
Riley S, Rosen H, Hodder P, Lambeau G, Gelb MH. Fluorometric High-Throughput
Screening Assay for Secreted Phospholipases A2 Using Phospholipid Vesicles.
Journal of biomolecular screening. 2016; 21(7):713-21. PubMed [journal] PMID:
27146384

Jambrina E, Cerne R, Smith E, Scampavia L, Cuadrado M, Findlay J, Krambis MJ,
Wakulchik M, Chase P, Brunavs M, Burris KD, Gallagher P, Spicer TP, Ursu D. An
Integrated Approach for Screening and Identification of Positive Allosteric
Modulators of N-Methyl-D-Aspartate Receptors. Journal of biomolecular screening.
2016; 21(5):468-79. PubMed [journal] PMID: 26838761

Palde PB, Bhaskar A, Pedró Rosa LE, Madoux F, Chase P, Gupta V, Spicer T,
Scampavia L, Singh A, Carroll KS. First-in-Class Inhibitors of Sulfur Metabolism
with Bactericidal Activity against Non-Replicating M. tuberculosis. ACS chemical
biology. 2016; 11(1):172-84. NIHMSID: NIHMS751325 PubMed [journal] PMID:
26524379, PMCID: PMC4729198

Chase P, Enogieru I, Madoux F, Bishop E, Beer J, Scampavia L, Spicer T. An
Automated Miniaturized Method to Perform and Analyze Antimicrobial Drug Synergy
Assays. Assay and drug development technologies. 2016; 14(1):58-66. PubMed
[journal] PMID: 26669516, PMCID: PMC4761817

Pedró-Rosa L, Buckner FS, Ranade RM, Eberhart C, Madoux F, Gillespie JR, Koh CY,
Brown S, Lohse J, Verlinde CL, Fan E, Bannister T, Scampavia L, Hol WG, Spicer T,
Hodder P. Identification of potent inhibitors of the Trypanosoma brucei
methionyl-tRNA synthetase via high-throughput orthogonal screening. Journal of
biomolecular screening. 2015; 20(1):122-30. NIHMSID: NIHMS670749 PubMed [journal]
PMID: 25163684, PMCID: PMC4378865

Smith E, Chase P, Niswender CM, Utley TJ, Sheffler DJ, Noetzel MJ, Lamsal A, Wood
MR, Conn PJ, Lindsley CW, Madoux F, Acosta M, Scampavia L, Spicer T, Hodder P.
Application of Parallel Multiparametric Cell-Based FLIPR Detection Assays for the
Identification of Modulators of the Muscarinic Acetylcholine Receptor 4 (M4).
Journal of biomolecular screening. 2015; 20(7):858-68. NIHMSID: NIHMS735334
PubMed [journal] PMID: 25877150, PMCID: PMC4659430

Conn PM, Spicer TP, Scampavia L, Janovick JA. Assay strategies for identification
of therapeutic leads that target protein trafficking. Trends in pharmacological
sciences. 2015; 36(8):498-505. NIHMSID: NIHMS693772 PubMed [journal] PMID:
26067100, PMCID: PMC4532603

Chang JW, Zuhl AM, Speers AE, Niessen S, Brown SJ, Mulvihill MM, Fan YC, Spicer
TP, Southern M, Scampavia L, Fernandez-Vega V, Dix MM, Cameron MD, Hodder PS,
Rosen H, Nomura DK, Kwon O, Hsu KL, Cravatt BF. Selective inhibitor of
platelet-activating factor acetylhydrolases 1b2 and 1b3 that impairs cancer cell
survival. ACS chemical biology. 2015; 10(4):925-32. NIHMSID: NIHMS665008 PubMed
[journal] PMID: 25602368, PMCID: PMC4402257

Links

Scripps News & Views: Scripps Florida Scientists Awarded $2 Million to Develop ‘Industrial Level’ Screening for Treatments of Autism-Related Intellectual Disability

Science: Adding depth to cell culture

Making Plans: Scripps Florida Screening Center Expands Its Collaborations

Using bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates

Molecular Medicine Seminar Series

CA Faculty

Asahara, Hiroshi
Balch, William E.
Boddy, Michael
Buxbaum, Joel
Catz, Sergio
Conti, Bruno
Cravatt, Benjamin
D'Lima, Darryl
DerMardirossian, Celine
Deryugina, Elena
Elias, Darlene
Encalada, Sandra
Felding, Brunhilde
Fowler, Velia
Friedlander, Martin
Gerace, Larry
Gottesfeld, Joel
Griffin, John
Hanneken, Anne
Heeb, Mary Jo
Henderson, Scott
Hoch, James
Joazeiro, Claudio
Johnson, Eric
Kanaji, Taisuke
Kay, Steve
Kelly, Jeffery
Koziol, James
Lamia, Katja
Lazzerini Denchi, Eros
Lipton, Stuart
Loring, Jeanne F.
Loskutoff, David
Lotz, Martin
Makarenkova, Helen P.
Mc Millan, Robert
Miles, Lindsey
Milner, Richard
Modena, Brian
Mosnier, Laurent
Paulson, James C.
Perego, Marta
Petrascheck, Michael
Pollard, Kenneth Michael
Quigley, James P.
Reed, Steven
Roberts, Edward
Rosen, Hugh
Ruggeri, Zaverio
Russell, Paul
Saez, Enrique
Smider, Vaughn
Srinivasan, Supriya
Steinhubl, Steven
Sutcliffe, J.
Tan, Eng
Topol, Eric
Vanderklish, Peter
Vogt, Peter K.
Waalen, Jill
Wiseman, Luke
Wittenberg, Curt
Wolan, Dennis W.
Wu, Peng
Wu, Xiaohua
Yang, Xiang-Lei
Yates III, John


FL Faculty

Bohn, Laura M.
Cameron, Michael
Duckett, Derek
Gill, Matt
Griffin, Patrick
Hansen, Scott
Kamenecka, Theodore
Kissil, Joseph
Luo, Junli
McDonald, Patricia
Miller, Courtney
Niedernhofer, Laura
Phinney, Donald G.
Robbins, Paul
Scampavia, Louis
Schimmel, Paul
Smith, Roy
Spicer, Timothy


Affiliated Faculty

Cline, Hollis
Kojetin, Douglas
Lairson, Luke
Paegel, Brian
Rumbaugh, Gavin
Shen, Ben
Solt, Laura A
Su, Andrew
Wu, Chunlei


Adjunct Faculty