LOCUS       pCR2.1-U6-   4793 bp    DNA   circular             5-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376518525|
COMMENT     VNTDBDATE|382003430|
COMMENT     VNTNAME|pCR2.1-U6-247|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(1226..1245)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     1253..1267
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      1410..1846
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             2181..2975
                     /vntifkey="4"
                     /label=KanR
     CDS             2993..3853
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3998..4671
                     /vntifkey="21"
                     /label=pUC\ori
     primer_bind     294..310
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site\2
     primer_bind     1143..1157
                     /vntifkey="28"
                     /label=M13(-20)\F\primer\binding\site\2
     CDS             complement(758..1047)
                     /vntifkey="4"
                     /label=U6-LTR\247(AS)
     CDS             complement(383..671)
                     /vntifkey="4"
                     /label=U6-LTR\247(S)
BASE COUNT     1177 a      1208 c      1218 g      1190 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttcaggaaa 
      301 cagctatgac catgattacg ccaagcttgg taccgagctc ggatccacta gtaacggccg 
      361 ccagtgtgct ggaattcggc ttaaaaaaaa caaacctcca ctctaacacg gtgtttcgtc 
      421 ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat 
      481 gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc 
      541 tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct 
      601 ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt 
      661 cctgcccgac caagccgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc 
      721 atctagtaac ggccgccagt gtgctggaat tcggcttaaa aaaaagtgtt agagtggagg 
      781 tttgcggtgt ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa 
      841 gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca 
      901 agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa 
      961 ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg 
     1021 ggaaataggc cctcttcctg cccgaccaag ccgaattctg cagatatcca tcacactggc 
     1081 ggccgctcga gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt 
     1141 cactggccgt cgttttaaag ccgaattctg cagatatcca tcacactggc ggccgctcga 
     1201 gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt 
     1261 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 
     1321 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 
     1381 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg 
     1441 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 
     1501 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 
     1561 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 
     1621 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 
     1681 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 
     1741 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 
     1801 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg 
     1861 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga 
     1921 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg 
     1981 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg 
     2041 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact 
     2101 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga 
     2161 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 
     2221 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 
     2281 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 
     2341 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 
     2401 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 
     2461 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat 
     2521 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 
     2581 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 
     2641 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 
     2701 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 
     2761 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 
     2821 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 
     2881 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 
     2941 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat 
     3001 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 
     3061 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 
     3121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 
     3181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 
     3241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 
     3301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 
     3361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 
     3421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 
     3481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 
     3541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 
     3601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 
     3661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 
     3721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 
     3781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 
     3841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 
     3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
     3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
     4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
     4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 
     4141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 
     4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 
     4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 
     4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 
     4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 
     4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 
     4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 
     4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 
     4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 
     4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 
     4741 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag 
//