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Sandra Encalada, Ph.D.

Assistant Professor
Arlene and Arnold Goldstein Assistant Professor
Department of Molecular Medicine
California Campus
Laboratory Website
Scripps VIVO Scientific Profile
encalada@scripps.edu
(858) 784-9681

Scripps Research Joint Appointments

Department of Molecular Medicine
Department of Molecular and Cellular Neuroscience
Dorris Neuroscience Center
Faculty, Graduate Program

Research Focus

Intracellular Transport and Neurodegeneration

Our lab is interested in characterizing the interactions between molecular motors and their vesicular cargo to regulate axonal transport in neurons.  We use a combination of cell biology, genetics, biochemistry, and high-resolution microscopy in mammalian neurons and in the soil nematode Caenorhabditis elegans, to identify and characterize motor-cargo regulatory complexes. 

We also seek to integrate and extend our knowledge of motor regulation to dissect the role that defective vesicular transport plays in the accumulation of misfolded proteins inside axons, and scrutinize the hypothesis that this contributes to or causes neurodegeneration.  We use mammalian and C. elegans systems to build models of protein aggregation diseases including prion diseases, Alzheimer’s disease, Amyotrophic Lateral Sclerosis (ALS), tauopathies, and transthyretin (TTR) amyloidosis-related diseases, to characterize the role of motor-based transport in toxicity and infectivity.

 

Education

B.A., Physics, Earlham College, 1992
M.S., Population Genetics, University of Florida, 1995
Ph.D., Molecular Genetics, Institute of Molecular Biology, University of Oregon, 2003

Professional Experience

2013-2017 Assistant Professor (Joint Appointment), Cell and Molecular Biology (CMB), The Scripps Research Institute
2013-2017 Assistant Professor (Joint Appointment), Molecular and Cellular Neuroscience (MCN), The Scripps Research Institute
2011-2017 Arlene and Arnold Goldstein Assistant Professor, Molecular and Experimental Medicine (MEM), The Scripps Research Institute
2011-2012 Assistant Professor (Joint Appointment), Cell Biology, The Scripps Research Institute

Awards & Professional Activities

The Glenn Award for Research in Biological Mechanisms of Aging, 2015
The Baxter Foundation Young Faculty Award, 2015
The Ellison Medical Foundation New Scholar in Aging Award 2012-1016

Damon Runyon Cancer Research Foundation Postdoctoral Fellowship, 2004-2007
NIH Biophysics and Molecular Biology Pre-doctoral Training Grant, 1998-2003
Pre-doctoral Research Training Grant in Genetic Mechanisms of Evolution, 1997-1998
Phi Kappa Phi Honor Society, 1995
Sigma Xi, The Scientific Research Society, 1992
Warren Staebler Scholarship, Earlham College, 1991
Undergraduate Studies Scholarship, Earlham College, 1988-1992
International Scholarship, The Armand Hammer United World College of the American West, 1986-1988

Selected References

All Publications

Neumann S, Chassefeyre R, Campbell GE, Encalada SE. KymoAnalyzer: a software tool for the quantitative analysis of intracellular transport in neurons. Traffic. 2017 Jan;18(1):71-88. doi: 10.1111/tra.12456. PubMed PMID: 27770501.

Rangaraju, S., Solis G.M., Thompson R.C., Gomez-Amaro R.L., Kurian L., Encalada S.E., Niculescu III, A.B., Salomon D.R., Petrascheck M. Suppression of transcriptional drift extends C. elegans lifespan by postponing the onset of mortality. eLife 2015 Dec 1;4:e08833. doi: 10.7554/eLife.08833. 

Baranczak A, Liu Y, Connelly S, Dan DU, W-E; Greiner ER, Genereux JC, Wiseman RL, Eisele YS, Bradbury NC, Dong J, Noodleman L, Sharpless KB, Wilson IA, Encalada SE*, Kelly JW*. A Fluorogenic Aryl Fluorosulfate for Intraorganellar Transthyretin Imaging in Living Cells and in Caenorhabditis elegansJ. Am. Chem. Soc. 2015;137(23):7404-14. doi: 10.1021/jacs.5b03042. PubMed PMID: 26051248; NIHMSID 696951. *Co-corresponding authors.

Eisele YS, Monteiro C, Fearns C, Encalada SE, Wiseman RL, Powers ET, Kelly JW. Targeting Protein Aggregation to Ameliorate Degenerative Diseases. Nature Reviews Drug Discovery, 2015, Nov;14(11):759-80. doi:10.1038/nrd4593. Published online 4 September 2015; NIHMSID 696850.

Neumann S, Campbell GE, Szpankowski L, Goldstein LSB, Encalada SE*. Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis. JoVE, 2014 (92), October 30, e52029, doi:10.3791/52029. *Corresponding author.

Encalada SE*, Goldstein LSB*. Biophysical Challenges to Axonal Transport:  Motor-Cargo Deficiencies and Neurodegeneration.  Annual Review of Biophysics, 43 2014; 43:7.1-7.29.  doi:10.1146/annurev-biophys-051013-022746. *Co-corresponding authors.

Otero MG, Alloatti M, Cromberg LE, Almenar-Queralt A, Encalada SE, Pozo Devoto VM, Goldstein LSB, Falzone TL. Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function. J. Cell Sci. 2014, 127: 1537-1549.

Szpankowski LJ, Encalada SE, Goldstein LSB. Sub-Pixel Colocalization Reveals Amyloid Precursor Protein Dependent Kinesin-1 and Dynein Association with Axonal Vesicles. PNAS 2012, 109(22):8582-7.

Encalada SE*, Szpankowski LJ, Xia C-H, Goldstein LSB*. Stable Kinesin and Dynein Assemblies Drive the Axonal Transport of Mammalian Prion Protein Vesicles. Cell 2011, 144: 551–565. *Co-corresponding authors.

Encalada SE, Goldstein LSB. Axonal Transport of the Prion Protein and Prion Neuroinvasion. In: The New Encyclopedia of Neuroscience (Larry Squire, ed.). Elsevier, Oxford, UK, 2009, pp. 1071-1075

Encalada SE, Moya KL, Lehmann S, Zahn R. The role of the prion protein in the molecular basis for synaptic plasticity and nervous system development. Journal of Molecular Neuroscience 2008, 34(1):9-15. Epub 2007 Jun 14.

Encalada, SE, Willis J, Lyczak R, Bowerman B. A Spindle Checkpoint Functions During Mitosis in the Early C. elegans Embryo. Molecular Biology of the Cell, 2005, 16: 1056-1070.

Gomes J-E, Encalada SE, Swan KA, Shelton CA, Carter JC, Bowerman B. The maternal gene spn-4 encodes a predicted RRM protein required for mitotic spindle orientation and cell fate patterning in early C. elegans embryos.  Development 2001, 128:4301-4314.

Encalada SE, Martin PR , Phillips JB, Lyczak R, Hamill DR, Swan KA, Bowerman B. DNA replication defects delay cell division and disrupt cell polarity in early Caenorhabditis elegans embryos.  Developmental Biology 2000, 228:225-238.