LOCUS       pCR2.1-U6-   4218 bp    DNA   circular            10-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376519199|
COMMENT     VNTDBDATE|382468715|
COMMENT     VNTNAME|pCR2.1-U6-362(AS)|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(651..670)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     678..692
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      835..1271
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             1606..2400
                     /vntifkey="4"
                     /label=KanR
     CDS             2418..3278
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3423..4096
                     /vntifkey="21"
                     /label=pUC\ori
     promoter        complement(321..582)
                     /vntifkey="29"
                     /label=U6\promoter
     CDS             complement(294..320)
                     /vntifkey="4"
                     /label=LTR\362(AS)
BASE COUNT     1006 a      1072 c      1106 g      1034 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaaaaaaa 
      301 actttccgct ggggactttc ggtgtttcgt cctttccaca agatatataa agccaagaaa 
      361 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa 
      421 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt 
      481 gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa 
      541 tatgaaggaa tcatgggaaa taggccctct tcctgcccga ccaagccgaa ttctgcagat 
      601 atccatcaca ctggcggccg ctcgagcatg catctagagg gcccaattcg ccctatagtg 
      661 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 
      721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 
      781 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc 
      841 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
      901 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
      961 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 
     1021 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 
     1081 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
     1141 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 
     1201 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
     1261 ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt 
     1321 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa 
     1381 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac 
     1441 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag 
     1501 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc 
     1561 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 
     1621 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 
     1681 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 
     1741 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 
     1801 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 
     1861 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatcc 
     1921 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 
     1981 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 
     2041 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 
     2101 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 
     2161 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 
     2221 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 
     2281 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 
     2341 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 
     2401 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
     2461 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
     2521 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
     2581 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
     2641 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
     2701 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
     2761 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
     2821 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
     2881 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
     2941 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
     3001 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
     3061 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
     3121 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
     3181 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
     3241 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
     3301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     3361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
     3421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
     3481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 
     3541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 
     3601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 
     3661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 
     3721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 
     3781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
     3841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
     3901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
     3961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
     4021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
     4081 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
     4141 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 
     4201 tgagcgagga agcggaag 
//