Paulson Laboratory

Juliesta E. Sylvester, Ph.D.
 
The Scripps Research Institute
10550 North Torrey Pines Road, MEML71
La Jolla, CA 92037
858-784-9636(phone)
858-784-9690 (FAX)
jsylves@scripps.edu

EDUCATION:

June 2010 - Ph. D. Biochemistry and molecular biology, University of Chicago (USA)

2000 - B.A. Biological sciences, University of Chicago (USA)



PROFESSIONAL EXPERIENCES:

Jan 2013 to Present - Research Associate, The Scripps Research Institute, La Jolla, CA, USA (Advisor: Prof. James C. Paulson)

2011 to 2012 - Post-doctoral, Massachusetts Institute of Technology, Boston, MA, USA (Advisors: Prof. Douglas Lauffenburger; Frank Gertler; Michael Hemann)

2010 to 2011 - Post-doctoral, University of Chicago, IL, USA (Advisor: Prof. Stephen J. Kron)

2006 to 2010 - Doctoral Candidate, University of Chicago, IL, USA (Advisor: Prof. Stephen J. Kron)

2005 - Research Collaboration, University of Wisconsin, Madison, WI, USA (Collaborator: Sean Palacek)

2003 to 2004 - Research Collaboration, Argonne National Laboratory, IL, USA (Collaborators: Prof. Andrzej Joachimiak; Carol Giometti; Gyorgy Babnigg)

2002 to 2006 - Research Assistant, University of Chicago, IL, USA (Advisor: Prof. Anthony A. Kossiakoff)

2001 to 2002 - Research Assistant, University of Chicago, IL, USA (Advisors: Prof. Deborah Nelson; Prof. Kamal Sharma)

2000 - Research Assistant, University of Chicago, IL, USA (Advisor: Prof. Daphne Preuss)

1999 to 2000 - Research Assistant, University of Chicago, IL, USA (Advisor: Prof. Philip Ulinski)

1999 - Research Assistant, University College London, UK (Advisor: Prof. Stephen W. Davies)

1999 - Research Assistant, Department of Public Health, Los Angles, CA, USA



INDUSTRIAL COLLABORATIONS:

2012 - Consultant and Analyst, Beacon Value Partners, MA, USA

2011 to 2012 - Post-doctoral research associate, Janssen Pharmaceuticals Inc., NJ, USA

2010 to 2011 - Research Collaborator, Millipore Corporation, CA, USA

2008 to Present - Chief Science Officer, Biophysics Research Group, Inc., CA, USA

2007 to present - Collaborator and adjunct representative, Luminex Corporation, TX, USA

2007 to present - Research Collaborator, Solulink Biosciences, CA, USA


ACADEMIC AND PROFESSIONAL HONORS:

2007-2009: Graduate Fellowship, American Heart Association, Midwest Affiliate.

2003: Graduate Training Grant, Department of Neuroscience, University of Chicago.

1999-2000: Honors Curriculum, University of Chicago.

1999-2000: Berkman Undergraduate Scholarship, University of Chicago.

1997: Dean's Honors List, Universtity of Chicago.

1996-1998: Berkman Undergraduate Scholarship, University of Chicago.

1996: Dean's Honors List, Universtity of Chicago.

1996: Certificat d'Honneur, Concours National de Francais, Niveau 5.



PATENTS:

Method for quantitative analysis of proteomic data, No. 13068026.
T.S. Bray, J.E. Sylvester, S.J. Kron. Filed May 2, 2011 (Status: pending)

Method for accurate measurement of enzyme activities, No. 12660608.
J.E. Sylvester and S.J. Kron. Filed March 2, 2010. (Status: pending)


PUBLICATIONS:

Zhou, G., Khan, F., Dai, Q., Sylvester, J.E. & Kron, S.J. Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS. Mol Biosyst 8, 2395-2404 (2012).

Sylvester, J.E., Bray, T.S. & Kron, S.J. Annotator: postprocessing software for generating function-based signatures from quantitative mass spectrometry. J Proteome Res 11, 1521-1536 (2012).

Zhou, G., Sylvester, J.E., Wu, D., Veach, D.R. & Kron, S.J. A magnetic bead-based protein kinase assay with dual detection techniques. Anal Biochem 408, 5-11 (2011).

Wu, D., Sylvester, J.E., Parker, L.L., Zhou, G. & Kron, S.J. Peptide reporters of kinase activity in whole cell lysates. Biopolymers 94, 475-486 (2010).

Sylvester, J.E. & Kron, S.J. A bead-based activity screen for small-molecule inhibitors of signal transduction in chronic myelogenous leukemia cells. Mol Cancer Ther 9, 1469-1481 (2010).

Walsh, S.T., Sylvester, J.E. & Kossiakoff, A.A. The high- and low-affinity receptor binding sites of growth hormone are allosterically coupled. Proc Natl Acad Sci U S A 101, 17078-17083 (2004).

Colombe, J.B., Sylvester, J., Block, J. & Ulinski, P.S. Subpial and stellate cells: two populations of interneurons in turtle visual cortex. J Comp Neurol 471, 333-351 (2004).

Walsh, S.T., Jevitts, L.M., Sylvester, J.E. & Kossiakoff, A.A. Site2 binding energetics of the regulatory step of growth hormone-induced receptor homodimerization. Protein Sci 12, 1960-1970 (2003).


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