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Molecular Medicine

Faculty

Joel Buxbaum, M.D.

Professor Emeritus
Department of Molecular Medicine
California Campus
Laboratory Website
jbux@scripps.edu
(858) 784-8885

Scripps Research Joint Appointments

The Keck Autoimmune Disease Center

Research Focus

Molecular Basis of Acquired and Hereditary Human Disease

In the human amyloidoses, soluble proteins, synthesized and secreted from the cell, become insoluble in tissues with resultant organ compromise. The serum protein transthyretin is the precursor to the deposited protein in late-onset hereditary and acquired forms of neurologic, heart and kidney amyloidosis. We have established a disease model by creating a strain of mice carrying the human gene. In humans, amyloid deposition requires many years for initiation and progression. In our transgenic mice, by two years of age, a majority develop age-dependent deposition of the human protein in the heart and/or kidneys, a delay consistent with the late onset seen in humans. Prior to the appearance of Congophilic fibrils, the animals display less structured deposits that appear to be precursors to the amyloid fibrils. Analysis of this apparent in vivo folding intermediate should provide insight into the pathologic process responsible for the human disease. Our studies are designed to determine why deposition is age-dependent and what factors modulate the changes in protein conformation from the non-fibrillar to the fibrillar state.

Education

M.D., Medicine, Tufts University, 1962
B.S., Union College, 1958

Professional Experience

Awards & Professional Activities

1980-1981, American Cancer Society Scholar in Cancer Research


1997-2000, Frank G. Spencer Fellow New York Affiliate American Heart Association


2000, Edward Bierman Lecturer Mid-Winter Conference on Controversies in Medicine


2011, Ellison Medical Foundation Senior Scholar Award


2013, Dart-NYU Biotechnology Award


 

Selected References

Wang,X.; Cattaneo,F.; Ryno,L.; Hulleman,J.; Reixach,N.; Buxbaum,J.N. The Systemic Amyloid Precursor Transthyretin (TTR) Behaves as a Neuronal Stress Protein Regulated by HSF1 in SH-SY5Y Human Neuroblastoma Cells and APP23 Alzheimer's Disease Model Mice. J Neurosci. 2014 May:34 (21) 7253-65. PMID:24849358.

Cascella,R.; Conti,S.; Mannini,B.; Li,X.; Buxbaum,J.N.; Tiribilli,B.; Chiti,F.; Cecchi,C. Transthyretin suppresses the toxicity of oligomers formed by misfolded proteins in vitro Biochim.Biophys.Acta. 2013 Sept. 1832:(12):2302-14. PMID:24075940

Li, X, Zhang X, Yadav JK, Du D, Wright PE, Kelly JW, Buxbaum, JN. Mechanisms of transthyretin inhibition of β-amyloid aggregation: the roles of the thyroxine binding site and tetramer stability J Neurosci. 2013 Dec 11;33(50):19423-33. PMID:24336709.

Zhao L, Buxbaum JN, Reixach Natàlia, Age-related oxidative modifications of transthyretin increase its amyloidogenicity. Biochemistry 2013 Mar 19;52(11):1913-26. PMID:23414091.

Buxbaum, Joel N., Tagoe, C., Gallo, G., Walker, J.R., Kurian, S., Salomon, D.R. Why are some amyloidosis systemic? Does hepatic "chaperoning at a distance: prevent cardiac deposition in a transgenic model of human senile systemic (transthyretin) amyloidosis? FASEB J. 2012 Jun;26 (6):2283-93. PMID:22362898.

Li, X., Masliah E., Reixach N., Buxbaum, Joel N.The systemic amyloid precursor transthyretin inhibits Alzheimer neurotoxicity in vivo and in tissue culture. J Neurosci 31:12483-90, 2011. PMCID: PMC3172869

Jacobson D, Tagoe, C, Schwarzbard, A, Shah, A, Koziol, J, Buxbaum, JN. Relationship of Clinical, Echocardiographic and Electrocardiographic Features of Cardiac Amyloidosis to the Presence of the Transthyretin V122I Allele in Older African-American Males. Am J Cardiol 108:440-4, 2011. PMID:21600538.

Bourgault S, Choi S, Buxbaum JN, Kelly JW, Price JL, Reixach N. Mechanisms of transthyretin cardiotoxicity inhibition by resveratrol analogs. Biochem Biophys Res Commun 410:707-13, 2011. PMCID: PMC3145458.

Waalen J. and Buxbaum, JN. Is Older Colder or Colder Older? The Association of Age with Body Temperature in 18,630 Individuals. J Gerontol A Biol Sci Med Sci , 2011 Epub. PMID:2132495

Buxbaum J, Alexander A, Koziol J, Tagoe C, Fox E, Kitzman D. Significance of the amyloidogenic transthyretin Val 122 Ile allele in African Americans in the Arteriosclerosis Risk in Communities (ARIC) and Cardiovascular Health (CHS) Studies. Am Heart J. 2010 May; 159(5):864-70. PMID: 20435197.

Soares ML, Coelho T, Sousa A, Batalov S, Conceição I, Sales-Luís ML, Ritchie MD, Williams SM, Nievergelt CM, Schork NJ, Saraiva MJ, Buxbaum JN. Susceptibility and modifier genes in Portuguese transthyretin V30M amyloid polyneuropathy: complexity in a single gene disease. Hum. Mol. Gen. 14(4):543-553, 2005.

Reixach N, Deechongkit S, Foss T, Jiang X, Kelly JW, Buxbaum JN. Tissue damage in the amyloidoses: Transthyretin monomers and nonnative oligomers are the major cytotoxic species in tissue culture. Proc Natl Acad Sci (USA) 101(9):2817-2822, 2004.

Buxbaum JN. Diseases of protein conformation: what do in vitro experiments tell us about the in vivo diseases? Trends in Biochemical Sciences 28 (11):585-92, 2003.

Teng MH, Yin J, Vidal R, Ghiso J, Tagoe C, Gallo G and Buxbaum JN: Amyloid and non-fibrillar tissue deposits in animals transgenic for wild type human transthyretin: A possible model for senile systemic amyloidosis. Laboratory Investigation 81:385-396, 2001.

Molecular Medicine Seminar Series

CA Faculty

Asahara, Hiroshi
Balch, William E.
Boddy, Michael
Buxbaum, Joel
Catz, Sergio
Conti, Bruno
Cravatt, Benjamin
D'Lima, Darryl
DerMardirossian, Celine
Deryugina, Elena
Elias, Darlene
Encalada, Sandra
Felding, Brunhilde
Fowler, Velia
Friedlander, Martin
Gerace, Larry
Gottesfeld, Joel
Griffin, John
Hanneken, Anne
Heeb, Mary Jo
Henderson, Scott
Hoch, James
Joazeiro, Claudio
Johnson, Eric
Kanaji, Taisuke
Kay, Steve
Kelly, Jeffery
Koziol, James
Lamia, Katja
Lazzerini Denchi, Eros
Lipton, Stuart
Loring, Jeanne F.
Loskutoff, David
Lotz, Martin
Makarenkova, Helen P.
Mc Millan, Robert
Miles, Lindsey
Milner, Richard
Modena, Brian
Mosnier, Laurent
Paulson, James C.
Perego, Marta
Petrascheck, Michael
Pollard, Kenneth Michael
Quigley, James P.
Reed, Steven
Roberts, Edward
Rosen, Hugh
Ruggeri, Zaverio
Russell, Paul
Saez, Enrique
Smider, Vaughn
Srinivasan, Supriya
Steinhubl, Steven
Sutcliffe, J.
Tan, Eng
Topol, Eric
Vanderklish, Peter
Vogt, Peter K.
Waalen, Jill
Wiseman, Luke
Wittenberg, Curt
Wolan, Dennis W.
Wu, Peng
Wu, Xiaohua
Yang, Xiang-Lei
Yates III, John


FL Faculty

Bohn, Laura M.
Cameron, Michael
Duckett, Derek
Gill, Matt
Griffin, Patrick
Hansen, Scott
Kamenecka, Theodore
Kissil, Joseph
Luo, Junli
McDonald, Patricia
Miller, Courtney
Niedernhofer, Laura
Phinney, Donald G.
Robbins, Paul
Scampavia, Louis
Schimmel, Paul
Smith, Roy
Spicer, Timothy


Affiliated Faculty

Cline, Hollis
Kojetin, Douglas
Lairson, Luke
Paegel, Brian
Rumbaugh, Gavin
Shen, Ben
Solt, Laura A
Su, Andrew
Wu, Chunlei


Adjunct Faculty