LOCUS       pCR2.1-U6-   4219 bp    DNA   circular            10-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376515934|
COMMENT     VNTDBDATE|382449654|
COMMENT     VNTNAME|pCR2.1-U6-247(AS)|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(652..671)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     679..693
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      836..1272
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             1607..2401
                     /vntifkey="4"
                     /label=KanR
     CDS             2419..3279
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3424..4097
                     /vntifkey="21"
                     /label=pUC\ori
     promoter        complement(322..583)
                     /vntifkey="29"
                     /label=U6\promoter
     CDS             complement(294..321)
                     /vntifkey="4"
                     /label=LTR\247(AS)
BASE COUNT     1008 a      1067 c      1110 g      1034 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaaaaaaa 
      301 agtgttagag tggaggtttg cggtgtttcg tcctttccac aagatatata aagccaagaa 
      361 atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa 
      421 aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt 
      481 tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa 
      541 atatgaagga atcatgggaa ataggccctc ttcctgcccg accaagccga attctgcaga 
      601 tatccatcac actggcggcc gctcgagcat gcatctagag ggcccaattc gccctatagt 
      661 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
      721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
      781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc 
      841 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
      901 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
      961 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     1021 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
     1081 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     1141 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
     1201 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
     1261 attttaacaa aattcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag 
     1321 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga 
     1381 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga 
     1441 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa 
     1501 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg 
     1561 caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     1621 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     1681 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     1741 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 
     1801 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     1861 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     1921 ccaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     1981 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     2041 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     2101 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 
     2161 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 
     2221 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 
     2281 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 
     2341 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
     2401 aattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 
     2461 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 
     2521 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 
     2581 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 
     2641 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 
     2701 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 
     2761 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 
     2821 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 
     2881 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 
     2941 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 
     3001 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 
     3061 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 
     3121 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 
     3181 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 
     3241 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 
     3301 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 
     3361 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     3421 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     3481 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     3541 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 
     3601 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     3661 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     3721 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     3781 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     3841 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     3901 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     3961 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     4021 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     4081 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 
     4141 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 
     4201 gtgagcgagg aagcggaag 
//