LOCUS       pCR2.1-U6-   4221 bp    DNA   circular            10-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376520103|
COMMENT     VNTDBDATE|382468741|
COMMENT     VNTNAME|pCR2.1-U6-2161(S)|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(654..673)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     681..695
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      838..1274
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             1609..2403
                     /vntifkey="4"
                     /label=KanR
     CDS             2421..3281
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3426..4099
                     /vntifkey="21"
                     /label=pUC\ori
     promoter        complement(324..585)
                     /vntifkey="29"
                     /label=U6\promoter
     CDS             complement(294..323)
                     /vntifkey="4"
                     /label=2161\(S)
BASE COUNT     1015 a      1071 c      1105 g      1030 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaaaaaaa 
      301 agtataaaca atgagacacc agcggtgttt cgtcctttcc acaagatata taaagccaag 
      361 aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt 
      421 aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg tactaatatc 
      481 tttgtgttta cagtcaaatt aattctaatt atctctctaa cagccttgta tcgtatatgc 
      541 aaatatgaag gaatcatggg aaataggccc tcttcctgcc cgaccaagcc gaattctgca 
      601 gatatccatc acactggcgg ccgctcgagc atgcatctag agggcccaat tcgccctata 
      661 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
      721 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
      781 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc 
      841 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
      901 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
      961 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 
     1021 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
     1081 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     1141 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
     1201 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     1261 gaattttaac aaaattcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc 
     1321 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg 
     1381 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta 
     1441 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt 
     1501 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg 
     1561 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 
     1621 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 
     1681 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 
     1741 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca 
     1801 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 
     1861 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 
     1921 tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 
     1981 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 
     2041 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 
     2101 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc 
     2161 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 
     2221 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 
     2281 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 
     2341 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 
     2401 tgaattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     2461 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     2521 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     2581 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     2641 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     2701 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     2761 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     2821 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     2881 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     2941 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     3001 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     3061 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     3121 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     3181 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     3241 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     3301 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     3361 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     3421 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     3481 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     3541 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     3601 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     3661 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     3721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     3781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     3841 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     3901 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     3961 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     4021 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     4081 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     4141 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     4201 cagtgagcga ggaagcggaa g 
//