LOCUS       pCR2.1-U6-   4220 bp    DNA   circular            10-AUG-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|376520234|
COMMENT     VNTDBDATE|382468774|
COMMENT     VNTNAME|pCR2.1-U6-2161(AS)|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|marc weinberg|
COMMENT     VNTOAUTHOREML|marco.weinberg2gmail.com|
COMMENT     VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     primer_bind     205..221
                     /vntifkey="28"
                     /label=M13\R\primer\binding\site
     promoter        complement(653..672)
                     /vntifkey="30"
                     /label=T7\promoter
     primer_bind     680..694
                     /vntifkey="28"
                     /label=M13\(-20)\F\primer\binding\site
     rep_origin      837..1273
                     /vntifkey="33"
                     /label=f1\(+)\ori
     CDS             1608..2402
                     /vntifkey="4"
                     /label=KanR
     CDS             2420..3280
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3425..4098
                     /vntifkey="21"
                     /label=pUC\ori
     promoter        complement(323..584)
                     /vntifkey="29"
                     /label=U6\promoter
     CDS             complement(294..322)
                     /vntifkey="4"
                     /label=2161(AS)
BASE COUNT     1008 a      1070 c      1105 g      1037 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaaaaaaa 
      301 actggtgtct cattgtttat acggtgtttc gtcctttcca caagatatat aaagccaaga 
      361 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta 
      421 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct 
      481 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca 
      541 aatatgaagg aatcatggga aataggccct cttcctgccc gaccaagccg aattctgcag 
      601 atatccatca cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag 
      661 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 
      721 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 
      781 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg 
      841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
      901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
      961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     1021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     1081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     1141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     1201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     1261 aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca 
     1321 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg 
     1381 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag 
     1441 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta 
     1501 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc 
     1561 gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 
     1621 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 
     1681 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 
     1741 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 
     1801 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 
     1861 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 
     1921 cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 
     1981 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 
     2041 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 
     2101 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 
     2161 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 
     2221 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 
     2281 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 
     2341 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 
     2401 gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 
     2461 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 
     2521 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 
     2581 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 
     2641 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 
     2701 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 
     2761 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 
     2821 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 
     2881 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 
     2941 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 
     3001 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 
     3061 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 
     3121 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 
     3181 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 
     3241 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 
     3301 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 
     3361 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 
     3421 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 
     3481 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 
     3541 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 
     3601 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 
     3661 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 
     3721 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 
     3781 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 
     3841 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 
     3901 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 
     3961 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 
     4021 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 
     4081 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 
     4141 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 
     4201 agtgagcgag gaagcggaag 
//