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Bridget Carragher, Ph.D.

Professor of Cell Biology
Department of Integrative Structural and Computational Biology
California Campus
Laboratory Website
bcarr@scripps.edu
(858) 784-9070

Research Focus

Investigating the dynamics of molecular machines using automated electron microscopy

The focus of our group is the development of automated imaging techniques for enabling the investigation of the structure, function and dynamics of molecular machines.

Professional Experience

B.Sc., Physics, University of the Witwatersrand, 1977   
B.Sc. Hons, Physics, University of the Witwatersrand, 1978
M.Sc., Physics, Northwestern University, 1982   
Ph.D., Biophysics, University of Chicago, 1987

Awards & Professional Activities

Associate Editor, Journal of Structural Biology
Director, National Resource for Automated Molecular Microscopy

Selected References

Lyumkis D, Doamekpor SK, Bengtson MH, Lee JW, Toro TB, Petroski MD, Lima CD, Potter CS, Carragher B, Joazeiro CA. Single-particle EM reveals extensive conformational variability of the Ltn1 E3 ligase. Proc Natl Acad Sci U S A. 2013;110(5):1702-7. PMCID: PMC3562785.

Tao H, Lee SC, Moeller A, Roy RS, Siu FY, Zimmermann J, Stevens RC, Potter CS, Carragher B, Zhang Q. Engineered nanostructured [beta]-sheet peptides protect membrane proteins. Nat Meth. 2013;10(8):759-61.

Lander GC, Johnson JE, Rau DC, Potter CS, Carragher B, Evilevitch A. DNA bending-induced phase transition of encapsidated genome in phage lambda. Nucleic Acids Res. 2013;41(8):4518-24. PMCID: PMC3632124.

Veesler D, Campbell MG, Cheng A, Fu CY, Murez Z, Johnson JE, Potter CS, Carragher B. Maximizing the potential of electron cryomicroscopy data collected using direct detectors. J Struct Biol. 2013.

Yoshioka C, Lyumkis D, Carragher B, Potter CS. Maskiton: Interactive, web-based classification of single-particle electron microscopy images. J Struct Biol. 2013;182(2):155-63. PMCID: PMC3645979.

Moeller, A, Kirchdoerfer RN, Potter CS, Carragher B, Wilson IA. Organization of the influenza virus replication machinery. Science. 2012;338(6114):1631-4.

Lyumkis D, Doamekpor SK, Bengtson MH, Lee JW, Toro TB, Petroski MD, Lima CD, Potter CS, Carragher B, Joazeiro CA. Single-particle EM reveals extensive conformational variability of the Ltn1 E3 ligase. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(5):1702-7. PMCID: 3562785.

Lau PW, Guiley KZ, De N, Potter CS, Carragher B, MacRae IJ. The molecular architecture of human Dicer. Nat Struct Mol Biol. 2012;19(4):436-40. PMCID: PMC3319852.

Campbell MG, Cheng A, Brilot AF, Moeller A, Lyumkis D, Veesler D, Pan J, Harrison SC, Potter CS, Carragher B, Grigorieff N. Movies of ice-embedded particles enhance resolution in electron cryo-microscopy. Structure. 2012;20(11):1823-8. PMCID: 3510009.

Veesler D, Quispe J, Grigorieff N, Potter CS, Carragher B, Johnson JE. Maturation in action: CryoEM study of a viral capsid caught during expansion. Structure. 2012;20(8):1384-90. PMCID: PMC3418467.

Moeller A, Zhao C, Fried MG, Wilson-Kubalek EM, Carragher B, Whiteheart SW. Nucleotide-dependent conformational changes in the N-Ethylmaleimide Sensitive Factor (NSF) and their potential role in SNARE complex disassembly. J Struct Biol. 2012;177(2):335-43. PMCID: PMC3382979.

Lau PW, Potter CS, Carragher B, Macrae IJ. DOLORS: Versatile Strategy for Internal Labeling and Domain Localization in Electron Microscopy. Structure. 2012;20(12):1995-2002. PMCID: 3535077.

Yoshioka C, Carragher B, Potter CS. Cryomesh: a new substrate for cryo-electron microscopy. Microsc Microanal. 2010;16(1):43-53. PMCID: PMC2840046.

Voss NR, Lyumkis D, Cheng A, Lau PW, Mulder A, Lander GC, Brignole EJ, Fellmann D, Irving C, Jacovetty EL, Leung A, Pulokas J, Quispe JD, Winkler H, Yoshioka C, Carragher B, Potter CS. A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy. J Struct Biol. 2010;169(3):389-98. PMCID: PMC2826578.

Lyumkis D, Moeller A, Cheng A, Herold A, Hou E, Irving C, Jacovetty EL, Lau PW, Mulder AM, Pulokas J, Quispe JD, Voss NR, Potter CS, Carragher B. Automation in single-particle electron microscopy connecting the pieces. Methods Enzymol. 2010;483:291-338.Voss NR, Yoshioka CK, Radermacher M, Potter CS, Carragher B. DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy. J Struct Biol. 2009;166(2):205-13. PMCID: PMC2768396.

Mulder AM, Glavis-Bloom A, Moores CA, Wagenbach M, Carragher B, Wordeman L, Milligan RA. A new model for binding of kinesin 13 to curved microtubule protofilaments. J Cell Biol. 2009;185(1):51-7. PMCID: PMC2700504.

Lander GC, Tang L, Casjens SR, Gilcrease EB, Prevelige P, Poliakov A, Potter CS, Carragher B, Johnson JE. The structure of an infectious P22 virion shows the signal for headful DNA packaging. Science. 2006;312(5781):1791-5.

Mulder AM, Yoshioka C, Beck AH, Bunner AE, Milligan RA, Potter CS, Carragher B, Williamson JR. Visualizing ribosome biogenesis: parallel assembly pathways for the 30S subunit. Science. 2010;330(6004):673-7. PMCID: PMC2990404.

Stagg SM, LaPointe P, Razvi A, Gurkan C, Potter CS, Carragher B, Balch WE. Structural basis for cargo regulation of COPII coat assembly. Cell. 2008;134(3):474-84. PMCID: PMC2649882.

Links

NRAMM website

AMI group website