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Scripps Research Joint Appointments

Center for Integrative Molecular Biosciences

Research Focus

The focus of our group is to develop a completely automated system for molecular microscopy using cryoEM. It is our goal to automate all processes from EM grid insertion to calculating the final 3D map. Over the past several years, we have developed a system, called Leginon, to automatically acquire cryo-electron micrographs. The system is designed to emulate all of the decisions and actions of a highly trained microscopist in collecting data from a vitreous ice specimen. Technologies within the group that form the basis for this system include instrument control and development, robotics, and automated image processing, analysis and reconstruction. The group includes engineers and computer scientists.

Awards & Professional Activities

Member, Microscopy Society of America

Selected References

Potter, Clinton S., James Pulokas, Paul Smith, Christian Suloway and Bridget Carragher. (2004) "Robotic Grid Loading System for a Transmission Electron Microscope", JSB, 146, 431-440.

Zhu, Yuanxin, Bridget Carragher, Robert M. Glaeser, Denis Fellmann, Chandrajit Bajaj, Marshall Bern, Fabrice Mouche, Felix de Haas, Richard J. Hall, Steven C. Ludtke, Pawel A. Penczek, Alan M. Roseman, Fred J. Sigworth, Niels Volkmann, and Clinton S. Potter. (2003) "Automatic Particle Selection: Results of A "Bakeoff"", JSB, 145, 3-14.

Carragher, B., D. Fellmann, F. Guerra, R.A. Milligan, F. Mouche, J. Pulokas, J. Quispe, C. Suloway, Y. Zhu and C.S. Potter. (2004) "Rapid, routine structure determination of macromolecular assemblies using electron microscopy: Current progress and remaining challenges", J. Synchrotron Rad. 11, 83-85.

Zhu, Yuanxin, Bridget Carragher, Fabrice Mouche, and Clinton S. Potter. (2003) Automatic Particle Detection through Efficient Hough Transforms, IEEE Transactions on Medical Imaging, 22,1053-1062