LOCUS       pCR2.1-U6-   4797 bp    DNA   circular             5-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376519813|
COMMENT     VNTDBDATE|382003404|
COMMENT     VNTNAME|pCR2.1-U6-362|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(1230..1249)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     1257..1271
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      1414..1850
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             2185..2979
                     /vntifkey="4"
                     /label=KanR
     CDS             2997..3857
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    4002..4675
                     /vntifkey="21"
                     /label=pUC\ori
     primer_bind     294..310
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site\2
     primer_bind     1147..1161
                     /vntifkey="28"
                     /label=M13\(-20(\F\primer\binding\site\2
     CDS             complement(383..672)
                     /vntifkey="4"
                     /label=U6\LTR\362(S)
     CDS             complement(763..1051)
                     /vntifkey="4"
                     /label=U6\LTR\362(AS)
BASE COUNT     1176 a      1211 c      1221 g      1189 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttcaggaaa 
      301 cagctatgac catgattacg ccaagcttgg taccgagctc ggatccacta gtaacggccg 
      361 ccagtgtgct ggaattcggc ttaaaaaaaa gaaagtcccc agcggaaagc ggtgtttcgt 
      421 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata 
      481 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt 
      541 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tctaattatc 
      601 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctct 
      661 tcctgcccga ccaagccgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg 
      721 catctagcta gtaacggccg ccagtgtgct ggaattcggc ttaaaaaaaa ctttccgctg 
      781 gggactttcg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt 
      841 tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta 
      901 cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt 
      961 caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat 
     1021 catgggaaat aggccctctt cctgcccgac caagccgaat tctgcagata tccatcacac 
     1081 tggcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac 
     1141 aattcactgg ccgtcgtttt aaagccgaat tctgcagata tccatcacac tggcggccgc 
     1201 tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg 
     1261 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
     1321 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     1381 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc 
     1441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     1501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     1561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
     1621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     1681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     1741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     1801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa ttcagggcgc 
     1861 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc 
     1921 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag 
     1981 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca 
     2041 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta 
     2101 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa 
     2161 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 
     2221 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 
     2281 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 
     2341 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 
     2401 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 
     2461 ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa 
     2521 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 
     2581 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 
     2641 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 
     2701 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 
     2761 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 
     2821 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 
     2881 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 
     2941 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga 
     3001 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     3061 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     3121 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     3181 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     3241 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     3301 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     3361 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     3421 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     3481 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     3541 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     3601 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     3661 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     3721 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     3781 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     3841 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     3901 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     3961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     4021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     4081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     4141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     4201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     4261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     4321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     4381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     4441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     4501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     4561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     4621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     4681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     4741 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag 
//