LOCUS       p4RZcGFP-a   7795 bp    DNA   circular            21-SEP-2004
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|272384737|
COMMENT     VNTDBDATE|350227103|
COMMENT     VNTNAME|p4RZcGFP-aHIVnef|
COMMENT     VNTAUTHORNAME|David J. Looney, M.D.|
COMMENT     VNTAUTHORTEL|858-552-8585x2626|
COMMENT     VNTAUTHORFAX|858-52-7416|
COMMENT     VNTAUTHOREML|dlooney@ucsd.edu|
COMMENT     VNTAUTHORAD1|Dept. of Medicine 0678|
COMMENT     VNTAUTHORAD2|University of California San Diego|
COMMENT     VNTAUTHORAD3|Stein Clinical Research Bldg Room 302|
COMMENT     VNTAUTHORAD4|La Jolla, CA 92093-0678 USA|
FEATURES             Location/Qualifiers
     LTR             complement(4070..5543)
                     /vntifkey="19"
                     /label=5'LTR
                     /note="HIV-2 5'LTR"
     LTR             complement(7643..1303)
                     /vntifkey="19"
                     /label=3'LTR
                     /note="HIV-2 3'LTR"
     misc_feature    3105..4060
                     /vntifkey="21"
                     /label=4RZ\ribozyme\cassette
                     /note="4RZ ribozyme cassette cloned from T7.1 plasmid"
     misc_feature    2783..3104
                     /vntifkey="21"
                     /label=RRE
                     /note="HIV RRE"
     misc_feature    2191..2771
                     /vntifkey="21"
                     /label=pCMV
                     /note="CMV promoter"
     misc_feature    1321..1393
                     /vntifkey="21"
                     /label=HIV-1\nef\Ribozyme
                     /note="HIV-1 nef ribozyme driven off pol II promoter"
BASE COUNT     1802 a      2026 c      1910 g      2053 t         4 others
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acgataggcc 
      421 taacaccgaa tgaccaggcg gcgactagga gagatgggaa cacacactta acttgcttct 
      481 aactggcagc tttattaaga ggtctttaag caagcaagcg tggagccgtc tgcccagtac 
      541 cggccaagta ctggtgagag tctagcaggg aacacccagg ctctacctgc tagtgctgga 
      601 gagaacctcc cagggctcga tctgccagcc tctccgcaga gcgactgaat acaatgcaag 
      661 cagcgggtac atttatacag aagtatgagg gcgttcccca ccggttcctc ccacgtccct 
      721 cccctggtta cagccccttc tggaaagtcc ctgcagtttc atcatctact tcctgttctg 
      781 accaaatata gctgttcctg tctttaacta aatggtatcc ctcttgcttt cagttttgcc 
      841 ttccactctt cttctggcag gcctgactta tacccaaatt cttctgggta tcgattaaaa 
      901 gccgtatact cataggccag cctagggtca aacctccaca ttaatgtttc cccatgaggg 
      961 tcatcagacc cgcttgtttg tgctgggtgt agtaagcagt ggtcctcttc accttgtggg 
     1021 acgtctactg gtactagctt ccatagccac ccaaagaact ttgggtacct tactcctggc 
     1081 ccatgagtat agttctgcca atctggaatt atcccttctt ccttttccat gtatatgtct 
     1141 aagattctat gtccttctct cactgtaata catcccatcc aggtcccccc tttttcattt 
     1201 agaaaatgtg atatatctac tgccaactta tatgtcattg cccttaatgg tactcttggt 
     1261 gtgacaggga cccctattag gttatcatta tctgcatcta catcatccag ttggggatcc 
     1321 ggtcacctac taacttttct tttaaaaggg gggctgatga gtccgtgagg acgaaacacc 
     1381 tatgggagga tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag 
     1441 atctgagtcc ggacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga 
     1501 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg 
     1561 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct 
     1621 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca 
     1681 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt 
     1741 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga 
     1801 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt 
     1861 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga 
     1921 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg 
     1981 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg 
     2041 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt 
     2101 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct 
     2161 tgctcaccat ggtggcgacc ggtagcgcta gcggatctga cggttcacta aaccagctct 
     2221 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt 
     2281 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc 
     2341 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta 
     2401 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt 
     2461 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt 
     2521 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt 
     2581 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg 
     2641 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc 
     2701 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt 
     2761 aattgattac tattaataac tattccaatc aggtactatg ctgttatttt cccatagtac 
     2821 agtggtgtag cagacttgtc taaatgcaca tccccatgaa tttagccgcg cctggtccct 
     2881 taggtatttc tcgatagcag tgactcttgt ctggagattt tttgttcccc agacggtcag 
     2941 tcgcaacatt tcttgttgtc tcttgaccac gtccaacagc tgttgctgtt gctgcactat 
     3001 cccagcccag taaagtccgg gatgagccga cagcgtcaag gacgccgcgc ccattgcaga 
     3061 acctgctgtt gcgagaaaac ccaagactcc tagcacaaac acattctaga tgctctctaa 
     3121 gccggcgagc gatcgcaatt caggactagt cttttaggtc aaaaagaaga agctttgtaa 
     3181 ccgttggttt ccgtagtgta gtggttatca cgttcgcctc acacgcgaac ggtccccggt 
     3241 tcgaaaccgg gcggaaacag ggccccgagg cgcatgcttg tatgcaagaa ccaaaccaga 
     3301 gaaacacacg gacttcggtc cgtggtatat tacctggtat ttttgcattt ttgcggccgc 
     3361 aattcaggac tagtctttta ggtcaaaaag aagaagcttt gtaaccgttg gtttccgtag 
     3421 tgtagtggtt atcacgttcg cctcacacgc gaacggtccc cggttcgaaa ccgggcggaa 
     3481 acacctaggc gggtagacgt cgtatgtcat tagaagtcca ccagagaaac acacgttgtg 
     3541 gtatattacc tggtattttt gcatttttgg cgccaattca ggactagtct tttaggtcaa 
     3601 aaagaagaag ctttgtaacc gttggtttcc gtagtgtagt ggttatcacg ttcgcctcac 
     3661 acgcgaacgg tccccggttc gaaaccgggc ggaaacatta attaacggcg caccggttta 
     3721 tgttagaagg tgaccagaga aacacacgga cttcggtccg tggtatatta attcaggact 
     3781 agtcttttag gtcaaaaaga agaagctttg taaccgttgg tttccgtagt gtagtggtta 
     3841 tcacgttcgc ctcacacgcg aacggtcccc ggttcgaaac cgggcggaaa cattaattaa 
     3901 cggcgcaatt gaaaaanaaa ttnganccac ngaatgagag agagatctga cacaacaaga 
     3961 aggcaaccag agaaacacac gttgtggtat attacctggt acgcgtggac gtaagtcctt 
     4021 tttgcatttt tccaaaaaat ggtctagact cgagtctaga ctagagctag agtcgagccg 
     4081 gcggtgctgc tggtctactt gtgcttggca ttttgtctgc agttcctgtt tctgccacta 
     4141 gatgtctctg tactatttgt tttgctcctt cagtatcttt cactttctct tctgcgtgca 
     4201 agcaccaaat gacgcagaca gtattaaaaa ggctttttaa attttctgat cctgttggca 
     4261 ctaatggatc taaaactgta ataatttttt ggcaaccttc ttttgactcc aacaggcttt 
     4321 ctgccaatcc gaatttgccc aattcattcg ctgcccacac aatatgcttt agtctatatt 
     4381 ttttcttccc gccgggccgt aacctaattt tttctaattc atctactttt ttccctctca 
     4441 agacggagct tctcgcgccc atctcccaca atcttctacc tgtctaaagg taggataaca 
     4501 agcctttttc tggctacagt ttttggtgta ggtacttacc ttcacccgga ggcctcttta 
     4561 ctcccgctcc acacgccgcc tttggtacct cggcccgcgc ctttctagga gcactccgtc 
     4621 gtggtttgtt cctgccgccc ttactgcctt cactcagccg tgttccagtc tccttcaagt 
     4681 cctgttcggg cgccaatctg ctaggtattt tcccgcttcg gttccccaaa gcaagaaggg 
     4741 tcctaacaga ccagggtctt gtcacttagg tgaacaccga atgaccaggc ggcgactagg 
     4801 agagatggga acacacactt aacttgcttc taactggcag ctttattaag aggtctttaa 
     4861 gcaagcaagc gtggagccgt ctgcccagta ccggccaagt actggtgaga gtctagcagg 
     4921 gaacacccag gctctacctg ctagtgctgg agagaacctc ccagggctcg atctgccagc 
     4981 ctctccgcag agcgactgaa tacaatgcaa gcagcgggta catttataca gaagtatgag 
     5041 ggcgttcccc accggttcct cccacgtccc tcccctggtt acagcccctt ctggaaagtc 
     5101 cctgcagttt catcatctac ttcctgttct gaccaaatat agctgttcct gtctttaact 
     5161 aaatggtatc cctcttgctt tcagttttgc cttccactct tcttctggca ggcctgactt 
     5221 atacccaaat tcttctgggt atcgattaaa agccgtatac tcataggcca gcctagggtc 
     5281 aaacctccac attaatgttt ccccatgagg gtcatcagac ccgcttgttt gtgctgggtg 
     5341 tagtaagcag tggtcctctt caccttgtgg gacgtctact ggtactagct tccatagcca 
     5401 cccaaagaac tttgggtacc ttactcctgg cccatgagta tagttctgcc aatctggaat 
     5461 tatcccttct tccttttcca tgtatatgtc taagattcta tgtccttctc tcactgtaat 
     5521 acatcccatc caacgcgttc gacctgcagg catgcaagct tggcgtaatc atggtcatag 
     5581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
     5641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     5701 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     5761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     5821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     5881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     5941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     6001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     6061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     6121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca aagctcacgc 
     6181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     6241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     6301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     6361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
     6421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     6481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     6541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     6601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     6661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     6721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     6781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     6841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     6901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     6961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     7021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     7081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     7141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     7201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     7261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     7321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     7381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     7441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     7501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     7561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     7621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     7681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 
     7741 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc 
//