Scientific Report 2008
Charles Weissmann, M.D., Ph.D.
Professor and Chairman
Corinne Lasmézas, D.V.M., Ph.D.
Donny Strosberg, Ph.D.
Tim Tellinghuisen, Ph.D.
Joaquin Castilla, Ph.D.
Senior Staff Scientist
Sukhvir Mahal, Ph.D.
Senior Research Associates
Carlos Coito, Ph.D.
Chris Baker, Ph.D.
Ivan Angulo-Herrera, Ph.D.
Shawn Browning, Ph.D.
Natalia Fernández-Borges, Ph.D.
Maria Herva-Moyana, Ph.D.
Paula Saá Prieto, Ph.D.
Jiali Li, Ph.D.
Anja Oelschlegel, Ph.D.
Yervand Karapetyan, M.D.
Minghai Zhou, Ph.D.
Distinguishing between prion strains 22L and Me7 with
the mouse bioassay takes 6 months. The cerebellar Purkinje cell layer (immunostained
for calbindin) remains intact in Me7-infected, terminally sick mice (top, arrow)
but is obliterated by infection with 22L (bottom, arrow). With the cell panel assay
(insets), the strains can be distinguished from each other in 2 weeks. 22L prions
efficiently infect the 4 cell lines constituting the panel, whereas Me7 infects
only LD9 and CAD cells. Stained sections and micrographs prepared by Sukhvir Mahal,
Ph.D., staff scientist, and Alexsandra Sherman, research assistant; photomontage
created by Christopher A. Baker, Ph.D., research associate. Work done in the laboratory
of Charles Weissmann, Ph.D.
Scripps Florida Reports
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