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Molecular Medicine

Faculty

Michael Boddy, Ph.D.

Associate Professor
Department of Molecular Medicine
California Campus
Laboratory Website
nboddy@scripps.edu
(858) 784-7042

Scripps Research Joint Appointments

Faculty, Graduate Program

Research Focus

DNA Repair and the Maintenance of Genomic Stability

Our laboratory uses the genetically tractable fission yeast as a model system to study proteins involved in the maintenance of genomic stability. Loss of genomic integrity is causally associated with cancer and other diseases. The pathways that we study are highly conserved across species and thus provide a valuable framework for analysis of the analogous human pathways.

Currently we are studying functional interactions between proteins of the DNA repair and replication checkpoint systems. The replication checkpoint senses replication perturbations and coordinates cellular responses such as cell-cycle arrest, replication fork-stabilization and DNA repair. The enforcer of the replication checkpoint is a kinase called Cds1 (Human Chk2). The way in which Cds1 arrests cell-cycle progression when replication is blocked has been worked out in some detail. However, little is known about the targets of Cds1 that are important for replication fork stability and DNA repair. We have identified novel DNA repair factors that are regulated via Cds1-dependent phosphorylation. We are currently determining the precise molecular function(s) of these DNA repair proteins so that we can understand how their regulation by Cds1 mitigates genome damage.

Education

Ph.D., King's College, University of London & Cancer Research, UK., 1996

Awards & Professional Activities

Scholar of The Leukemia & Lymphoma Society

Selected References

For a complete list of publications: http://www.scripps.edu/boddy/publications.html

Groocock LM., Nie M., Prudden J., Moiani D., Wang T., Cheltsov A., Rambo RP., Arvai AS., Hitomi C., Tainer JA., Luger K., Perry JJ., Denchi-EL., Boddy MN.  RNF4 Interacts with both Small Ubiquitin-Like Modifier and Nucleosomes to Promote the DNA Damage Response.  EMBO Rep.; (in press), 2014.

Zilio N., Wehrkamp-Richter S., Boddy M.N.  (2012) A new versatile system for rapid control of gene expression in the fission yeast Schizosaccharomyces pombe. Yeast. 29(10):425-434.  PMCID: 3478897.

Wehrkamp-Richter S., Hyppa R.W., Prudden J., Smith G.R., Boddy M.N.  (2012) Meiotic DNA joint molecule resolution depends on Nse5-Nse6 of the Smc5-Smc6 holocomplex. Nucleic acids research. 40(19):9633-9646.  PMCID: 3479181

Nie M., Aslanian A., Prudden J., Heideker J., Vashisht A.A., Wohlschlegel J.A., Yates J.R., 3rd, Boddy M.N. (2012) Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions. J Biol Chem. 287(35):29610-29619.  PMCID: 3436128.

Groocock LM, Prudden J, Perry JJ, Boddy MN. (2012) RecQ4 Orthologue Hrq1 is Critical for DNA Interstrand Crosslink Repair and Genome Stability in Fission Yeast. Mol. Cell. Biol.; 32(2):276-287.

Prudden J, Perry JJ, Nie M, Vashisht AA, Arvai AS, Hitomi C, Guenther G, Wohlschlegel JA, Tainer JA, Boddy MN. (2011) DNA repair and global sumoylation are regulated by distinct ubc9 noncovalent complexes. Mol. Cell. Biol.; 31(11):2299-2310.

Heideker J, Prudden J, Perry JJ, Tainer JA, Boddy MN. (2011) SUMO-Targeted Ubiquitin Ligase, Rad60, and Nse2 SUMO Ligase Suppress Spontaneous Top1-Mediated DNA Damage and Genome Instability. PLoS Genet; 7(3):e1001320.

Prudden J, Perry JJ, Arvai AS, Tainer JA, Boddy MN. (2009) Molecular mimicry of SUMO promotes DNA repair. Nature Struct Mol Biol; 16(5):509-516. PMC2711901.
– Faculty of 1000
: Must Read FFa 9 (Faculty 1000).
– Scripps News Release:
(News & Views).

Pebernard S, Schaffer L, Campbell D, Head SR, Boddy MN. (2008) Localization of Smc5/6 to centromeres and telomeres requires heterochromatin and SUMO, respectively. EMBO J; 27(22):3011-3023. PMC2585169.

Pebernard S, Perry JJ, Tainer JA, Boddy MN. (2008) Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability. Mol. Biol. Cell; 19(10):4099-4109. PMC2555936.

Prudden J, Pebernard S, Raffa G, Slavin DA, Perry JJ, Tainer JA, McGowan CH, Boddy MN. (2007) SUMO-targeted ubiquitin ligases in genome stability. EMBO J; 26(18):4089-4101.
– Faculty 1000: Must Read FFa 8 (Faculty 1000).
– Scripps News Release: (News & Views).

Pebernard S, Wohlschlegel J, McDonald WH, Yates 3rd JR, Boddy MN. The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex. Mol. Cell. Biol. 26:1617, 2006.
– Faculty 1000: (Faculty 1000).

Molecular Medicine Seminar Series

CA Faculty

Asahara, Hiroshi
Balch, William E.
Boddy, Michael
Buxbaum, Joel
Catz, Sergio
Conti, Bruno
Cravatt, Benjamin
D'Lima, Darryl
DerMardirossian, Celine
Deryugina, Elena
Elias, Darlene
Encalada, Sandra
Felding, Brunhilde
Fowler, Velia
Friedlander, Martin
Gerace, Larry
Gottesfeld, Joel
Griffin, John
Hanneken, Anne
Heeb, Mary Jo
Henderson, Scott
Hoch, James
Joazeiro, Claudio
Johnson, Eric
Kanaji, Taisuke
Kay, Steve
Kelly, Jeffery
Koziol, James
Lamia, Katja
Lazzerini Denchi, Eros
Lipton, Stuart
Loring, Jeanne F.
Loskutoff, David
Lotz, Martin
Makarenkova, Helen P.
Mc Millan, Robert
Miles, Lindsey
Milner, Richard
Modena, Brian
Mosnier, Laurent
Paulson, James C.
Perego, Marta
Petrascheck, Michael
Pollard, Kenneth Michael
Quigley, James P.
Reed, Steven
Roberts, Edward
Rosen, Hugh
Ruggeri, Zaverio
Russell, Paul
Saez, Enrique
Smider, Vaughn
Srinivasan, Supriya
Steinhubl, Steven
Sutcliffe, J.
Tan, Eng
Topol, Eric
Vanderklish, Peter
Vogt, Peter K.
Waalen, Jill
Wiseman, Luke
Wittenberg, Curt
Wolan, Dennis W.
Wu, Peng
Wu, Xiaohua
Yang, Xiang-Lei
Yates III, John


FL Faculty

Bohn, Laura M.
Cameron, Michael
Duckett, Derek
Gill, Matt
Griffin, Patrick
Hansen, Scott
Kamenecka, Theodore
Kissil, Joseph
Luo, Junli
McDonald, Patricia
Miller, Courtney
Niedernhofer, Laura
Phinney, Donald G.
Robbins, Paul
Scampavia, Louis
Schimmel, Paul
Smith, Roy
Spicer, Timothy


Affiliated Faculty

Cline, Hollis
Kojetin, Douglas
Lairson, Luke
Paegel, Brian
Rumbaugh, Gavin
Shen, Ben
Solt, Laura A
Su, Andrew
Wu, Chunlei


Adjunct Faculty