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Faculty
John Yates III
Professor
CHEMICAL PHYSIOLOGY
TSRI - 2000
Education
B.A., University of Maine, 1980, Zoology
M.S., University of Maine, 1983, Chemistry
Ph.D., University of Virginia, 1987, Chemistry
Awards & Activities
Associate Editor, Analytical Chemistry
Board of Reviewing Editors Science
Biemann Award 2004
American Society for Mass Spectrometry Research Award 1996
Pehr Edman Award 1998
Research Focus
Genomic and EST sequencing projects are providing a sequence infrastructure that is changing how protein biochemistry is practiced. These data along with mass spectrometry provide the cornerstone technologies fueling the proteomics revolution. Our group is focused on developing tools and strategies in proteomics to answer basic biological questions. By coupling the additional amino acid sequence information that can be obtained using peptide tandem mass spectrometry to "back-end" database searching and "front-end" separative techniques, we are able to directly analyze extremely complex protein mixtures. The applications for these technologies vary from analyzing purified protein complexes to studying host-pathogen interactions in diseases such as malaria or anthrax. We are further extending these applications to find post-translational modifications to the proteins within these same complex mixtures.
Selected References
Skop, A. R., Liu, H., Yates, J. R., Meyer, B. J., Heald, R. Functional proteomic analysis of the mammalian midbody reveals conserved cell division components, Science 305, 61-6. 2004
Eric C. Schrimer, Laurence Florens, Tinglu Guan, John R. Yates III, and Larry Gerace Novel Membrane Proteins With Potential Disease Links Found By Subtractive Proteomics, Science 301, 1380-1382. 2003
Christine C. Wu, Michael J. MacCoss, Kathryn E. Howell, John R. Yates, III A novel method for the comprehensive proteomic analysis of membrane proteins: Identification, Modifications, and Topology, Nature Biotechnology 21, 262-7. 2003
Boddy, M.N., Gaillard, P.H.L., McDonald, W.H., Shanahan, P., Yates, J.R., 3rd, Russell, P. Mus81-Eme1 are essential components of a Holliday junction resolvase. Cell 107, 537-548. 2001
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