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Daniel R. Salomon, M.D.

Professor
Director, Laboratory for Functional Genomics
Medical Program Director, Scripps Center for Organ and Cell Transplantation
Department of Molecular and Experimental Medicine
California Campus
Laboratory Website
dsalomon@scripps.edu
(858) 784-9381

Scripps Research Joint Appointments

Faculty, Graduate Program

Research Focus

The focus of our laboratory effort is the integrated study of transplantation immunology using the latest tools of functional genomics and molecular immunology. Transplantation for us spans the challenges of kidney and liver transplantation for end stage organ failure, islet transplantation for diabetes, adult stem cells for therapy of genetic models of organ failure and therapeutic gene delivery with retroviral vectors. Our functional genomics work includes whole genome transcription profiling from DNA microarrays to deep mRNA sequencing, alternative splicing arrays, microRNA studies (miRNA-seq), high-throughput next generation DNA sequencing (DNA-seq) including targeted sequencing and whole exome sequencing and transcription factor profiling (ChIP-seq), the epigenetics of immunology at the level of DNA methylation (methyl DNA-seq) and shot gun tandem mass spectrometry proteomics.

We have applied these tools to studying the molecular mechanisms of T and B cell activation and regulation, to detailed studies of transcriptional regulation, to studies of the phosphoproteome of activated lymphocytes, to developing novel blood cell biomarkers for predicting rejection and managing immunosuppressive therapy in patients with kidney transplants, to profiling liver transplants to understand the potential of tissue regeneration and to human islets to understand the molecular basis of a functional cell transplant. In all our work, the objective is to work in a multi-dimensional genomic space created by an ongoing and iterative integration of the latest technologies with cell-based, preclinical animal models and human clinical studies. At each step we strive to design studies that often move from clinical patient samples to cell assays that effectively test and validate basic molecular mechanisms and identify their regulatory elements in the context of what is known and what we can discover to basic molecular studies of mechanisms and structure. When this effort is successful, we can construct cell response systems and map the interactions of multiple genes into functional networks, both driving and regulating the immunity, inflammation, tissue injury and regeneration that determine the final outcomes of any cell or organ transplant.

Education

M.D., Medicine, Loyola University Chicago, 1976

Professional Experience

Northwestern University: Major in Chemistry
Stritch-Loyola School of Medicine: Medicine
Cedars-Sinai Medical Center, Los Angeles: Residency and Chief Resident
Brigham and Women's Hospital, Harvard Medical School: Nephrology and Transplantation Immunology Fellowships
National Institutes of Health, Laboratory of Immunology: Post-doctoral Training, Molecular Immunology

Awards & Professional Activities

Past Positions (last 5 years)
Chair, Biological Response Modifiers Advisory Committee, Food and Drug Administration
Member, Secretary of Health's Advisory Committee on Xenotransplantation
Chair, American Society of Gene Therapy Regulatory Affairs Committee
Chair, National Institutes of Health Islet Cell Resources Consortium

Current Positions
Chair, National Institutes of Health Transplantation Genomics Steering Committee
Chair, American Society of Gene Therapy Tissue Engineering Committee
Medical Program Director, Scripps Center for Organ and Cell Transplantation

Current Editorial Boards
American Journal of Transplantation
, Associate Editor
Transplantation Journal,
Associate Editor

 

Selected References

Flechner SM, Kurian SM, Head SR, Sharp SM, Whisenant TC, Zhang J, Chismar JD, Horvath S, Mondala T, Gilmartin T, Cook DJ, Kay SA, Walker JR and Salomon DR. Acute Kidney Transplant Rejection and Tissue Injury Revealed in Gene Profiling Signatures of Biopsies and Peripheral Blood Lymphocytes. American Journal of Transplantation, vol. 4(9):1475-89, 2004.

Martina Y, Marcucci K, Szabo A, Drysdale T, Wilson C, Patience C and Salomon DR. Mice transgenic for a human Porcine Endogenous Retroviral receptor are susceptible to productive viral infection. Journal of Virology, 80(7):3135-46, 2006.

Cherqui S, Thorpe C, Kurian SM and Salomon DR. Lentiviral gene delivery of vMIP-II to transplanted endothelial cells and endothelial progenitors is proangiogenic in vivo. (Molecular Therapy 15(7):1264-72, 2007.

Le-Niculescu H, Kurian SM, Yehyawi N, Dike C, Patel SD, Edenberg HJ, Tsuang MT, Salomon DR, Nurnberger Jr JI and Niculescu AB. Identifying blood biomarkers for mood disorders using convergent functional genomics. (Molecular Psychiatry, Advanced Online, 2008).

Grigoryev YA, Kurian SM, Avnur Z, Borie D, Deng J, Campbell D, Sung J, Nikolcheva T, Quinn A, Schulman H, Peng SL, Schaffer R, Fisher J, Mondala T, Head S, Flechner SM, Kantor AB, Marsh CL, and Salomon DR. Deconvoluting Post-Transplant Immunity: Subset-specific Mapping of Activation and Regulatory Programs in Lymphocytes and Monocytes. PLoS ONE, 5(10): e13358, 2010.

Nakorchevskiy A, Hewel JA, Kurian SM, Mondala TS, Campbell D, Head SR, Marsh CL, J Yates JRIII and Salomon DR. Molecular Mechanisms of Chronic Kidney Transplant Rejection and Tissue Injury Revealed by Large-Scale Proteogenomic Analysis of Tissue Biopsies. Journal of the American Society of Nephrology, 21(2):362-73, 2010. Epub 2010 Jan 21