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Molecular Medicine

Faculty

Louis Scampavia, Ph.D.

Associate Professor of Molecular Medicine
Translational Research Institute
Florida Campus
Laboratory Website
scampl@scripps.edu
(561) 228-2101

Research Focus

Progress in drug discovery is often coupled to parallel advancements in instrument technology. In our department, High Throughput Screening (HTS) robotics is employed to accelerate the drug discovery process thorough full automation of large scale screening experiments. HTS technology can be used to execute and analyze hundreds of thousand of experiments against large compound libraries to identify a few select compounds of therapeutic value. These "therapeutic leads" provide important insight and basis for the medicinal development of novel drugs for clinical application.

 Research efforts are currently focused on:

  • Working with collaborators to develop biological assays for HTS compatibility.
  • Performing exploratory HTS campaigns for drug lead discovery.
  • Research and development of novel instruments and technologies for the advancement of HTS robotics.

Collaborative efforts are coordinated with a large community of scientists and include TSRI faculty, Florida researchers, and scientists funded through the National Institutes of Health sponsored Molecular Library Screening Center Network program (http://nihroadmap.nih.gov/).

Professional Experience

Awards & Professional Activities

Selected References

Collia D, Bannister TD, Tan H, Jin S, Langaee T, Shumate J, Scampavia L,
Spicer TP. A Rapid Phenotypic Whole-Cell Screening Approach for the
Identification of Small-Molecule Inhibitors That Counter β-Lactamase Resistance
in Pseudomonas aeruginosa. SLAS Discov. 2017 Aug 1:2472555217728489. doi:
10.1177/2472555217728489. [Epub ahead of print] PubMed PMID: 28850797.

Thenin-Houssier S, de Vera IM, Pedro-Rosa L, Brady A, Richard A, Konnick B,
Opp S, Buffone C, Fuhrmann J, Kota S, Billack B, Pietka-Ottlik M, Tellinghuisen
T, Choe H, Spicer T, Scampavia L, Diaz-Griffero F, Kojetin DJ, Valente ST.
Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication. Antimicrob
Agents Chemother. 2016 Mar 25;60(4):2195-208. doi: 10.1128/AAC.02574-15. Print
2016 Apr. PubMed PMID: 26810656; PubMed Central PMCID: PMC4808204.

Wang J, Fang P, Chase P, Tshori S, Razin E, Spicer TP, Scampavia L, Hodder P, Guo
M. Development of an HTS-Compatible Assay for Discovery of Melanoma-Related
Microphthalmia Transcription Factor Disruptors Using AlphaScreen Technology. SLAS
discovery. 2017; 22(1):58-66. PubMed [journal] PMID: 27827304

Madoux F, Tanner A, Vessels M, Willetts L, Hou S, Scampavia L, Spicer TP. A
1536-Well 3D Viability Assay to Assess the Cytotoxic Effect of Drugs on
Spheroids. SLAS discovery. 2017; :2472555216686308. PubMed [journal] PMID:
28346088

Wang J, Fang P, Chase P, Tshori S, Razin E, Spicer TP, Scampavia L, Hodder P, Guo
M. Development of an HTS-Compatible Assay for Discovery of Melanoma-Related
Microphthalmia Transcription Factor Disruptors Using AlphaScreen Technology. SLAS
discovery. 2017; 22(1):58-66. PubMed [journal] PMID: 27827304

Hou S, Madoux F, Scampavia L, Janovick JA, Conn PM, Spicer TP. Drug Library
Screening for the Identification of Ionophores That Correct the Mistrafficking
Disorder Associated with Oxalosis Kidney Disease. SLAS discovery. 2017;
:2472555217689992. PubMed [journal] PMID: 28346094

Madoux F, Dreymuller D, Pettiloud JP, Santos R, Becker-Pauly C, Ludwig A, Fields
GB, Bannister T, Spicer TP, Cudic M, Scampavia LD, Minond D. Discovery of an
enzyme and substrate selective inhibitor of ADAM10 using an exosite-binding
glycosylated substrate. Scientific reports. 2016; 6(1):11. PubMed [journal] PMID:
28442704

Madoux F, Dreymuller D, Pettiloud JP, Santos R, Becker-Pauly C, Ludwig A, Fields
GB, Bannister T, Spicer TP, Cudic M, Scampavia LD, Minond D. Discovery of an
enzyme and substrate selective inhibitor of ADAM10 using an exosite-binding
glycosylated substrate. Scientific reports. 2016; 6(1):11. PubMed [journal] PMID:
27920432

Janovick JA, Spicer TP, Smith E, Bannister TD, Kenakin T, Scampavia L, Conn PM.
Receptor antagonism/agonism can be uncoupled from pharmacoperone activity.
Molecular and cellular endocrinology. 2016; 434:176-85. NIHMSID: NIHMS801618
PubMed [journal] PMID: 27389877, PMCID: PMC4983495

Jambrina E, Cerne R, Smith E, Scampavia L, Cuadrado M, Findlay J, Krambis MJ,
Wakulchik M, Chase P, Brunavs M, Burris KD, Gallagher P, Spicer TP, Ursu D. An
Integrated Approach for Screening and Identification of Positive Allosteric
Modulators of N-Methyl-D-Aspartate Receptors. Journal of biomolecular screening.
2016; 21(5):468-79. PubMed [journal] PMID: 26838761

Plate L, Cooley CB, Chen JJ, Paxman RJ, Gallagher CM, Madoux F, Genereux JC,
Dobbs W, Garza D, Spicer TP, Scampavia L, Brown SJ, Rosen H, Powers ET, Walter P,
Hodder P, Wiseman RL, Kelly JW. Small molecule proteostasis regulators that
reprogram the ER to reduce extracellular protein aggregation. eLife. 2016; 5.
PubMed [journal] PMID: 27435961, PMCID: PMC4954754

Ewing H, Fernández-Vega V, Spicer TP, Chase P, Brown S, Scampavia L, Roush WR,
Riley S, Rosen H, Hodder P, Lambeau G, Gelb MH. Fluorometric High-Throughput
Screening Assay for Secreted Phospholipases A2 Using Phospholipid Vesicles.
Journal of biomolecular screening. 2016; 21(7):713-21. PubMed [journal] PMID:
27146384

Smith E, Janovick JA, Bannister TD, Shumate J, Scampavia L, Conn PM, Spicer TP.
Identification of Potential Pharmacoperones Capable of Rescuing the Functionality
of Misfolded Vasopressin 2 Receptor Involved in Nephrogenic Diabetes Insipidus.
Journal of biomolecular screening. 2016; 21(8):824-31. PubMed [journal] PMID:
27280550

Janovick JA, Spicer TP, Bannister TD, Scampavia L, Conn PM. Pharmacoperone
rescue of vasopressin 2 receptor mutants reveals unexpected constitutive activity
and coupling bias. PLoS One. 2017 Aug 2;12(8):e0181830. doi:
10.1371/journal.pone.0181830. eCollection 2017. PubMed PMID: 28767678.

Links

Scripps Florida Scientists Discover New Compounds with Potential to Treat Persistent Tuberculosis

Making Plans: Scripps Florida Screening Center Expands Its Collaborations

Using bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates

Molecular Medicine Seminar Series

CA Faculty

Asahara, Hiroshi
Balch, William E.
Boddy, Michael
Buxbaum, Joel
Catz, Sergio
Conti, Bruno
Cravatt, Benjamin
D'Lima, Darryl
DerMardirossian, Celine
Deryugina, Elena
Elias, Darlene
Encalada, Sandra
Felding, Brunhilde
Fowler, Velia
Friedlander, Martin
Gerace, Larry
Gottesfeld, Joel
Griffin, John
Hanneken, Anne
Heeb, Mary Jo
Henderson, Scott
Hoch, James
Joazeiro, Claudio
Johnson, Eric
Kanaji, Taisuke
Kay, Steve
Kelly, Jeffery
Koziol, James
Lamia, Katja
Lazzerini Denchi, Eros
Lipton, Stuart
Loring, Jeanne F.
Loskutoff, David
Lotz, Martin
Makarenkova, Helen P.
Mc Millan, Robert
Miles, Lindsey
Milner, Richard
Modena, Brian
Mosnier, Laurent
Paulson, James C.
Perego, Marta
Petrascheck, Michael
Pollard, Kenneth Michael
Quigley, James P.
Reed, Steven
Roberts, Edward
Rosen, Hugh
Ruggeri, Zaverio
Russell, Paul
Saez, Enrique
Smider, Vaughn
Srinivasan, Supriya
Steinhubl, Steven
Sutcliffe, J.
Tan, Eng
Topol, Eric
Vanderklish, Peter
Vogt, Peter K.
Waalen, Jill
Wiseman, Luke
Wittenberg, Curt
Wolan, Dennis W.
Wu, Peng
Wu, Xiaohua
Yang, Xiang-Lei
Yates III, John


FL Faculty

Bohn, Laura M.
Cameron, Michael
Duckett, Derek
Gill, Matt
Griffin, Patrick
Hansen, Scott
Kamenecka, Theodore
Kissil, Joseph
Luo, Junli
McDonald, Patricia
Miller, Courtney
Niedernhofer, Laura
Phinney, Donald G.
Robbins, Paul
Scampavia, Louis
Schimmel, Paul
Smith, Roy
Spicer, Timothy


Affiliated Faculty

Cline, Hollis
Kojetin, Douglas
Lairson, Luke
Paegel, Brian
Rumbaugh, Gavin
Shen, Ben
Solt, Laura A
Su, Andrew
Wu, Chunlei


Adjunct Faculty