LOCUS       pCR2.1-U6-   4801 bp    DNA   circular             5-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376521463|
COMMENT     VNTDBDATE|382003497|
COMMENT     VNTNAME|pCR2.1-U6-2161|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(1234..1253)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     1261..1275
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      1418..1854
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             2189..2983
                     /vntifkey="4"
                     /label=KanR
     CDS             3001..3861
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    4006..4679
                     /vntifkey="21"
                     /label=pUC\ori
     primer_bind     294..310
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site\2
     primer_bind     1151..1165
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site\2
     CDS             complement(765..1055)
                     /vntifkey="4"
                     /label=U6\2161(AS)
     CDS             complement(383..674)
                     /vntifkey="4"
                     /label=U6\2161\(S)
BASE COUNT     1181 a      1208 c      1218 g      1194 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttcaggaaa 
      301 cagctatgac catgattacg ccaagcttgg taccgagctc ggatccacta gtaacggccg 
      361 ccagtgtgct ggaattcggc ttaaaaaaaa gtataaacaa tgagacacca gcggtgtttc 
      421 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca 
      481 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact 
      541 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta 
      601 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct 
      661 cttcctgccc gaccaagccg aattctgcag atatccatca cactggcggc cgctcgagca 
      721 tgcatctagc tagtaacggc cgccagtgtg ctggaattcg gcttaaaaaa aactggtgtc 
      781 tcattgttta tacggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat 
      841 actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa 
      901 actacccaag aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta 
      961 cagtcaaatt aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag 
     1021 gaatcatggg aaataggccc tcttcctgcc cgaccaagcc gaattctgca gatatccatc 
     1081 acactggcgg ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta 
     1141 ttacaattca ctggccgtcg ttttaaagcc gaattctgca gatatccatc acactggcgg 
     1201 ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca 
     1261 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     1321 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
     1381 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa 
     1441 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     1501 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     1561 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     1621 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     1681 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     1741 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     1801 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaattcagg 
     1861 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg 
     1921 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag 
     1981 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac 
     2041 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa 
     2101 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga 
     2161 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 
     2221 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 
     2281 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 
     2341 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 
     2401 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 
     2461 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tcccaccttg ctcctgccga 
     2521 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 
     2581 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 
     2641 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 
     2701 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 
     2761 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 
     2821 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 
     2881 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 
     2941 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattgaaa aaggaagagt 
     3001 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 
     3061 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 
     3121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 
     3181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 
     3241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 
     3301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 
     3361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 
     3421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 
     3481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 
     3541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 
     3601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 
     3661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 
     3721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 
     3781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 
     3841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 
     3901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 
     3961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     4021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     4081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     4141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 
     4201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     4261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     4321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     4381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     4441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     4501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     4561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     4621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     4681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
     4741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
     4801 g 
//