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Ollmann Saphire Laboratory


2017
Bruhn, J.F., Kirchdoerfer, R.N., Urata, S.M., Tickle, I.J., Bricogne, G., Saphire, E.O. (2017) Crystal Structure of the Marburg Virus VP35 Oligomerization Domain. J. Virol. Jan 3;91(2).
2016
Hastie, K.M., Igonet, S., Sullivan, B.M., Legrand, P., Zandonatti, M.A., Robinson, J.E., Garry, R.F., Rey, F.A., Oldstone, M.B., and Saphire E.O. (2016) Crystal structure of the prefusion surface glycoprotein of the prototypic arenavirus LCMV. Nat. Struct. Mol. Biol., in press.

Hastie, K.M., Zandonatti, M.A., Liu, T., Li, S., Woods, V.L., Jr., Saphire, E.O. (2016) Crystal structure of the oligomeric form of the Lassa virus matrix protein Z. J. Virol. In press.

Bornholdt, Z.A., Ndungo, E., Fusco, M.L., Flyak, A.I., Crowe, J.E., Chandran, K., and Saphire, E.O. (2016) Host-primed Ebola virus GP exposes a hydrophobic NPC1 receptor-binding pocket, revealing a target for broadly neutralizing antibodies. mBio, e02154-15.

Howell, K.E., Qiu, X., Brannan, J.M., Bryan, C., Davidson, E., Holtsberg, F.W., Wec, A.Z., Shulenin, S., Biggins, J.E., Douglas, R., Enterlein, S.G., Turner, H.L., Pallesen, J., Murin, C.D., He, S., Kroeker, A., Vu, H., Herbert, A.S., Fusco, M.L., Nyakatura, E.K., Lai, J.K., Keck, Z.-Y., Foung, S.K.H., Saphire, E.O., Zeitlin, L., Ward, A.B., Chandran, K., Doranz, B.J., Kobinger, G.P., Dye, J.M., Aman, M.J. (2016) Antibody treatment of Ebola and Sudan virus infection via a uniquely exposed epitope within the glycoprotein receptor-binding site. Cell Reports, in press.

Flyak, A.I., Shen, X., Murin, C.D., Turner, H.L., David, J.A., Fusco, M.L., Lampley, R., Kose, N., Ilinykh, P.A., Kuzmina, N., Branchizio, A., King, H., Brown, L., Bryan, C., Davudson, E., Doranz, B.J., Slaughter, J.C., Sapparapu, G., Klages, C., Ksiazek, T.G., Saphire, E.O., Ward, A.B., Bukreyev, A., and Crowe, J.E., Jr. (2016) Cross-Reactive and Potent Neutralizing Antibody Responses in Human Survivors of Natural Ebolavirus Infection. Cell. 164:1-14.

Bornholdt ZA, Turner HL, Murin CD, Li W, Sok D, Souders CA, Piper AE, Goff A, Shamblin JD, Wollen SE, Sprague TR, Fusco ML, Pommert KB, Cavacini LA, Smith HL, Klempner M, Reimann KA, Krauland E, Gerngross TU, Wittrup KD, Saphire EO, Burton DR, Glass PJ, Ward AB, Walker LM. (2016) Isolation f potent neutralizing anitbodies form a survivor of the 2014 Ebola virus outbreak. Science. 351:1078-83

Oda, S., Halfmann, P., Noda, T., Wijesinghe, K., Bornholdt, Z.A., Abelson, D.M., Armbrust, T., Stahelin, R.V., Kawaoka, Y., and Saphire, E.O. (2016) Crystal structure of Marburg virus VP40: matrix assembly and immunosuppression. J. Virol., 90:1829-48.

Qiu X, Audet J, Lv M, He S, Wong G, Wei H, Luo L, Fernando L, Kroeker A, Bovendo HF, Bello A, Li F, Ye P, Jacobs M, Ippolito G, Saphire EO, Bi S, Shen B, Gao GF, Zeitlin L, Feng J, Zhang B, Kobinger GP. (2016) Two mAb cocotail protects maqaques against the Makona variant of Ebola virus. Sci. Transl. Med. 8:329ra33


2015
Kirchdoerfer, R.N., Abelson, D.M., Li, S., Wood, M.R., and Saphire, E.O. (2015) Assembly of the Ebola virus Nucleocapsid from a chaperoned VP35 complex. Cell Reports pii: S2211-1247(15)00586-0. 12:140-9.
Fusco, M.L., Hashiguchi, T., Cassan, R., Biggins, J.E., Murin, C.D., Warfield, K.L., Li, S., Holtsberg, F.W., Shulenin, S., Vu, H., Olinger, G.G., Kim, D.H., Whaley, K.J., Zeitlin, L., Ward, A.B., Nykiforuk, C., Aman, M.J., Berry, J., and Saphire, E.O. (2015) Protective mAbs and Cross-Reactive mAbs Raised by Immunization with Engineered Marburg virus GPs. PloS Pathog. 11(6):e1005016.
Description: Macintosh HD:Users:erica:Desktop:cov150h.gifBoisen, M.L., Oottamasathein, D., Jones, A.B., Millett, M.M., Nelson, D.S., Bornholdt, Z.A., Fusco, M.L., Abelson, D.M., Oda, S., Hartnett, J.N., Roland, M.M., Heinrich, M.L., Akdag, M., Goba, A., Momoh, M., Fullah, M., Baimba, F., Gbakie, M., Safa, S., Fonnie, R., Kanneh, L., Cross, R.W., Geisbert, J.B., Geisbert, T.W., Kulakofsky, P.C., Grant, D.S., Shaffer, J.G., Scheiffelin, J.S., Wilson, R.B., Saphire, E.O., Branco, L.M., Garry, R.F., Khan, S.H., and Pitts, K.R. for the Viral Hemorrhagic Fever Consortium. (2015) Development of Prototype Filovirus Recombinant Antigen Immunoassays. J. Infect. Dis., 212: Suppl 2:S359-67.
Clifton, M.C., Bruhn, J.F., Atkins, K., Webb, T.L., Baydo, R.O., Raymond, A., Lorimer, D.D., Edwards, T.E., Myler, P.J., and Saphire, E.O. (2015) High-resolution Crystal Structure of Dimeric VP40 From Sudan ebolavirus. J Infect Dis. May 9. pii: jiv090
Hashiguchi, T., Fusco, M.L., Bornholdt, Z.A., Lee, J.E., Flyak, A., Matsuoka, R., Kohda, D., Yanagi, Y., Hammel, M., Crowe, J.E., and Saphire, E.O. (2015) Structural basis for Marburg virus neutralization by a cross-reactive human antibody. Cell, 160:904-12.
Flyak, A., Ilinykh, P.A., Murin, C.D., Garron, T., Shen, X., Fusco, M.L., Hashiguchi, T., Bornholdt, Z.A., Slaughter, J.A., Sapparapu, G., Klages, C. Ksiazek, T.G., Ward, A.B., Saphire, E.O., Bukreyev, A., and Crowe, J.E. (2015) Mechanism of Human Antibody-Mediated Neutralization of Marburg virus. Cell, 160:893-903.
Holtsberg, F.W., Shulenin, S., Vu, H., Howell KA, Patel SJ, Gunn B, Karim M, Lai JR, Frei JC, Nyakatura EK, Zeitlin L, Douglas R, Fusco ML, Froude JW, Saphire EO, Herbert AS, Wirchnianski AS, Lear-Rooney CM, Alter G, Dye JM, Glass PJ, Warfield KL, Aman MJ. (2015) Pan-ebolavirus and pan-filovirus mouse monoclonal antibodies: protection against Ebola and Sudan viruses. J. Virol. 90:266-278.
Boisen, M., Schieffelin, J.S., Goba, A., Oottamasathein, D., Jones, A.B., Shaffer, J.G., Hastie, K.M., Hartnett, J.N., Momoh, M., Fullah, M., Gabiki, M., Safa, S., Zanodnatti, M., Fusco, M., Bornholdt, Z., Abelson, D., Gire, S.K., Andersen, K.G., Tariyal, R., Stremlau, M., cross, R.W., Geisbert, J.B., Pitts, K.R., Geisbert, T.W., Kulakoski, P., Wilson, R.B., Henderson, L., Sabeti, P.C., Grant, D.S., Garry, R.F., Saphire, E.O., Branco, L.M., and Khan, S.H. (2015) Multiple circulating infections can mimic the early stages of viral hemorrhagic fevers and possible human exposure to filoviruses in Sierra Leone prior to the 2014 outbreak. Viral Immunol. 28:19-31.


2014
Structure of protective antibodies reveal sites of vulnerability on Ebola Virus.  Murin CD, Fusco, ML, Bornholdt ZA, Qiu X, Olinger GG, Zeitlin L, Kobinger GP, Ward AB, Saphire EO. 2014. Proc Natl Acad Sci U S A Nov. 17.
Structure of the LCMV nucleoprotein provides a template for understanding arenavirus replication and immunosuppression. West BR, Hastie KM, Saphire EO. 2014.  Acta Crystallography D. June; 70(Pt 6): 1764-9.
Crystal structure of Marburg virus VP24. Zhang AP, Bornholdt ZA, Abelson DM, and Saphire EO. 2014. J Virol. 88 (10):5859-63.
Crystal structure of the nipah virus phosphoprotein tetramerization domain. Bruhn JF, Barnett KC, Bibby J, Thomas JM, Keegan RM, Rigden DJ, Bornholdt ZA, and Saphire EO. 2014.  J Virol. 88:758-762.
Structure of the Reston ebolavirus VP30 C-terminal domain. Clifton MC, Kirchdoerfer RN, Atkins K, Abendroth J, Raymond A, Grice R, Barnes S, Moen S, Lorimer D, Edwards TE, Myler PJ, Saphire EO. 2014. Acta Crystallography F. Apr; 70(Pt 4): 457-60.
Lassa Fever in post-conflict Sierra Leone. Shaffer JG, Grant DS, Schieffelin JS, Boisen ML, Goba A, Hartnett JN, Levy DC, Yenni RE, Moses LM, Fullah M, Momoh M, Fonnie M, Fonnie R, Kanneh L, Koroma VJ, Kargbo K, Ottomassathien D, Muncy IJ, Jones AB, Illick MM, Kulakosky PC, Haislip AM, Bishop CM, Elliot DH, Brown BL, Zhu H, Hastie KM, Andersen KG, Gire SK, Tabrizi S, Tariyal R, Stremlau M, Matschiner A, Sampey DB, Spence JS, Cross RW, Geisbert JB, Folarin OA, Happi CT, Pitts KR, Geske FJ, Geisbert TW,Saphire EO, Robinson JE, Wilson RB, Sabeti PC, Henderson LA, Khan SH, Bausch DG, Branco LM, Garry RF; Viral Hemorrhagic Fever Consortium. 2014. PLoS Negl Trop Dis. 8:e2748.
Complement is activated by IgG hexamers assembled at the cell surface. Diebolder CA, Beurskens FJ, de Jong RN, Koning RI, Strumane K, Lindorfer MA, Voorhorst M, Ugurlar D, Rosati S, Heck AJ, van de Winkel JG, Wilson IA, Koster AJ, Taylor RP, Saphire EO, Burton DR, Schuurman J, Gros P, and Parren PW. 2014.  Science. 343:1260-1263.
2013
An update on the use of antibodies against the filoviruses. Saphire, E. O. 2013. Immunotherapy 5:1221-1233.
Discussions and decisions of the 2012-2014 International Committee on Taxonomy of Viruses (ICTV) Filoviridae Study Group. Bukreyev AA, Chandran K, Dolnik O, Dye JM, Ebihara H, Leroy EM, Mühlberger E, Netesov SV, Patterson JL, Paweska JT, Saphire EO, Smither SJ, Takada A, Towner JS, Volchkov VE, Warren TK, and Kuhn JH. 2013. January 2012-June 2013. Arch Virol.
Ebolavirus VP35 coats the backbone of double-stranded RNA for interferon antagonism. Bale S, Julien JP, Bornholdt ZA, Krois AS, Wilson IA, and Saphire EO. 2013. J Virol. 87:10385-10388.
Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle. Bornholdt, Z. A., T. Noda, D. M. Abelson, P. Halfmann, M. R. Wood, Y. Kawaoka, and E. O. Saphire. 2013.  Cell 154:763-774.
Virus nomenclature below the species level: a standardized nomenclature for laboratory animal-adapted strains and variants of viruses assigned to the family Filoviridae. Kuhn JH, Bao Y, Bavari S, Becker S, Bradfute S, Brister JR, Bukreyev AA, Caì Y, Chandran K, Davey RA, Dolnik O, Dye JM, Enterlein S, Gonzalez JP, Formenty P, Freiberg AN, Hensley LE, Honko AN, Ignatyev GM, Jahrling PB, Johnson KM, Klenk HD, Kobinger G, Lackemeyer MG, Leroy EM, Lever MS, Lofts LL, Mühlberger E, Netesov SV, Olinger GG, Palacios G, Patterson JL, Paweska JT, Pitt L, Radoshitzky SR, Ryabchikova EI, Saphire EO, Shestopalov AM, Smither SJ, Sullivan NJ, Swanepoel R, Takada A, Towner JS, van der Groen G, Volchkov VE, Wahl-Jensen V, Warren TK, Warfield KL, Weidmann M, and Nichol ST. 2013. Arch Virol 158:1425-1432. PMC3669655
Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle.Bornholdt, Z. A., T. Noda, D. M. Abelson, P. Halfmann, M. R. Wood, Y. Kawaoka, and E. O. Saphire. 2013. Cell 154:763-774.
The ebola virus interferon antagonist VP24 directly binds STAT1 and has a novel, pyramidal fold. Zhang, A. P., Z. A. Bornholdt, T. Liu, D. M. Abelson, D. E. Lee, S. Li, V. L. Woods, Jr., and E. O. Saphire. 2013. PLoS Pathog. Dec; 9(12) PMC3285596

2012
The ebolavirus VP24 interferon antagonist: know your enemy. Zhang, A. P., D. M. Abelson, Z. A. Bornholdt, T. Liu, V. L. Woods, Jr., and E. O. Saphire. 2012.  Virulence Aug 15;3(5):440-445.
Two synthetic antibodies that recognize and neutralize distinct proteolytic forms of the ebola virus envelope glycoprotein. Koellhoffer, J. F., G. Chen, R. G. Sandesara, S. Bale, E. O. Saphire, K. Chandran, S. S. Sidhu, and J. R. Lai. 2012. Chembiochem 13:2549-2557. PMC3684266
Structural Basis for the dsRNA Specificity of the Lassa Virus NP Exonuclease. Hastie, K. M., L. B. King, M. A. Zandonatti, and E. O. Saphire. 2012. PLoS One 7:e44211. PMC3429428
Hiding the evidence: two strategies for innate immune evasion by hemorrhagic fever viruses. Hastie, K. M., S. Bale, C. R. Kimberlin, and E. O. Saphire. 2012. Curr Opin Virol 2:151-156.
Cathepsin cleavage potentiates the Ebola virus glycoprotein to undergo a subsequent fusion-relevant conformational change. Brecher, M., K. L. Schornberg, S. E. Delos, M. L. Fusco, E. O. Saphire, and J. M. White. 2012. J Virol 86:364-372. PMC3255896
Marburg virus VP35 can both fully coat the backbone and cap the ends of dsRNA for interferon antagonism. Bale, S., J. P. Julien, Z. A. Bornholdt, C. R. Kimberlin, P. Halfmann, M. A. Zandonatti, J. Kunert, G. J. Kroon, Y. Kawaoka, I. J. MacRae, I. A. Wilson, and E. O. Saphire. 2012. PLoS Pathog 8:e1002916. PMC3441732
Structural basis for differential neutralization of ebolaviruses. Bale, S., J. M. Dias, M. L. Fusco, T. Hashiguchi, A. C. Wong, T. Liu, A. I. Keuhne, S. Li, V. L. Woods, Jr., K. Chandran, J. M. Dye, and E. O. Saphire. 2012. Viruses 4:447-470. PMC3347318

2011
Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding. Hastie, K. M., T. Liu, S. Li, L. B. King, N. Ngo, M. A. Zandonatti, V. L. Woods, Jr., J. C. de la Torre, and E. O. Saphire. 2011. Proc Natl Acad Sci U S A 108:19365-19370. PMC3228486
Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression. Hastie, K. M., C. R. Kimberlin, M. A. Zandonatti, I. J. Macrae, and E. O. Saphire. 2011.  Proc Natl Acad Sci U S A. Feb 8; 108 (6):2396-401.
A shared structural solution for neutralizing ebolaviruses. Dias, J. M., A. I. Kuehne, D. M. Abelson, S. Bale, A. C. Wong, P. Halfmann, M. A. Muhammad, M. L. Fusco, S. E. Zak, E. Kang, Y. Kawaoka, K. Chandran, J. M. Dye, and E. O. Saphire. 2011. Nat Struct Mol Biol 18:1424-1427. PMC3230659
Ebola virus glycoprotein needs an additional trigger, beyond proteolytic priming for membrane fusion. Bale, S., T. Liu, S. Li, Y. Wang, D. Abelson, M. Fusco, V. L. Woods, Jr., and E. O. Saphire. 2011. PLoS Negl Trop Dis 5:e1395. PMC3216919

2010
Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression. Kimberlin, C. R., Z. A. Bornholdt, S. Li, V. L. Woods, Jr., I. J. MacRae, and E. O. Saphire. 2010.  Proc Natl Acad Sci U S A 107:314-319. PMC2806767

2009
Ebolavirus glycoprotein structure and mechanism of entry. Lee, J. E., and E. O. Saphire. 2009. Future Virol 4:621-635. PMC2829775
Neutralizing ebolavirus: structural insights into the envelope glycoprotein and antibodies targeted against it. Lee, J. E., and E. O. Saphire. 2009. Curr Opin Struct Biol 19:408-417. PMC2759674
An efficient platform for screening expression and crystallization of glycoproteins produced in human cells. Lee, J. E., M. L. Fusco, and E. O. Saphire. 2009. Nat Protoc 4:592-604. PMC2911120
Techniques and tactics used in determining the structure of the trimeric ebolavirus glycoprotein. Lee, J. E., M. L. Fusco, D. M. Abelson, A. J. Hessell, D. R. Burton, and E. O. Saphire. 2009. Acta Crystallogr D Biol Crystallogr 65:1162-1180. PMC2777170

2008
Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain. Lee, J. E., A. Kuehne, D. M. Abelson, M. L. Fusco, M. K. Hart, and E. O. Saphire. 2008.  J Mol Biol 375:202-216.
Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor. Lee, J. E., M. L. Fusco, A. J. Hessell, W. B. Oswald, D. R. Burton, and E. O. Saphire. 2008. Nature 454:177-182.

2007
Structure of a high-affinity "mimotope" peptide bound to HIV-1-neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodies. Saphire, E. O., M. Montero, A. Menendez, N. E. van Houten, M. B. Irving, R. Pantophlet, M. B. Zwick, P. W. Parren, D. R. Burton, J. K. Scott, and I. A. Wilson. 2007.  J Mol Biol 369:696-709.

2006
Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling. Cardenas, W. B., Y. M. Loo, M. Gale, Jr., A. L. Hartman, C. R. Kimberlin, L. Martinez-Sobrido, E. O. Saphire, and C. F. Basler. 2006. J Virol 80:5168-5178.

2004
gp41: HIV's shy protein. Zwick, M. B., E. O. Saphire, and D. R. Burton. 2004. Nat Med 10:133-134.

2003
Molecular features of the broadly neutralizing immunoglobulin G1 b12 required for recognition of human immunodeficiency virus type 1 gp120. Zwick, M. B., P. W. Parren, E. O. Saphire, S. Church, M. Wang, J. K. Scott, P. E. Dawson, I. A. Wilson, and D. R. Burton. 2003. J Virol 77:5863-5876. 154005
The carbohydrate epitope of the neutralizing anti-HIV-1 antibody 2G12. Scanlan, C. N., R. Pantophlet, M. R. Wormald, E. O. Saphire, D. Calarese, R. Stanfield, I. A. Wilson, H. Katinger, R. A. Dwek, D. R. Burton, and P. M. Rudd. 2003. Adv Exp Med Biol 535:205-218.
Crystal structure of an intact human IgG: antibody asymmetry, flexibility, and a guide for HIV-1 vaccine design. Saphire, E. O., R. L. Stanfield, M. D. Crispin, G. Morris, M. B. Zwick, R. A. Pantophlet, P. W. Parren, P. M. Rudd, R. A. Dwek, D. R. Burton, and I. A. Wilson. 2003. Adv Exp Med Biol 535:55-66.
Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human immunodeficiency virus type 1 gp120. Pantophlet, R., E. O. Saphire, P. Poignard, P. W. Parren, I. A. Wilson, and D. R. Burton. 2003. J Virol 77:642-658. 140633
2002
Contrasting IgG structures reveal extreme asymmetry and flexibility. Saphire, E. O., R. L. Stanfield, M. D. Crispin, P. W. Parren, P. M. Rudd, R. A. Dwek, D. R. Burton, and I. A. Wilson. 2002. J Mol Biol 319:9-18.
Structure-based drug design. Saphire, E. O. 2002. IDrugs 5:658-661.

2001
Crystal structure of a neutralizing human IGG against HIV-1: a template for vaccine design. Saphire, E. O., P. W. Parren, R. Pantophlet, M. B. Zwick, G. M. Morris, P. M. Rudd, R. A. Dwek, R. L. Stanfield, D. R. Burton, and I. A. Wilson. 2001. Science 293:1155-1159.
Crystallization and preliminary structure determination of an intact human immunoglobulin, b12: an antibody that broadly neutralizes primary isolates of HIV-1. Saphire, E. O., P. W. Parren, C. F. Barbas, 3rd, D. R. Burton, and I. A. Wilson. 2001. Acta Crystallogr D Biol Crystallogr 57:168-171.
Listening for viral infection. Saphire, E. O., and P. W. Parren. 2001. Nat Biotechnol 19:823-824.
A model for neutralization of viruses based on antibody coating of the virion surface. Burton, D. R., E. O. Saphire, and P. W. Parren. 2001. Curr Top Microbiol Immunol 260:109-143.