Faculty, Graduate Program
NMR is the method of choice in studying unfolded proteins, as well as proteins such as the prion protein that have highly disordered parts. Since many of the entries in the published genomes appear to code for proteins that should be intrinsically unstructured, an understanding of the nature and behavior of unfolded proteins is assuming increasing importance. Many important proteins contain disordered or dynamic regions, making structure determination by X-ray crystallography and cryo-EM difficult. Incorporation of NMR studies into the determination of the structure and function of molecular machines can define not only structures of flexible portions, but can give insights into the role of molecular motions and disorder into the function of the machine. We have used such a combined approach to examine the complexes of the transcriptiion factor NF-kappaB with DNA and with its inhibitor IkappaB. THese studies have led to new insights into the mechanisms by which NF-kappaB transcriptional activation is turned on and off. Another major emphasis in the lab is on the interactions of chaperones with their client proteins and co-chaperones. Again a combined approach using NMR gives new information on these dynamic and heterogeneous systems.
B.S., Biochemistry, University of Sydney, 1973
D.Sc., Faculty of Science, University of Sydney, 2009
Ph.D., Inorganic Chemistry, University of Sydney, 1977
1977-1978 Postdoctoral Fellow, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA (supervisor Paul Schimmel).
1979-1984 UNESCO Lecturer, School of Chemistry, University of New South Wales Australia.
1984-1988 Research Associate, Department of Molecular Biology, The Scripps Research Institute.
1988-1992 Assistant Member, Department of Molecular Biology, The Scripps Research Institute.
1992-1997 Associate Professor, Department of Molecular Biology, The Scripps Research Institute.
1997-2001 Associate Professor with Tenure, Department of Molecular Biology, The Scripps Research Institute
2001-2013 Professor, Department of Molecular Biology, The Scripps Research Institute
2013-present Professor, Department of Integrative Structural and Computational Biology, The Scripps Research Institute
1971 Roslyn Flora Goulston Prize for Biochemistry, Faculty of Science, University of Sydney.
1977-1978 Postdoctoral Award, Damon Runyon-Walter Winchell Cancer Fund.
2009 Awarded D.Sc., Faculty of Science, University of Sydney.
2010 Distinguished Scientist Award, San Diego Section of the American Chemical Society.
2017-2021 Editor-in-Chief, Biophysical Journal
The client protein p53 forms a molten globule-like state in the presence of Hsp90. S.J. Park, M.A. Martinez-Yamout and H.J. Dyson (2011) Nature Struct. Mol. Biol. 18, 537-542.
Detection of a ternary complex of NFkB and IkBa with DNA provides insights into how IkBa removes NFkB from transcription sites. S.C. Sue, V. Alverdi, E.A. Komives and H.J. Dyson (2011) Proc. Natl. Acad. Sci. USA 108.1367-1372
Expanding the proteome: disordered and alternatively folded proteins. H.J. Dyson (2011) Quart. Rev. Biophysics 44, 467-518.
Intrinsically disordered proteins in cellular signalling and regulation. P.E. Wright and H.J. Dyson (2015) Nat. Rev. Mol. Cell Biol. 16, 18-29.
Structural characterization of the ternary complex that mediates termination of NF-kB signaling by IkBa. S.P. Mukherjee, P.O. Quintas, R. McNulty, E.A. Komives and H.J. Dyson (2016) Proc. Natl. Acad. Sci. USA 113, 6212-6217.