LOCUS       pCR2.1-U6-   4794 bp    DNA   circular             5-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376523086|
COMMENT     VNTDBDATE|382003368|
COMMENT     VNTNAME|pCR2.1-U6-GFP|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(1227..1246)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     1254..1268
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      1411..1847
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             2182..2976
                     /vntifkey="4"
                     /label=KanR
     CDS             2994..3854
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3999..4672
                     /vntifkey="21"
                     /label=pUC\ori
     primer_bind     294..310
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site\2
     primer_bind     1144..1158
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site\2
     CDS             complement(759..1048)
                     /vntifkey="4"
                     /label=U6\GFP(AS)
     CDS             complement(383..672)
                     /vntifkey="4"
                     /label=U6\GFP(S)
BASE COUNT     1174 a      1212 c      1221 g      1187 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttcaggaaa 
      301 cagctatgac catgattacg ccaagcttgg taccgagctc ggatccacta gtaacggccg 
      361 ccagtgtgct ggaattcggc ttaaaaaaaa cgatgccacc tacggcaagc ggtgtttcgt 
      421 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata 
      481 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt 
      541 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tctaattatc 
      601 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctct 
      661 tcctgcccga ccaagccgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg 
      721 catctagtaa cggccgccag tgtgctggaa ttcggcttaa aaaaaacttg ccgtaggtgg 
      781 catcgcggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca 
      841 agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc 
      901 aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa 
      961 attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat 
     1021 gggaaatagg ccctcttcct gcccgaccaa gccgaattct gcagatatcc atcacactgg 
     1081 cggccgctcg agcatgcatc tagagggccc aattcgccct atagtgagtc gtattacaat 
     1141 tcactggccg tcgttttaaa gccgaattct gcagatatcc atcacactgg cggccgctcg 
     1201 agcatgcatc tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg 
     1261 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     1321 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     1381 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc 
     1441 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     1501 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     1561 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     1621 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     1681 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     1741 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     1801 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag 
     1861 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg 
     1921 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag 
     1981 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc 
     2041 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac 
     2101 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag 
     2161 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 
     2221 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 
     2281 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 
     2341 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 
     2401 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 
     2461 ttgggcgaag tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta 
     2521 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 
     2581 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 
     2641 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 
     2701 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 
     2761 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 
     2821 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 
     2881 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 
     2941 atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta 
     3001 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 
     3061 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 
     3121 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 
     3181 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 
     3241 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 
     3301 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 
     3361 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 
     3421 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 
     3481 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 
     3541 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 
     3601 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 
     3661 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 
     3721 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 
     3781 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 
     3841 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 
     3901 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 
     3961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 
     4021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 
     4081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 
     4141 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 
     4201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 
     4261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 
     4321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 
     4381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 
     4441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 
     4501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 
     4561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 
     4621 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 
     4681 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 
     4741 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag 
//